Organic slug control using Phasmarhabditis hermaphrodita

Phasmarhabditis hermaphrodita is a lethal slug parasitic nematode that has been formulated into an effective biological control agent called Nemaslug®. We investigated the possibility of using different application methods of P. hermaphrodita to reduce cost and the number of nematodes applied. We also compared P. hermaphrodita with a new slug pellet called Ferramol®, which is available for use on organic farms

Microscale Mechanics of Plug-and-Play In Vitro Cytoskeleton Networks

This chapter describes recent techniques that have been developed to reconstitute and characterize well-controlled, tunable networks of actin and microtubules outside of cells. It describes optical tweezers microrheology techniques to characterize the linear and nonlinear mechanics of these plug-and-play in vitro networks from the molecular-level to mesoscopic scales. It also details fluorescence microscopy and single-molecule tracking methods to determine macromolecular transport properties and stress propagation through cytoskeleton networks. Throughout the chapter the intriguing results that this body of work has revealed are highlighted—including how the macromolecular constituents of cytoskeleton networks map to their signature responses to stress or strain; and the elegant couplings between network structure, macromolecular mobility, and stress response that cytoskeleton networks exhibit

Polymer threadings and rigidity dictate the viscoelasticity and nonlinear relaxation dynamics of entangled ring-linear blends and their composites with rigid rod microtubules

Mixtures of polymers of varying topologies and stiffnesses display complex emergent rheological properties that often cannot be predicted from their single-component counterparts. For example, entangled blends of ring and linear polymers have been shown to exhibit enhanced shear thinning and viscosity, as well as prolonged relaxation timescales, compared to pure solutions of rings or linear chains. These emergent properties arise in part from the synergistic threading of rings by linear polymers. Topology has also been shown to play an important role in composites of flexible (e.g., DNA) and stiff (e.g., microtubules) polymers, whereby rings promote mixing while linear polymers induce de-mixing and flocculation of stiff polymers, with these topology-dependent interactions giving rise to highly distinct rheological signatures. To shed light on these intriguing phenomena, we use optical tweezers microrheology to measure the linear and nonlinear rheological properties of entangled ring-linear DNA blends and their composites with rigid microtubules. We show that the linear viscoelasticity is primarily dictated by the microtubules at lower frequencies, but their contributions become frozen out at frequencies above the DNA entanglement rate. In the nonlinear regime, we reveal that mechanical response features, such as shear thinning, stress softening and multi-modal relaxation dynamics are mediated by entropic stretching, threading, and flow alignment of entangled DNA, as well as forced de-threading, disentanglement, and clustering. The contributions of each of these mechanisms depend on the strain rate as well as the entanglement density and stiffness of the polymers, leading to non-monotonic rate dependences of mechanical properties that are most pronounced for highly concentrated ring-linear blends rather than DNA-MT composites.Comment: 22 pages, 8 figure

OpTiDDM (Optical Tweezers integrating Differential Dynamic Microscopy) maps the spatiotemporal propagation of nonlinear stresses in polymer blends and composites

How local stresses propagate through polymeric fluids, and, more generally, how macromolecular dynamics give rise to viscoelasticity are open questions vital to wide-ranging scientific and industrial fields. Here, to unambiguously connect polymer dynamics to force response, and map stress propagation in macromolecular materials, we present a powerful approach-Optical Tweezers integrating Differential Dynamic Microscopy (OpTiDMM)-that simultaneously imposes local strains, measures resistive forces, and analyzes the motion of the surrounding polymers. Our measurements with blends of ring and linear polymers (DNA) and their composites with stiff polymers (microtubules) uncover a surprising resonant response, in which affine alignment, superdiffusivity, and elastic memory are maximized when the strain rate is comparable to the entanglement rate. Microtubules suppress this resonance, while substantially increasing elastic force and memory, due to varying degrees to which the polymers buildup, stretch and flow along the strain path, and configurationally dissipate stress. More broadly, the rich multi-scale coupling of mechanics and dynamics afforded by OpTiDDM, empowers its interdisciplinary use to elucidate non-trivial phenomena that sculpt stress propagation dynamics-critical to commercial applications and cell mechanics alike.Comment: 32 pages, 10 figure

Direct measurement of the intermolecular forces confining a single molecule in an entangled polymer solution

We use optical tweezers to directly measure the intermolecular forces acting on a single polymer imposed by surrounding entangled polymers (115 kbp DNA, 1 mg/ml). A tube-like confining field was measured in accord with the key assumption of reptation models. A time-dependent harmonic potential opposed transverse displacement, in accord with recent simulation findings. A tube radius of 0.8 microns was determined, close to the predicted value (0.5 microns). Three relaxation modes (~0.4, 5 and 30 s) were measured following transverse displacement, consistent with predicted relaxation mechanisms.Comment: 11 pages, 3 figure

Active restructuring of cytoskeleton composites leads to increased mechanical stiffness, memory, and heterogeneity

The composite cytoskeleton, comprising interacting networks of semiflexible actin and rigid microtubules, actively generates forces and restructures using motor proteins such as myosins to enable key mechanical processes including cell motility and mitosis. Yet, how motor-driven activity alters the mechanics of cytoskeleton composites remains an open challenge. Here, we perform optical tweezers microrheology on actin-microtubule composites driven by myosin II motors to show that motor activity increases the linear viscoelasticity and elastic storage of the composite by active restructuring to a network of tightly-packed filament clusters and bundles. Our nonlinear microrheology measurements performed hours after cessation of activity show that the motor-contracted structure is stable and robust to nonlinear forcing. Unique features of the nonlinear response include increased mechanical stiffness, memory and heterogeneity, coupled with suppressed filament bending following motor-driven restructuring. Our results shed important new light onto the interplay between viscoelasticity and non-equilibrium dynamics in active polymer composites such as the cytoskeleton