Urate Oxidase Purification by Salting-in Crystallization : Towards an Alternative to Chromatography

Background: Rasburicase (FasturtecH or ElitekH, Sanofi-Aventis), the recombinant form of urate oxidase from Aspergillus flavus, is a therapeutic enzyme used to prevent or decrease the high levels of uric acid in blood that can occur as a result of chemotherapy. It is produced by Sanofi-Aventis and currently purified via several standard steps of chromatography. This work explores the feasibility of replacing one or more chromatography steps in the downstream process by a crystallization step. It compares the efficacy of two crystallization techniques that have proven successful on pure urate oxidase, testing them on impure urate oxidase solutions

Molecular markers for plant breeding: comparisons of RFLP and RAPD genotyping costs

Three molecular marker protocols, chemiluminescent restriction fragment length polymorphisms (c-RFLPs), radioactivity-based restriction fragment length polymorphisms (r-RFLPs), and randomly amplified DNA polymorphisms (RAPDs) were compared in terms of cost and time efficiency. Estimates of cost of supplies and time requirements were obtained from simulations of maize (Zea mays L.) genotyping experiments utilizing protocols currently in use. The increase in total cost with increasing numbers of individuals genotyped and markers analyzed is higher for RAPDs than for RFLPs. RAPDs were generally found to be more cost and time efficient for studies involving small sample sizes, while RFLPs have the advantage for larger sample sizes. Because of the shorter exposure times involved, c-RFLPs require less time than r-RFLPs to obtain a given amount of information. Variations in the protocols, such as number of re-uses of Southern blots or cost of Taq DNA polymerase per reaction of amplification, also affect the relative merits of RAPDs and RFLPs. Two examples were analyzed where molecular markers are used: a germ plasm survey and quantitative trait loci (QTL) mapping in a segregating population. No protocol was found to be the most cost and time efficient over the entire range of sample sizes and number of marker loci studied

Cyclooxygenase-2-Derived Prostacyclin Protective Role on Endotoxin-Induced Mouse Cardiomyocyte Mortality

Cardiovascular dysfunction characterizes septic shock, inducing multiple organ failure and a high mortality rate. In the heart, it has been shown an up-regulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions with subsequent overproduction of nitric oxide (NO) and eicosanoids. This study is focused on the links between these products of inflammation and cell loss of mouse cardiomyocytes during treatment by the Salmonella typhimurium lipopolysaccharide (LPS) in presence or in absence of NOS or COX inhibitors. LPS induced RelA/NF-κB p65 activation, iNOS and COX-2 up-regulations, resulting in NO and prostacyclin releases. These effects were reversed by the NO-synthase inhibitor and increased by the specific COX-2 inhibitor. Immunostainings with FITC-conjugated anti-Annexin-V and propidium iodide and caspase 3/7 activity assay showed that cardiomyocyte necrosis was inhibited by L-NA during LPS treatment challenge, while apoptosis was induced in presence of both LPS and NS-398. No effect on LPS cellular injury was observed using the specific cyclooxygenase-1 (COX-1) inhibitor, SC-560. These findings strongly support the hypothesis of a link between iNOS-dependent NO overproduction and LPS-induced cell loss with a selective protective role allotted to COX-2 and deriving prostacyclins

Clarifying European terminology in plastics recycling

The increasing activities in plastics recycling have led to a sprawl of terminology describing different technologies and technology categorizations. This creates not only linguistic confusion but also makes it difficult for regulators, investors, corporate leaders and other stakeholders to fully understand the relationship between different technologies, potentially leading to suboptimal decisions on policy, investment, or collaboration. To bring clarity to this topic, this manuscript provides an overview of (i) the different circular pathways for plastics, with a focus on recycling, (ii) the most common categorization of recycling technologies, (iii) what is considered ‘recycling’ by the European Commission and (iv) some alternative terms used in grey and academic literature to describe recycling technologies

Ab initio calculations of the hydrogen bond

Recent x-ray Compton scattering experiments in ice have provided useful information about the quantum nature of the interaction between H$_2$O monomers. The hydrogen bond is characterized by a certain amount of charge transfer which could be determined in a Compton experiment. We use ab-initio simulations to investigate the hydrogen bond in H$_2$O structures by calculating the Compton profile and related quantities in three different systems, namely the water dimer, a cluster containing 12 water molecules and the ice crystal. We show how to extract estimates of the charge transfer from the Compton profiles.Comment: 16 pages, 7 figures, to appear in Phys. Rev.

