A Bacteriological Study of Intertrigo

The study was taken up to identify the various bacterial agents causing intertrigo of feet and to determine the antimicrobial sensitivity pattern of the isolated bacterial agents. A total of 75 pus samples were collected from infected toe, web space infection by using sterile swabs.Samples were inoculated into Blood agar and MacConkey agar for the bacterial isolation and antibiotic susceptibility testing was performed as per CLSI Guidelines. Out of 75 samples examined 38 were from male patients and 37 were collected from female patients. 101 different types of bacteria were grown in the present study. Among them38 Gram-PositiveCocci (GPC) and 63 Gram-Negative Bacilli (GNB). Of 38 Gram positive cocci isolated, Staphylococcus aureuswere19 (18.81%), followed by Coagulase negative staphylococcus (CONS) 12 (11.88%) and Streptococcus spp7 (6.93%). Of 63 Gram negative bacilli isolated Pseudomonas were19 (18.81%), followed by Proteus 16 (15.84%) and Klebsiella13 (12.87%). 25.8% Staphylococcus sppare Methecillin Resistant (MR) strains. Among the Gram negative bacteria 9.25% of the strains were showing resistance to Colistin or Tigecycline. It is known fact that different microbes and factors play very important role in lesions of toe web space. In the present study 25.8% of Staphylococcus species and 9.25% of Gram negative bacteria were multi drug resistant.Therefore, clinical and microbiologic studies are needed to help in the selection of appropriate treatment, prevention of important complications and emergence of drug resistance pathogens

Bacteriological Profile of Endotracheal Aspirates and their Antibiotic Susceptibility Pattern

Early onset Ventilator associated pneumonia (VAP) is usually less severe, associated with a better prognosis, and is more likely caused by antibiotic- sensitive bacteria. Late-onset VAP, is usually caused by multidrug resistant (MDR) pathogens and is associated with increased morbidity and mortality. Therefore, the local microbial flora causing VAP needs to be studied and appropriate therapy based on the early endotracheal (ET) aspirate culture report can help managing this group of patients. The present study was carried out in the department of Microbiology, JSS Hospital, Mysore from January 2017 to December 2017. Bacterial pathogens and antibiotic resistance pattern of isolates from ET aspirates of patients on mechanical ventilation in Intensive care units were source of data. A total number of 1432 samples were received in the lab for ET aspirate culture. Among these 1432 samples, 1055 showed growth ranging from 10 2 to 106 colony forming units/ml (CFU/ml), 124 had no growth at all and 253 had no significant growth (<102 CFU/ml) Out of 1055 isolates,1023 were Gram negative bacteria and 32 were Gram positive cocci . Acinetobacter, Pseudomonas and Klebsiella were the major pathogens in our study. Majority of them being MDR, maximum sensitivity was observed for Tigecycline and colistin in these isolates. Knowledge of causative microbial flora of VAP along with information on the susceptibility patterns will help in selection of the appropriate antibiotic for therapeutic use for better outcome