2 research outputs found
Not Available
Get PDFNot AvailableDeoxyribonucleic acid barcoding, using mitochondrial
cytochrome c oxidase-1 sequence, is an accurate
and rapid identification method for insect-taxa and independent
of life-stages, sex and polymorphism. The present
study aimed at providing deoxyribonucleic acid barcode
for the correct identification of Stromatium barbatum
(Fabricius), a new pest of grapevines in India. Morphometric
analysis and pictorial illustrations of important
morphological features and life-stages were also documented
for future reference. Average length and breadth (in
mm) were 1.012 ± 0.10 and 0.49 ± 0.07 for egg,
1.52 ± 0.15 and 0.50 ± 0.04 for neonate-grub,
35.12 ± 0.47 and 7.08 ± 0.12 for fully matured grub,
26.0 ± 0.25 and 8.64 ± 0.12 for pupa, 21.20 ± 2.51 and
6.02 ± 0.62 for adult-male, and 22.47 ± 2.26 and
6.67 ± 0.75 for adult-female, respectively. Antennae was
filiform which was longer in males (30.63 ± 5.89 mm) than females (22.55 ± 4.84 mm). The barcode showed
86% match with closely related S. longicorne and in
Neighbor Joining analysis formed monophyletic clade with
S. longicorne suggesting correct genus-level identification.
Specimens used for sequencing were morphologically
identified by an expert taxonomist and compared with type
specimens before barcoding and sequences were submitted
to National Center for Biotechnology Information. The
barcode was put to test for confirmation of size-polymorphism.
Cytochrome c oxidase-1 regions were sequenced
for morphologically identified S. barbatum large male
(2.6 cm), large female (2.9 cm), small male (1.6 cm) and
small female (1.8 cm). These sequences showed B2.5%
divergence among each other and B2.5 divergence with the
DNA barcode confirming that the four specimens of different
sizes belonged to same species and variation was due
to size-polymorphism.Not Availabl
Not Available
No full textNot AvailableDeoxyribonucleic acid barcoding, using mitochondrial
cytochrome c oxidase-1 sequence, is an accurate
and rapid identification method for insect-taxa and independent
of life-stages, sex and polymorphism. The present
study aimed at providing deoxyribonucleic acid barcode
for the correct identification of Stromatium barbatum
(Fabricius), a new pest of grapevines in India. Morphometric
analysis and pictorial illustrations of important
morphological features and life-stages were also documented
for future reference. Average length and breadth (in
mm) were 1.012 ± 0.10 and 0.49 ± 0.07 for egg,
1.52 ± 0.15 and 0.50 ± 0.04 for neonate-grub,
35.12 ± 0.47 and 7.08 ± 0.12 for fully matured grub,
26.0 ± 0.25 and 8.64 ± 0.12 for pupa, 21.20 ± 2.51 and
6.02 ± 0.62 for adult-male, and 22.47 ± 2.26 and
6.67 ± 0.75 for adult-female, respectively. Antennae was
filiform which was longer in males (30.63 ± 5.89 mm) than females (22.55 ± 4.84 mm). The barcode showed
86% match with closely related S. longicorne and in
Neighbor Joining analysis formed monophyletic clade with
S. longicorne suggesting correct genus-level identification.
Specimens used for sequencing were morphologically
identified by an expert taxonomist and compared with type
specimens before barcoding and sequences were submitted
to National Center for Biotechnology Information. The
barcode was put to test for confirmation of size-polymorphism.
Cytochrome c oxidase-1 regions were sequenced
for morphologically identified S. barbatum large male
(2.6 cm), large female (2.9 cm), small male (1.6 cm) and
small female (1.8 cm). These sequences showed B2.5%
divergence among each other and B2.5 divergence with the
DNA barcode confirming that the four specimens of different
sizes belonged to same species and variation was due
to size-polymorphism.Not Availabl
