Association of SUMOlation Pathway Genes With Stroke in a Genome-wide Association Study in India

OBJECTIVE: To undertake a genome-wide association study (GWAS) to identify genetic variants for stroke in an Indian population. METHODS: In a hospital-based case-control study, 8 teaching hospitals in India recruited 4,088 participants, including 1,609 stroke cases. Imputed genetic variants were tested for association with stroke subtypes using both single-marker and gene-based tests. Association with vascular risk factors was performed with logistic regression. Various databases were searched for replication, functional annotation, and association with related traits. Status of candidate genes previously reported in the Indian population was also checked. RESULTS: Associations of vascular risk factors with stroke were similar to previous reports and show modifiable risk factors such as hypertension, smoking, and alcohol consumption as having the highest effect. Single-marker–based association revealed 2 loci for cardioembolic stroke (1p21 and 16q24), 2 for small vessel disease stroke (3p26 and 16p13), and 4 for hemorrhagic stroke (3q24, 5q33, 6q13, and 19q13) at p < 5 × 10(−8). The index single nucleotide polymorphism of 1p21 is an expression quantitative trait locus (p(lowest) = 1.74 × 10(−58)) for RWDD3 involved in SUMOylation and is associated with platelet distribution width (1.15 × 10(−9)) and 18-carbon fatty acid metabolism (p = 7.36 × 10(−12)). In gene-based analysis, we identified 3 genes (SLC17A2, FAM73A, and OR52L1) at p < 2.7 × 10(−6). Eleven of 32 candidate gene loci studied in an Indian population replicated (p < 0.05), and 21 of 32 loci identified through previous GWAS replicated according to directionality of effect. CONCLUSIONS: This GWAS of stroke in an Indian population identified novel loci and replicated previously known loci. Genetic variants in the SUMOylation pathway, which has been implicated in brain ischemia, were identified for association with stroke

Approaches to learning in a classroom environment: observational & experiential

Accreditation Board for Engineering and Technology (ABET) has established a set of criteria that requires engineering graduates to be able to effectively communicate, work in teams with ethics and professionalism understanding the contemporary issues. There are different approaches for teaching and learning these skills, two of which are discussed in this thesis. The intention of this research is to understand the impact of observational and experiential learning on team and individual performance --Abstract, page iv

COVID-19 health crises & justice administration: A case of migrant worker’s plight

India was one of the major countries that implemented the first blockade to counter the COVID 19 pandemic, one of the biggest health crises of this decade, but the blockade still failed. Within a few months, India was the most hit by the coronavirus epidemic. The gap in the Indian government's blockade program highlights the government's mistakes that led to the failure of the blockade in India and continued to expose local migrant workers to intolerable hardships. The manuscript widely violates the fundamental and economic rights granted to local migrant workers and other workers under the Labor Law and the Indian Constitution during the blockade. The national policy during the blockade is for local migrants. It claims to have exacerbated the situation for workers. According to WHO reports first reported in December 2019, COVID-19 is unprecedented, including countless deaths, general economic crisis, unemployment, quarantine, inevitable lockdowns, and a global travel ban. I fell into the whole world with long-term uncertainty. Fighting a pandemic within the population, migrants have emerged as one of the most vulnerable groups in this blockade, as their lives have entirely ceased.&nbsp

Lipopeptides as the Antifungal and Antibacterial Agents: Applications in Food Safety and Therapeutics

A lot of crops are destroyed by the phytopathogens such as fungi, bacteria, and yeast leading to economic losses to the farmers. Members of the Bacillus genus are considered as the factories for the production of biologically active molecules that are potential inhibitors of growth of phytopathogens. Plant diseases constitute an emerging threat to global food security. Many of the currently available antimicrobial agents for agriculture are highly toxic and nonbiodegradable and thus cause extended environmental pollution. Moreover, an increasing number of phytopathogens have developed resistance to antimicrobial agents. The lipopeptides have been tried as potent versatile weapons to deal with a variety of phytopathogens. All the three families of Bacillus lipopeptides, namely, Surfactins, Iturins and Fengycins, have been explored for their antagonistic activities towards a wide range of phytopathogens including bacteria, fungi, and oomycetes. Iturin and Fengycin have antifungal activities, while Surfactin has broad range of potent antibacterial activities and this has also been used as larvicidal agent. Interestingly, lipopeptides being the molecules of biological origin are environmentally acceptable

Physicochemical assessment of industrial textile effluents of Punjab (India)

