Additional file 3: of Read-Split-Run: an improved bioinformatics pipeline for identification of genome-wide non-canonical spliced regions using RNA-Seq data

Statistics reported by RSR for 100 test cases for the Tg sample. This file contains all supplementary results for 100 test cases for the Tg sample. (XLSX 92 kb

Additional file 5: of Read-Split-Run: an improved bioinformatics pipeline for identification of genome-wide non-canonical spliced regions using RNA-Seq data

Total number of spliced regions identified by RSR in the human ENCODE RNA-Seq dataset. This file includes all supplementary results for number of supporting reads, splice length, range of supporting reads (spliced regions) identified by RSR in the human ENCODE RNA-Seq dataset. (XLSX 1411 kb

Epimedium sempervirens Nakai

原著和名: トキハイカリサウ科名: メギ科 = Berberidaceae採集地: 島根県 隠岐 島後 (隠岐 島後)採集日: 1976/5/9採集者: 萩庭丈壽整理番号: JH002351国立科学博物館整理番号: TNS-VS-95235

Science and society: in the sixteenth and seventeenth centuries

eIF2α phosphorylation in hepatocytes is dispensable for survival of adult mice. (a) Diagram depicting the four genotypes of mice used in these experiments. S/A and A/A represent heterozygous and homozygous eIF2α Ser51Ala (*) mutation(s) in exon 2 of one eIF2α allele and both eIF2α alleles, respectively. fTg/0 represents the floxed wild type (WT) eIF2α transgene driven by the CMV enhancer and chicken β-actin promoter (Enh-Pro). The loxP sequences (black arrowheads) allow excision of the WT eIF2α floxed transgene (fTg) and expression of EGFP, an indicator of recombination. CRE Hep /0 represents the Cre recombinase transgene driven by the promoter (Alfp) of Alb1 (encoding albumin) and the enhancer of Afp (encoding alpha-fetoprotein). (b) Efficiency of deletion of the fTg in liver tissues. Results from quantitative RT-PCR analyses of transgenic and total eIF2α mRNAs are shown. Data are means ± SEM (n = 4 ~ 5 mice per group); ### p < 0.001; Cont. vs A/A Hep . (c) Western blot analysis of eIF2α protein expression driven by the fTg in liver tissues. To quantity expression of eIF2α, blots were incubated with anti-eIF2α antibody followed by IRDye-800 goat anti-rabbit IgG (LI-COR). Membranes were scanned on an Odyssey scanner (LI-COR) (lower two panels in left panels) and quantified with the Odyssey Software package. (d) Western blot analysis of liver lysates in Cont. and A/A Hep mice at the indicated times after Tm injection. Cont. mice and A/A Liv mice were injected with vehicle or tunicamycin (Tm, 1 mg/kg). (e) Body weight measurements of fTg -deleted A/A Liv mice. At the weeks, body weight was measured in both male and female mice. Data are means ± SEM (n = 6-14 mice per group). (PDF 1987 kb

Additional file 2: Table S2. of eIF2Îą phosphorylation is required to prevent hepatocyte death and liver fibrosis in mice challenged with a high fructose diet

PCR primers. (XLS 40 kb