Acute toxicity studies of polyherbal formulation (Methiorep Premix)

An experimental study with the objective to evaluate the acute toxicity of polyherbal formulation Methiorep Premix (supplied by Ayurvet Ltd., Baddi, India), was done as per OECD guidelines 423. Methiorep is a scientifically developed combination of herbs that contains herbal ingredients rich in Methionine in free form as well as conjugated form. The study was done on 3 males and 3 female Wistar Albino rats which were administered an initial dose of 500 mg/kg body weight followed by 2000 mg/kg & 5000 mg/kg body weight. The animals were observed for signs of convulsions, tremors, circling, depression, excitement and mortality. Body weight was recorded at 0,7th and 14th day and plasma total protein, albumin; AST and ALT were measured to evaluate the toxicity of the preparation. No abnormal sign of symptoms were observed in any of the animal fed with Methiorep Premix at the dose rate of 500 mg/kg body weight, 2000 mg/kg body weight & 5000mg/kgbody weight. No mortality was observed in any of the animals indicating its safety. [Vet. World 2009; 2(2.000): 58-59