Novel robotic assistive technologies: choosing appropriate training for healthcare professionals

One of the key challenges for the training of healthcare professionals (HCPs) is to maintain a good understanding and knowledge of new assistive technologies (ATs) that are currently on the market [1]. Indeed, at present, available training on ATs is limited and does not meet the practice-related needs of HCPs. It is in this context that the ADAPT European project aims to develop a new AT training programme for healthcare professionals, which will also introduce them to the project’s new AT developments - a smart powered wheelchair and a virtual reality wheelchair-driving simulator. The program consists of six multimodal units; five delivered via e-learning and one through a blended method of e-learning and face-to-face sessions. The development of the content is guided by findings from an earlier literature review and an online survey of AT training needs for HCP’s, both undertaken by the ADAPT cross-national research team, comprised of UK and French experts. The level of technical difficulty increases with successive units in order to train all HCPs to use innovative ATs more widely in their practice. A Learning Management System enables the dissemination of the e-learning AT program. Preliminary results from participant unit-specific evaluations available at this stage are overall positive and encouraging

LINEARISATION D’UN MODELE DE REACTEUR A BOUES ACTIVEES

Cet article présente une stratégie de linéarisation d’un modèle non linéaire de réacteur à boues activées. Cette stratégie a pour but d’obtenir un modèle linéaire simplifié, pour des fins d’estimation, de commande et d’analyse de stabilité. En appuyant sur un modèle initiale comportant trois variables d’états représentant respectivement : la concentration du substrat biodégradable, la concentration de la biomasse hétérotrophe et la concentration de l’oxygène dissous, une approche systématique de réduction de complexité du modèle est envisagée. Cette approche comprend essentiellement trois étapes. Dans la première étape, une analyse de sensibilité est effectuée dont le but est d’hiérarchiser les variables d’états selon leurs effets sur la réponse du modèle. Par la suite, une procédure de linéarisation est appliquée, basée sur une combinaison linéaire pondérée des termes présentant des non-linéarités dans le système. Les variables identifiées comme non influentes peuvent être ignorées, le modèle s’en trouve avantageusement simplifié. Dans la dernière étape, les paramètres inconnus des termes linéaires trouvés sont identifiés en utilisant un algorithme évolutionnaire. L’avantage principal de la méthode proposée est de conserver la structure générale ainsi que l’ordre du modèle original

Angular versus radial correlation effects on momentum distributions of light two-electron ions

We investigate different correlation mechanisms for two-electron systems and compare their respective effects on various electron distributions. The simplicity of the wave functions used allows for the derivation of closed-form analytical expressions for all electron distributions. Among other features, it is shown that angular and radial correlation mechanisms have opposite effects on Compton profiles at small momenta.Comment: 22 pages, 5 figures, 3 tabl

Turbulence and particle acceleration in collisionless supernovae remnant shocks: II- Cosmic-ray transport

Supernovae remnant shock waves could be at the origin of cosmic rays up to energies in excess of the knee ($E\simeq3\cdot 10^{15}$eV) if the magnetic field is efficiently amplified by the streaming of accelerated particles in the shock precursor. This paper follows up on a previous paper \citep{pell05} which derived the properties of the MHD turbulence so generated, in particular its anisotropic character, its amplitude and its spectrum. In the present paper, we calculate the diffusion coefficients, also accounting for compression through the shock, and show that the predicted three-dimensional turbulence spectrum $k_\perp S_{3\rm d}(k_\parallel,k_\perp)\propto k_\parallel^{-1}k_\perp^{-\alpha}$ (with $k_\parallel$ and $k_\perp$ the wavenumber components along and perpendicular to the shock normal) generally leads to Bohm diffusion in the parallel direction. However, if the anisotropy is constrained by a relation of the form $k_\parallel \propto k_\perp^{2/3}$, which arises when the turbulent energy cascade occurs at a constant rate independent of scale, then the diffusion coefficient loses its Bohm scaling and scales as in isotropic Kolmogorov turbulence. We show that these diffusion coefficients allow to account for X-ray observations of supernova remnants. This paper also calculates the modification of the Fermi cycle due to the energy lost by cosmic rays in generating upstream turbulence and the concomittant steepening of the energy spectrum. Finally we confirm that cosmic rays can produced an amplified turbulence in young SNr during their free expansion phase such that the maximal energy is close to the knee and the spectral index is close to 2.3 in the warm phase of the interstellar mediumComment: 13 pages, 4 figures, accepted for publication in Astronomy & Astrophysics main journa

A refined model for spinning dust radiation

We present a comprehensive treatment of the spectrum of electric dipole emission from spinning dust grains, updating the commonly used model of Draine and Lazarian. Grain angular velocity distributions are computed using the Fokker-Planck equation; we revisit the drift and diffusion coefficients for the major torques on the grain, including collisions, grain-plasma interactions, and infrared emission. We use updated grain optical properties and size distributions. The theoretical formalism is implemented in the companion code, SPDUST, which is publicly available. The effect of some environmental and grain parameters on the emissivity is shown and analysed.Comment: Minor corrections. Matches the MNRAS published version (except for a typo in Eq.(74) corrected here). The companion code, SPDUST, can be downloaded from http://www.tapir.caltech.edu/~yacine/spdust/spdust.htm