Multi-scale modeling of angiotensin II induced neuronal regulatory mechanisms in the brain

Poster Presentation

Incorporating Fault-Tolerant Features into Message-Passing Middleware

The popularity of MPI-based middleware and applications has led to their wide deployment. Such systems, however, are not inherently reliable and cannot tolerate external faults. This thesis presents a novel model-based approach for exploiting application features and other characteristics to categorize and create AEMs (Application Execution Model). This work realizes MPI/FT(tm), a middleware derived by selective incorporation of fault-tolerant features into MPI/Pro(tm) for two relevant AEMs. This thesis proves the following hypothesis: it is possible to successfully complete select MPI applications even in the presence of external faults, and such fault-tolerance can be achieved with acceptable performance overhead. This work defines parameters to measure the impact of this middleware on performance through faultree and fault-injected overheads. The hypothesis is validated through experimentation and measurement of sample MPI applications for two AEMs

IN VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY OF MICRO PROPAGATED CALLUS OF CURUULIGO ORCHIODES (BLACK MUSILLI) AGAR WELL DIFFUSION AND MINIMUM INHIBITORY CONCENTRATION (MIC)

Objective: In vitro investigated the potential of methanol extracts of micro-propagated C. orchiodes in the antimicrobial property against the three gram-negative bacteria, two gram-positive and one fungal filament.Methods: The micro propagated callus methanol extract was examined against Escherichia coli, Proteus vulgaris, Salmonella typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus and Candida albicans. The zone of inhibitions are determined at 10 mg/ml concentration of methanol extracts of callus on agar well plate and MIC against tested microorganism.Results: The highest antibacterial activity recorded in Staphylococcus aureus Bacillus cereus and followed by Candida albicans. Antibacterial activity of leaf extracts of A. reticulata was also significant against the tested microorganisms Escherichia coli, Salmonella typhi, Proteus vulgaris, Pseudomonas aeruginosa compared to ciprofloxacin.Conclusion: Based on the above observations, these extracts were further evaluated for their effect on microorganisms causing infections like typhoid fever, urinary tract infections, septicemia, toxic shock syndrome, skin infection, nosocomial infection, arthritis and diarrhoea. The results also suggest that these plants serve a therapeutic purpose in the treatment bacterial infections

Encryption Based Access Control Model In Cloud: A Survey

Cloud computing is known as “Utility”. Cloud Computing enabling users to remotely store their data in a server and provide services on-demand. Since this new computing technology requires user to entrust their valuable data to cloud providers, there have been increasing security and privacy concerns on outsourced data. We can increase security on access of the data in the cloud. Morever we can provide encryption on the data so third party can not use thedata. In this paper we will be reviewing various encryption based access control model for enhancing cloud security along with their limitations. We will be concluding with a proposed access control model to enhance cloud security

Chronic ethanol feeding alters miRNA expression dynamics during liver regeneration.

BACKGROUND: Adaptation to chronic ethanol (EtOH) treatment of rats results in a changed functional state of the liver and greatly inhibits its regenerative ability, which may contribute to the progression of alcoholic liver disease. METHODS: In this study, we investigated the effect of chronic EtOH intake on hepatic microRNA (miRNA) expression in male Sprague-Dawley rats during the initial 24 hours of liver regeneration following 70% partial hepatectomy (PHx) using miRNA microarrays. miRNA expression during adaptation to EtOH was investigated using RT-qPCR. Nuclear factor kappa B (NFκB) binding at target miRNA promoters was investigated with chromatin immunoprecipitation. RESULTS: Unsupervised clustering of miRNA expression profiles suggested that miRNA expression was more affected by chronic EtOH feeding than by the acute challenge of liver regeneration after PHx. Several miRNAs that were significantly altered by chronic EtOH feeding, including miR-34a, miR-103, miR-107, and miR-122 have been reported to play a role in regulating hepatic metabolism and the onset of these miRNA changes occurred gradually during the time course of EtOH feeding. Chronic EtOH feeding also altered the dynamic miRNA profile during liver regeneration. Promoter analysis predicted a role for NFκB in the immediate-early miRNA response to PHx. NFκB binding at target miRNA promoters in the chronic EtOH-fed group was significantly altered and these changes directly correlated with the observed expression dynamics of the target miRNA. CONCLUSIONS: Chronic EtOH consumption alters the hepatic miRNA expression profile such that the response of the metabolism-associated miRNAs occurs during long-term adaptation to EtOH rather than as an acute transient response to EtOH metabolism. Additionally, the dynamic miRNA program during liver regeneration in response to PHx is altered in the chronically EtOH-fed liver and these differences reflect, in part, differences in miRNA expression between the EtOH-adapted and control livers at the baseline state prior to PHx

