Tetrazolium reduction by guard cells in abaxial epidermis of Vicia faba: blue light stimulation of a plasmalemma redox system

The stomata in the abaxial epidermis of Vicia faba were examined for the location of redox systems using tetrazolium salts. Three distinct redox systems could be demonstrated: chloroplast, mitochondrial, and plasmalemma. The chloroplast activity required light and NADP. Mitochondnal activity required added NADH and was suppressed by preincubation with KCN. The plasmalemma redox system in guard cells also required NADH, but was insensitive to KCN and was stimulated by blue light. The involvement of an NADH dehydrogenase in the blue light stimulated redox system in guard cells was suggested by the sensitivity to plantanetin, an inhibitor of NADH dehydrogenase. The redox system of mitochondria was the most active followed by that of plasmalemma. The activity of chloroplasts was the least among the three redox systems. The plasmalemma mediated tetrazolium reduction was stimulated by exogenous flavins and suppressed by KI or phenylacetate, inhibitors of flavin excitation. We therefore conclude that an NADH-dependent, flavin mediated electron transport system, sensitive to blue light, operates in the plasmalemma of guard cells

Structure of Complexes of 2-Phenylazopyridine with Perchlorate Halides of Ni(II) & with Ferrous Iodide

166-16

A screening of the dicotyledonous weed flora for the occurrence of C<SUB>4</SUB> dicarboxylic acid pathway of photosynthesis

Using the criteria of leaf anatomy, carbon dioxide compensation point and photosynthetic rate under reduced oxygen tension, a number of the local herbaceous and dicotyledonous weeds were screened for the occurrence of the C4 pathway of photosynthesis. Of the thirty-two species, belonging to nine families seventeen species revealed the characteristics of the C4 plants. All of the plants surveyed here were hitherto not tested and therefore the present results extend the number of plants already known to possess the Hatch-Slack pathway of photosynthesis. Another noteworthy feature of the investigation was the finding of interspecific differences within the generaHeliotropium, Alternanthera, Mollugo andEuphorbia in the presence or absence of the C4 pathway

Shifts in photosynthetic carbon labelling pattern by etiolated rice (Oryza sativa L.) seedlings during greening

The carbon assimilation pattern in light by dark-grown rice (Oryza sativa) seedlings was studied during greening. After exposure of etiolated seedlings to light their capacity to synthesize the C4-acids, malate and aspartate, increased for 12 h. The labelling of 3-phosphoglycerate, sugar phosphates, sucrose and insolubles, did not increase until after 12 h. Thereafter a continuous steep increase in synthesis of Calvin cycle intermediates, but not of C4-acids, recurred. The levels of carboxylating enzymes (phosphoenolpyruvate and ribulose diphosphate carboxylases) in the seedlings correlated positively with the appearance of label in C4 acids and in Calvin cycle compounds. We suggest that a &#946;-carboxylation mechanism was activated immediately on illumination but was persistent for only 12 h. After an 8-h lag period following illumination, the Calvin cycle began to operate and continued during further growth of seedlings in light

Bilarge neutrino mixing from supersymmetry with high-scale nonrenormalizable interactions

We suggest a supersymmetric (SUSY) explanation of neutrino masses and mixing, where nonrenormalizable interactions in the hidden sector generate lepton number violating Majorana mass terms for both right-chiral sneutrinos and neutrinos. It is found necessary to start with a superpotential including an array of gauge singlet chiral superfields. This leads to nondiagonal $\Delta L = 2$ mass terms and almost diagonal SUSY breaking $A$-terms. As a result, the observed pattern of bilarge mixing can be naturally explained by the simultaneous existence of the seesaw mechanism and radiatively induced masses. Allowed ranges of parameters in the gauge singlet sector are delineated, corresponding to each of the cases of normal hierarchy, inverted hierarchy and degenerate neutrinos.Comment: 19 pages, 5 figures. Minor modifications are made in the title and the text, some new references are added. To appear in this form in Physical Review

Essentiality of mitochondrial oxidative metabolism for photosynthesis: optimization of carbon assimilation and protection against photoinhibition

