Point Prevalence of Gastrointestinal Helminthiaisis in Buffaloes of Jammu, India

The present study was carried out to determine the prevalence of gastrointestinal helminthiasis in buffaloes of Jammu area of Jammu And Kashmir State for the period of one year. For this purpose, 173 faecal samples were collected from different areas (Bisnah, R S Pura, Sidrah, Jammu city and Akhnoor) of subtropical Jammu. Parasitological procedures used for the identification of helminthes were direct and indirect methods. The overall prevalence of helminthiasis was 38.72%. Helminthic infection was recorded throughout the year with seasonal variations

Studies on the histochemistry of Paramphistomes of sheep and cattle.

Sheep and cattle constitute one of the premier economic group, providing animal protein which is indispensable in regular staple diet of common man in our country, especially in native state of Jammu and Kashmir. The present study, which deals with the histochemistry of Paramphistomes of sheep and cattle, was undertaken from January 2004 to January 2007. Since there has been no detailed study on the histochemistry of helminths particularly Paramphistomes in Kashmir valley, the present study is a step towards bridging the gap. Sheep and cattle like other animals develop diseases due to variety of parasitic infections, resulting in huge economic losses. Fascioliasis and Paramphistomiasis rank high in significance of helminth diseases of sheep and cattle because they cause heavy mortality and morbidity in young cattle and sheep. Various species of Paramphistomes such as Paramphistomum cervi, Paramphistomum microbothrium, Paramphistomum skrajabini, Cotylophoron cotylophorum, Gastrothylax crumenifer and Gigantocotyle explanatum have been incriminated as aetiological agents of Paramphistomiasis. In order to develop various control measures to these helminths we should have a clear understanding of host parasite relationship and a thorough understanding of the host parasite relationship cannot be realized until a careful study is made on the biochemical nature of the parasite and its host. Histochemical study helps us to investigate qualitatively the biochemical pattern of different tissues in cellular architecture. The present work demonstrated the presence of general proteins, glycogen, lipids, cholinesterase, acid phosphatase, alkaline phosphatase and calcium in Cotylophoron cotylophorum collected from cattle in comparison with Paramphistomum cervi collected from sheep.Digital copy of Thesis.University of Kashmir

Identification of novel interacts partners of ADAR1 enzyme mediating the oncogenic process in aggressive breast cancer

Abstract Triple-negative breast cancer (TNBC) subtype is characterized by aggressive clinical behavior and poor prognosis patient outcomes. Here, we show that ADAR1 is more abundantly expressed in infiltrating breast cancer (BC) tumors than in benign tumors. Further, ADAR1 protein expression is higher in aggressive BC cells (MDA-MB-231). Moreover, we identify a novel interacting partners proteins list with ADAR1 in MDA-MB-231, using immunoprecipitation assay and mass spectrometry. Using iLoop, a protein–protein interaction prediction server based on structural features, five proteins with high iloop scores were discovered: Histone H2A.V, Kynureninase (KYNU), 40S ribosomal protein SA, Complement C4-A, and Nebulin (ranged between 0.6 and 0.8). In silico analysis showed that invasive ductal carcinomas had the highest level of KYNU gene expression than the other classifications (p < 0.0001). Moreover, KYNU mRNA expression was shown to be considerably higher in TNBC patients (p < 0.0001) and associated with poor patient outcomes with a high-risk value. Importantly, we found an interaction between ADAR1 and KYNU in the more aggressive BC cells. Altogether, these results propose a new ADAR-KYNU interaction as potential therapeutic targeted therapy in aggressive BC