HASBE access control model with Secure Key Distribution and Efficient Domain Hierarchy for cloud computing

Cloud computing refers to the application and service that run on a distributed system using virtualized resources and access by common internet protocol and networking standard. Cloud computing virtualizes system by pooling and sharing resources. System and resources can be monitored from central infrastructure as needed. It requires high security because now day’s companies are placing more essential and huge amount of data on cloud. Hence traditional access control models are not sufficient for cloud computing applications. So encryption based on Attribute (“ABE”-“Attribute based encryption”) has been offered for access control of subcontracted data in cloud computing with complex access control policies. Traditional HASBE provides Flexibility, scalability and fine-grained access control but does not support hierarchical domain structure. In this paper, we had enhanced “Hierarchical attribute-set-based encryption” (“HASBE”) access control with a hierarchical assembly of users, with flexible domain Hierarchy structure and Secure key distribution with predefined polic

Temporal and functional profile of the transcriptional regulatory network in the early regenerative response to partial hepatectomy in the rat

<p>Abstract</p> <p>Background</p> <p>The goal of these studies was to characterize the transcriptional network regulating changes in gene expression in the remnant liver of the rat after 70% partial hepatectomy (PHx) during the early phase response including the transition of hepatocytes from the quiescent (G₀) state and the onset of the G₁phase of the cell cycle.</p> <p>Results</p> <p>The transcriptome of remnant livers was monitored at 1, 2, 4, and 6 hours after PHx using cDNA microarrays. Differentially regulated genes were grouped into six clusters according their temporal expression profiles. Promoter regions of genes in these clusters were examined for shared transcription factor binding sites (TFBS) by comparing enrichment of each TFBS relative to a reference set using the Promoter Analysis and Interaction Network Toolset (PAINT).</p> <p>Analysis of the gene expression time series data using ANOVA resulted in a total of 309 genes significantly up- or down-regulated at <it>any </it>of the four time points at a 20% FDR threshold. Sham-operated animals showed no significant differential expression. A subset of the differentially expressed genes was validated using quantitative RT-PCR. Distinct sets of TFBS could be identified that were significantly enriched in each one of the different temporal gene expression clusters. These included binding sites for transcription factors that had previously been recognized as contributing to the onset of regeneration, including NF-κB, C/EBP, HNF-1, CREB, as well as factors, such as ATF, AP-2, LEF-1, GATA and PAX-6, that had not yet been recognized to be involved in this process. A subset of these candidate TFBS was validated by measuring activation of corresponding transcription factors (HNF-1, NK-κB, CREB, C/EBP-α and C/EBP-β, GATA-1, AP-2, PAX-6) in nuclear extracts from the remnant livers.</p> <p>Conclusion</p> <p>This analysis revealed multiple candidate transcription factors activated in the remnant livers, some known to be involved in the early phase of liver regeneration, and several not previously identified. The study describes the predominant temporal and functional elements to which these factors contribute and demonstrates the potential of this novel approach to define the functional correlates of the transcriptional regulatory network driving the early response to partial hepatectomy.</p