Genome scan of \u3ci\u3eDiabrotica virgifera virgifera\u3c/i\u3e for genetic variation associated with crop rotation tolerance

Crop rotation has been a valuable technique for control of Diabrotica virgifera virgifera for almost a century. However, during the last two decades, crop rotation has ceased to be effective in an expanding area of the US corn belt. This failure appears to be due to a change in the insect’s oviposition behaviour, which, in all probability, has an underlying genetic basis. A preliminary genome scan using 253 amplified fragment-length polymorphism (AFLP) markers sought to identify genetic variation associated with the circumvention of crop rotation. Samples of D. v. virgifera from east-central Illinois, where crop rotation is ineffective, were compared with samples from Iowa at locations that the behavioural variant has yet to reach. A single AFLP marker showed signs of having been influenced by selection for the circumvention of crop rotation. However, this marker was not diagnostic. The lack of markers strongly associated with the trait may be due to an insufficient density of marker coverage throughout the genome. A weak but significant general heterogeneity was observed between the Illinois and Iowa samples at microsatellite loci and AFLP markers. This has not been detected in previous population genetic studies of D. v. virgifera and may indicate a reduction in gene flow between variant and wild-type beetles

Peaks in the Hartle-Hawking Wave Function from Sums over Topologies

Recent developments in ``Einstein Dehn filling'' allow the construction of infinitely many Einstein manifolds that have different topologies but are geometrically close to each other. Using these results, we show that for many spatial topologies, the Hartle-Hawking wave function for a spacetime with a negative cosmological constant develops sharp peaks at certain calculable geometries. The peaks we find are all centered on spatial metrics of constant negative curvature, suggesting a new mechanism for obtaining local homogeneity in quantum cosmology.Comment: 16 pages,LaTeX, no figures; v2: some changes coming from revision of a math reference: wave function peaks sharp but not infinite; v3: added paragraph in intro on interpretation of wave functio

Early and Sustained Supramarginal Gyrus Contributions to Phonological Processing

Reading is a difficult task that, at a minimum, requires recognizing a visual stimulus and linking it with its corresponding sound and meaning. Neurologically, this involves an anatomically distributed set of brain regions cooperating to solve the problem. It has been hypothesized that the supramarginal gyrus (SMG) contributes preferentially to phonological aspects of word processing and thus plays an important role in visual word recognition. Here, we used chronometric transcranial magnetic stimulation (TMS) to investigate the functional specificity and timing of SMG involvement in reading visually presented words. Participants performed tasks designed to focus on either the phonological, semantic, or visual aspects of written words while double pulses of TMS (delivered 40 ms apart) were used to temporarily interfere with neural information processing in the left SMG at five different time windows. Stimulation at 80/120, 120/160, and 160/200 ms post-stimulus onset significantly slowed subjects’ reaction times in the phonological task. This inhibitory effect was specific to the phonological condition, with no effect of TMS in the semantic or visual tasks, consistent with claims that SMG contributes preferentially to phonological aspects of word processing. The fact that the effect began within 80–120 ms of the onset of the stimulus and continued for approximately 100 ms, indicates that phonological processing initiates early and is sustained over time. These findings are consistent with accounts of visual word recognition that posit parallel activation of orthographic, phonological, and semantic information that interact over time to settle into a distributed, but stable, representation of a word

Utility of EST-Derived SSRs as Population Genetics Markers in a Beetle

Microsatellite, or simple sequence repeat (SSR), loci can be identified by mining expressed sequence tag (EST) databases, and where these are available, marker development time and expense can be decreased considerably over conventional strategies of probing the entire genome. However, it is unclear whether they provide information on population structure similar to that generated by anonymous genomic SSRs. We performed comparative population genetic analyses between EST-derived SSRs (EST-SSRs) and anonymous SSRs developed from genomic DNA for the same set of populations of the insect Diabrotica virgifera, a beetle in the family Chrysomelidae. Compared with noncoding, nontranscribed regions, EST-SSRs were generally less polymorphic but had reduced occurrence of null alleles and greater cross-species amplification. Neutrality tests suggested the loci were not under positive selection. Across all populations and all loci, the genomic and EST-SSRs performed similarly in estimating genetic diversity, FIS, FST, population assignment and exclusion tests, and detection of distinct populations. These findings, therefore, indicate that the EST-SSRs examined can be used with confidence in future genetic studies of Diabrotica populations and suggest that EST libraries can be added as a valuable source of markers for population genetics studies in insects and other animals

Genome scan of Diabrotica virgifera virgifera for genetic variation associated with crop rotation tolerance

