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Not AvailableOBJECTIVES:
The occurrence of Listeria monocytogenes was studied by using cultural and serological methods in cattle housed in a particular gaushala (cattle shelter) and organized dairy farm.
MATERIALS AND METHODS:
A total of 1201 samples from cattle comprising blood (nβ=β207), milk (nβ=β203), vaginal swabs (nβ=β210), and serum (nβ=β207) from an organized farm (nβ=β210) and blood (nβ=β100), milk (nβ=β74), vaginal swabs (nβ=β100), and serum (nβ=β100) from a gaushala (nβ=β100) were collected and analyzed for L. monocytogenes. All samples excluding serum were analyzed for isolation and identification of L. monocytogenes, while the serum samples were screened for seropositivity. The isolates were further subjected to assess their virulence potential (in vitro and in vivo), biofilm formation ability, and antibiotic susceptibility patterns.
RESULTS:
Four L. monocytogenes strains were isolated from the cattle; three (0.48%) from the organized farm and one (0.36%) from the gaushala. On serological screening of cattle from the organized dairy farm, 16.42% were found to be positive for antibodies against listeriolysin O, while cattle from the gaushala revealed 36% seropositivity. Furthermore, on characterization of the isolates for their pathogenic potential and biofilm-forming ability, all were found to be pathogenic by both in vitro and in vivo assays and were weak to moderate biofilm formers. The minimum inhibition concentration (MIC) of recovered isolates revealed resistance for ampicillin by two L. monocytogenes isolates (MIC >256βΞΌg/mL), whereas three L. monocytogenes isolates were intermediately resistant (MIC >4βΞΌg/mL) and one resistant against amoxicillin (MIC >8βΞΌg/mL). However, all four isolates were susceptible to gentamicin, cotrimoxazole, and erythromycin.Not Availabl