17 research outputs found
Significance of molecular diagnostics in human papilloma virus (HPV) determination
HPV infection is considered to be the most important etiologic factor in
cervical cancer development. In this retrospective study, which included the
period from 2000 to 2012, the results of two molecular techniques used in
the detection of HPV infection among women of the South Bačka District were
analyzed. By using the technique of in situ hybridization and the rPCR
method, the proportion of high-risk HPV among women with normal cytology was
determined to be 19.8% and 32.7%, respectively, and among women with
abnormal cytology 43.1% and 61%, respectively. Among the analyzed women, HPV
type 16 was the most prevalent, followed by HPV types 31, 51 and 18.
Application of molecular HPV diagnosis is valuable because it increases the
sensitivity of the screening test, so that the application of both tests to
detect cervical cancer is a true prevention of malignancy
Influenza A and B viruses in the population of Vojvodina, Serbia
At present, two influenza A viruses, H1N1pdm09 and H3N2, along with influenza
B virus co-circulate in the human population, causing endemic and seasonal
epidemic acute febrile respiratory infections, sometimes with
life-threatening complications. Detection of influenza viruses in
nasopharyngeal swab samples was done by real-time RT-PCR. There were 60.2%
(53/88) positive samples in 2010/11, 63.4% (52/82) in 2011/12, and 49.9%
(184/369) in 2012/13. Among the positive patients, influenza A viruses were
predominant during the first two seasons, while influenza B type was more
active during 2012/13. Subtyping of influenza A positive samples revealed the
presence of A (H1N1)pdm09 in 2010/11, A (H3N2) in 2011/12, while in 2012/13,
both subtypes were detected. The highest seroprevalence against influenza A
was in the age-group 30-64, and against influenza B in adults aged 30-64 and
>65. [Projekat Ministarstva nauke Republike Srbije, br. TR31084
Prevalence of oncogenic Human papillomavirus and genetic diversity in the L1 gene of HPV16 HPV 18 HPV31 and HPV33 found in women from Vojvodina Province Serbia.
The main purpose of this paper is to estimate the pre-vaccination prevalence of 12 hrHPV types among 564 women from Vojvodina province (Serbia). The corrected contingency coefficient (Ccorr) was used to estimate the importance of association of examined HPV types and cytological diagnosis. The highest association with the abnormal cytology was observed for HPV 16 (Ccorr = 0.493) in all age groups of participants. The effect of HPV 16 was especially clear within the group of women older than 35 years (Ccorr = 0.691), compared with women younger than 35 (Ccorr = 0.333). The molecular characterization at the level of L1 gene of HPV 16, 18, 31 and 33 variants was for the first time assessed in our region. Nearly all HPV 16 isolates cluster with variant lineage A (96.4%) the remaining isolates clustering with variant lineage D. All of HPV 18 and HPV 33 isolates are clustering within the lineage A while isolates of HPV 31 group with lineages A and C. This contributes to understanding of intrinsic geographical and biological differences of examined HPV types and could be useful for development of cervical cancer screening strategies in Vojvodina (Serbia) and diagnosis of HPV related cervical cancer in general
Viral gastrointestinal syndrome in our environment
Viral gastrointestinal syndrome is a cause of morbidity and death worldwide.
Infection is spread through contact with an infected person, as well as
through contaminated food and water. A lethal outcome is possible in infants
and young children due to dehydration and electrolyte imbalance. The study
included 141 patients with gastroenteritis from Vojvodina. Real-Time PCR
method in stool samples was used to determine the presence of rota-, noro-,
and astrovirus nucleic acid. Out of 141 patients with gastroenteritis, 60.3%
were confirmed to have one of the three viruses. Rotavirus was significantly
more common in children up to 3 years of age (43.3%). Norovirus was more
frequently detected in patients older than 20 (50%). These infections started
in collectives. Astrovirus was detected in four patients (2.8%). The results
confirm the necessity to implement PCR in routine diagnostics for the proper
treatment of patients
West Nile virus infection in humans and other vertebrates
The West Nile virus is an arthropod borne or ARBO virus from the Flaviviridae
family, which is maintained in nature in the transmission cycle between
hosting birds and ornithophilic mosquito vectors. The virus is capable of
infecting different vertebrate species and 60 mosquito species. The infection
in humans can be asymptomatic or it can have different clinical
manifestations ranging from light febrile diseases to fatal
meningoencephalitis. This paper presents recent findings on the activity of
the West Nile virus in Europe, the USA and Serbia. Presented are the results
of serological testing of human populations and animals in Serbia, and the
methods of molecular diagnostics to prove the existence of the virus.
[Projekat Ministarstva nauke Republike Srbije, br. TR31084 i br. TR43007