Molecular Characterization of Macrophomina phaseolina, the Incitant of Coleus forskohlii Revealed by RAPD Markers

Coleus forskohlii belong to family lamiaceae is one of the commercial plants grown extensively in the country, the chemical found in the Coleus which has both medicinal application and gives great economy to the industrial organizations. Unfortunately, these plants are being highly succumbed to serious diseases like wilt and root rot caused by a fungus, hence the growers and industrialists are facing severe problem in safeguarding this crop in the field irrespective of the agro climatic conditions. Root rot disease, is one of the major diseases of Coleus forskohlii which, is caused by Macrophomina phaseolina , Pathogen variability was studied at both morphological and molecular level using cultural characteristics and Rapid Amplification of Polymorphic DNA (RAPD) analysis respectively. Totally thirty two isolates were isolated from roots of Coleus forskohlii . In RAPD 165 bands were obtained out of them 121 bands (73.3%) were polymorphic with a similarity coefficient of 0.48-0.66. Clusters analysis of RAPD data when Unweighted Pair Group Method with Arithmetic Mean (UPGMA) Tree constructed using NTSYS, it showed 6 groups. Among them two were major clusters and 4 were minor clusters with similarity coefficient 0.48-0.66. The pathogenicity of the isolates was tested on Coleus forskohlii plants. Analysis of the pathogenicity tests results revealed that the isolates grouped under two major clusters which were different from the one obtained using RAPD data. The results indicate that the data from RAPD analysis and Pathogenicity tests do not correlate with each other

Biochemical and minerals studies of K-134 variety groundnut (Arachis hypogaea. L) grown in municipal water

Sewage water is the result of urbanization, which contain organic and inorganic materials, which effect water quality and surrounding ecosystem. Discharge of untreated sewage water into near water bodies is so common. In Mysore (India) about 70% of population depends on agriculture and the farmers are at the mercy of seasonal, irregular and inadequate rainfall. So the farmers in urban areas of Mysore irrigating the sewage water for their agriculture lands for enhancing productivity of ground nut crop which is the common practice for many years. However the impact of such massive pollution on plant growth and metabolism is of considerable interest. Municipal waste water contains organic and inorganic compounds including macronutrents like N.P. and K (Mara and Coirn Cross, 1998). In India many works studied the effect of sewage water on various agricultural crops like Srivastava and Singh (1990) on Abelmosehus esculentus, Devi (1991) on Coriander, and Fenugreek, Singh and Singh (1992) on Lactuca sativa, Shanmugavel (1993) on greengram and maize, Srivastava et al., (1993) on Amaranthus ssp. Nashikkar (1994) on Wheat and maize, Sharma et al., (1997) on Urd bean, Kannabiran and Harilal (1998) on Vigna mungo , Krishna and Viyaya Kumar (2000) on Adenanthera pavonina, Suresh Babu et al., (2000) on Oryza sativa, Augusthy and Mani (2001) on Vigna radiatus, Pradhan etal. (2001) on black gram, and Anil , Mosmi (2005) on Cicer arietinum and Nagajyoti et al., (2008) on ground nut. The present investigation was carried out to study the effect of untreated sewage water on mineral and biochemical content on different varieties of groundnut at different concentration