Purification and properties of phage P22-induced lysozyme

Phage P22 induces a lysozyme in Salmonella typhimurium cells toward the later stage of its multiplication. P22 lysozyme has been purified about 1000-fold starting from the lysate of C1 (clear plaque-forming mutant of phage P22)-infected cells. The enzyme has an optimum pH between 7 and 8 and its activity is dependent on the ionic strength of the assay medium. Salts like MgCl2, NaCl, and KCl are inhibitory to the lysozyme. Gram-negative cells act as better substrates for the lysozyme than do gram-positive cells. The enzyme has a molecular weight of about 2 × 104 and rapidly looses its activity at temperatures higher than 40°. The properties of P22 lysozyme have been compared with those of λ and T4 lysozymes. All three lysozymes have more or less the same molecular weight and have similar properties although P22 and T4 lysozymes seem to be closer than P22 and λ lysozymes

Closest Keyword Search in Dynamic Multidimensional Data Sets

Adding text to databases opens up many different innovations and functionalities that can be made feasible for keyword-based quests. The application in question focuses on search results that are keyword-marked and that are located in a geographical area. For these datasets, our main goal is to locate groups of points that satisfy search queries. Our team's recommendation is a process we call Projection and Multi Scale Hashing that combines random projection and hashing to provide great scalability and efficiency. This example illustrates how to present algorithms in both an exact and approximate manner. Analyses that take into account experimental and analytical studies show that, with regard to overall efficiency, multi-dimensional hashing offers up to 65 times better results. A point in a dynamic connection multi-dimensional feature space is a typical way to classify an object, and we often describe various objects as a point in a multi-dimensional feature space. In other words, for example, images are described using feature vectors that are comprised of colour components, and a textual description of the image is typically correlated with it (such as tags or keywords)

Syntheses in the cadinane series: synthesis of (±)-cadinene dihydrochloride

This article does not have an abstract

ENHANCING THE IMPREGNABILITY OF LINUX SERVERS

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