The review emphasizes the essentiality of mitochondrial oxidative metabolism for photosynthetic carbon assimilation. Photosynthetic activity in chloroplasts and oxidative metabolism in mitochondria interact with each other and stimulate their activities. During light, the partially modified TCA cycle supplies oxoglutarate to cytosol and chloroplasts. The marked stimulation of O2 uptake after few minutes of photosynthetic activity, termed as light enhanced dark respiration (LEDR), is now a well-known phenomenon. Both the cytochrome and alternative pathways of mitochondrial electron transport are important in such interactions. The function of chloroplast is optimized by the complementary nature of mitochondrial metabolism in multiple ways: facilitation of export of excess reduced equivalents from chloroplasts, shortening of photosynthetic induction, maintenance of photorespiratory activity, and supply of ATP for sucrose biosynthesis as well as other cytosolic needs. Further, the mitochondrial oxidative electron transport and phosphorylation also protects chloroplasts against photoinhibition. Besides mitochondrial respiration, reducing equivalents (and ATP) are used for other metabolic phenomena, such as sulfur or nitrogen metabolism and photorespiration. These reactions often involve peroxisomes and cytosol. The beneficial interaction between chloroplasts and mitochondria therefore extends invariably to also peroxisomes and cytosol. While the interorganelle exchange of metabolites is the known basis of such interaction, further experiments are warranted to identify other biochemical signals between them. The uses of techniques such as on-line mass spectrometric measurement, novel mutants/transgenics, and variability in metabolism by growth conditions hold a high promise to help the plant biologist to understand this interesting topic

Superconductivity in Ru substituted BaFe2-xRuxAs2

The occurrence of bulk superconductivity at ~22 K is reported in polycrystalline samples of BaFe2-xRuxAs2 for nominal Ru content in the range of x=0.75 to 1.125. A systematic suppression of the spin density wave transition temperature (TSDW) precedes the appearance of superconductivity in the system. A phase diagram is proposed based on the measured TSDW and superconducting transition temperature (TC) variations as a function of Ru composition. Band structure calculations, indicate introduction of electron carriers in the system upon Ru substitutiom. The calculated magnetic moment on Fe shows a minimum at x=1.0, suggesting that the suppression of the magnetic moment is associated with the emergence of superconductivity. Results of low temperature and high field Mossbauer measurements are presented. These indicate weakening of magnetic interaction with Ru substitutionComment: 20 pages 10 figure

Evaluation of Hexane Extract of Tuber of Root of Cyperus rotundus Linn (Cyperaceae) for Repellency against Mosquito Vectors

Hexane extract of tuber of plant Cyperus rotundus (Cyperaceae) was screened under laboratory conditions for repellent activity against mosquito vector Anopheles culicifacies Giles species A (Diptera: Culicidae), Anopheles stephensi Liston (Diptera: Culicidae), and Culex quinquefasciatus Say (Diptera: Culicidae). The Cyperus rotundus tuber extract was used to determine their effect on mosquito vector, and comparison with the DEET (NN Diethyl 1-3 methyl Benzamide, formerly known as diethyl 1-m-toluamide). The tuber extracts showed more effective at all the dose. Result obtained from the laboratory experiment showed that the tuber extracts are more effective for repellency of allthe mosquito vector even at low dose. Clear dose response relationships were established with the highest dose of 10% tuber extract evoking 100% repellency. Percent protection obtained against An. culicifacies Giles species A 100% repellency in 4 hours, 6 hours, An. stephensi 100% repellency in 6 hours and Cx. quinquefasciatus was 100% repellency in 6 hours at the 10% concentration. Against DEET- 2.5% An. culicifacies A 100% repellency in 1 hour, 2 hours, 6 hours, An. stephensi have shown 100% repellency in 6 hours, and Culex quinquefasciatus have shown 100% repellency in 1 hour, 2 hours, 6 hours. The consolidated data of the repellency observed in different species is given and it is evident that the over all repellency rates varied between 80 and 100% for different repellents concentrations (2.5%, 5%, and 10%). The extract can be applied as an effective personal protective measure against mosquito bites

New Binuclear Nickel(II) Species

785-78

Supergravity can reconcile dark matter with lepton number violating neutrino masses

Supersymmetry offers a cold dark matter candidate, provided that lepton number is {\em not violated by an odd number of units}. On the other hand, lepton number violation by even (two) units gives us an attractive mechanism of neutrino mass generation. Here we offer an explanation of this, in a supergravity framework underlying a supersymmetric scenario, the essential feature being particles carrying lepton numbers, which interact only gravitationally with all other known particles. It is shown that one can have the right amount of $\Delta L = 2$ effect giving rise to neutrino masses, whereas the lifetime for $\Delta L = 1$ decays of the lightest supersymmetric particle can be prolonged beyond the present age of the universe.Comment: 8 pages, significant changes in the conceptual ideas and presentation of the paper, minor chages in the title and abstract, added few references. This is to appear in Physical Review