Crop rotation has been a valuable technique for control of Diabrotica virgifera virgifera for almost a century. However, during the last two decades, crop rotation has ceased to be effective in an expanding area of the US corn belt. This failure appears to be due to a change in the insect's oviposition behaviour, which, in all probability, has an underlying genetic basis. A preliminary genome scan using 253 amplified fragment-length polymorphism (AFLP) markers sought to identify genetic variation associated with the circumvention of crop rotation. Samples of D. v. virgifera from east-central Illinois, where crop rotation is ineffective, were compared with samples from Iowa at locations that the behavioural variant has yet to reach. A single AFLP marker showed signs of having been influenced by selection for the circumvention of crop rotation. However, this marker was not diagnostic. The lack of markers strongly associated with the trait may be due to an insufficient density of marker coverage throughout the genome. A weak but significant general heterogeneity was observed between the Illinois and Iowa samples at microsatellite loci and AFLP markers. This has not been detected in previous population genetic studies of D. v. virgifera and may indicate a reduction in gene flow between variant and wild-type beetles

Tamoxifen, aminoglutethimide and danazol: effect of therapy on hormones in post-menopausal patients with breast cancer.

Gonadotrophins, oestradiol, androstenedione, testosterone and dehydroepiandrosterone sulphate (DHAS) were measured sequentially in 72 patients with advanced breast cancer receiving endocrine therapy of various types. Tamoxifen significantly reduced gonadotrophins but did not effect other hormones. Danazol also reduced gonadotrophins. Aminoglutethimide (AGT) reduced oestradiol and DHAS but had not effect on gonadotrophins. The effects of administering tamoxifen, AGT and danazol together (TAD) together were therefore examined. This combination reduced gonadotrophins, oestradiol and DHAS, but no further than tamoxifen and AGT alone. The degree and duration of hormone suppression were similar in both responders and non-responders to tamoxifen, AGT or TAD, though patients responding to AGT showed more complete suppression at the end of the course of treatment, perhaps because they were treated longer. On relapse, adequate gonadotrophin and steroid suppression was demonstrated in patients receiving tamoxifen and AGT respectively. We conclude that (a) response to endocrine therapy is unlikely to be related to the degree of endocrine suppression produced by the therapy; (b) combination endocrine therapy does not further reduce serum-hormone concentrations and (c) relapse is unlikely to be due to escape from the hormone-inhibitory effects of endocrine agents

Circular Polarization Induced by Scintillation in a Magnetized Medium

A new theory is presented for the development of circular polarization as radio waves propagate through the turbulent, birefringent interstellar medium. The fourth order moments of the wavefield are calculated and it is shown that unpolarized incident radiation develops a nonzero variance in circular polarization. A magnetized turbulent medium causes the Stokes parameters to scintillate in a non-identical manner. A specific model for this effect is developed for the case of density fluctuations in a uniform magnetic field.Comment: 16 pages, 1 figure, Phys. Rev. E, accepte

Drell-Yan forward-backward and spin asymmetries for arbitrary vector boson production at next-to-leading order

Longitudinally polarized, unpolarized and forward-backward mass differential cross sections for Drell-Yan lepton-pair production by arbitrary vector bosons are calculated in next-to-leading order (NLO) QCD. Analytical results are presented in a form valid for all consistent $n$-dimensional regularization schemes, with the mass factorization scheme kept general. NLO predictions for all Drell-Yan type processes ($W^\pm$, $Z$ and $\gamma^*$) at BNL's relativistic heavy ion collider (RHIC) are made using polarized parton distributions which fit the recent deep-inelastic scattering data. These are examined as tools in the determination of the polarized parton distributions and the unpolarized $\bar{u}/\bar{d}$ ratio. NLO predictions for the forward-backward lepton asymmetry at Fermilab are made and the precision determination of $\sin^2 \theta_W$ from future runs is studied. In all the above, the QCD corrections are found to be significant. An introductory discussion is given of various theoretical issues, such as allowable factorization and regularization schemes, and scale dependences.Comment: 34 pages, figures included, revtex. Some discussions and references added/modified. In more compact form. To appear in Phys. Rev.

Dominant Topologies in Euclidean Quantum Gravity

The dominant topologies in the Euclidean path integral for quantum gravity differ sharply according on the sign of the cosmological constant. For $\Lambda>0$, saddle points can occur only for topologies with vanishing first Betti number and finite fundamental group. For $\Lambda<0$, on the other hand, the path integral is dominated by topologies with extremely complicated fundamental groups; while the contribution of each individual manifold is strongly suppressed, the ``density of topologies'' grows fast enough to overwhelm this suppression. The value $\Lambda=0$ is thus a sort of boundary between phases in the sum over topologies. I discuss some implications for the cosmological constant problem and the Hartle-Hawking wave function.Comment: 14 pages, LaTeX. Minor additions (computability, relation to ``minimal volume'' in topology); error in eqn (3.5) corrected; references added. To appear in Class. Quant. Gra