Abstract Urbanization and industrialization are generating huge quantities of untreated wastewater leading to increased water pollution and human diseases in India. The textile industry is one of the leading polluters of surface water and consumes about 200–270 tons of water to produce 1 ton of textile product. The primary objective of the present study was to investigate the pollution potential of textile industry effluent draining into Buddha Nallah stream located in Ludhiana, Punjab (India), and determine the seasonal variation in physicochemical parameters (pH, water temperature, total dissolved solids, total suspended solids, biochemical oxygen demand (BOD) and chemical oxygen demand (COD) of Buddha Nallah water. During summer months, for Site 1 and Site 2, the value of pH was in the alkaline range of 8.78 ± 0.47 and 8.51 ± 0.41, respectively. The values of pH in the rainy season were found to be in the range of 7.38 ± 0.58 and 7.11 ± 0.59 for Site 1 and Site 2, respectively. In the autumn and winter seasons, the average pH values were found to be in the range of 8.58 ± 1.40 and 8.33 ± 0.970, respectively. The maximum mean temperature in summer was recorded as 41.16 ± 4.99 °C, and lowest mean temperature in winter was recorded as 39.25 ± 2.25 °C at Site 2. The suspended solids were found to be highest (143.5 ± 75.01 and 139.66 ± 71.87 mg/L) in autumn for both the sites and lowest (86.50 + 15.10 mg/L) in the rainy season for Site 1. The values of BOD and COD of the textile effluent of both sites during all the seasons ranged from 121–580 to 240–990 mg/L, respectively, much higher than WHO water quality standard of 30 mg/L for BOD and 250 mg/L for COD. The present study deals with the collection of textile industry effluent and its characterization to find out the physicochemical load being drained by the effluent generated from textile industries, on the natural wastewater streams

Nucleic acid-based aptamers: applications, development and clinical trials

Short single-stranded oligonucleotides called aptamers, often termed as chemical antibodies, have been developed as powerful alternatives to traditional antibodies with respect to their obvious advantages like high specificity and affinity, longer shelf-life, easier manufacturing protocol, freedom to introduce chemical modifications for further improvement, etc. Reiterative selection process of aptamers over 10-15 cycles starting from a large initial pool of random nucleotide sequences renders them with high binding affinity, thereby making them extremely specific for their targets. Aptamer-based detection systems are well investigated and likely to displace primitive detection systems. Aptamer chimeras (combination of aptamers with another aptamer or biomacromolecule or chemical moiety) have the potential activity of both the parent molecules, and thus hold the capability to perform diverse functions at the same time. Owing to their extremely high specificity and lack of immunogenicity or pathogenicity, a number of other aptamers have recently entered clinical trials and have garnered favorable attention from pharmaceutical companies. Promising results from the clinical trials provide new hope to change the conventional style of therapy. Aptamers have attained high therapeutic relevance in a short time as compared to synthetic drugs and/or other modes of therapy. This review follows the various trends in aptamer technology including production, selection, modifications and success in clinical fields. It focusses largely on the various applications of aptamers which mainly depend upon their selection procedures. The review also sheds light on various modifications and chimerizations that have been implemented in order to improve the stability and functioning of the aptamers, including introduction of locked nucleic acids (LNAs). The application of various aptamers in detection systems has been discussed elaborately in order to stress on their role as efficient diagnostic agents. The key aspect of this review is focused on success of aptamers on the basis of their performance in clinical trials for various diseases

Ribonucleases and their Applications

Ribonuclease (RNase) is a type of nuclease that catalyzes degradation of RNA into smaller components. RNase can be classified into two broader categories namely endoribonucleases and exoribonucleases on the basis of their site of action. RNases play key roles in the maturation of all RNA molecules; endoribonucleases cleave the RNA molecules from the interior at 5′ end while exoribonucleases degrade RNA molecules in a 3′–5′ direction. With the advent of new frontiers in biotechnology, the applications of ribonucleases besides molecular biology have expended into many other fields like medicinal, clinical, and analytical chemistry. RNase A that belongs to pancreatic ribonucleases super family plays an important key role in structural, biochemical and evolutionary studies. Discovery of eukaryotic orthologues of the bacterial double stranded (ds) RNA-specific ribonuclease III (RNase III) suggests a central role for these enzymes in the regulation of ds-RNA and eukaryotic RNA metabolism. The more recent studies have shown that the mammalian and some fungal RNases are also bestowed with antiproliferative, antiangiogenic and/ or antitumor/ anticancer activities. Some of the members of RNase A superfamily such as RNase 6 and RNase 7 appears to be evolutionary conserved peptides with potent antimicrobial activities for upkeep of sterility in the urinary tract