A Functional Gene Array for Detection of Bacterial Virulence Elements

Emerging known and unknown pathogens create profound threats to public health. Platforms for rapid detection and characterization of microbial agents are critically needed to prevent and respond to disease outbreaks. Available detection technologies cannot provide broad functional information about known or novel organisms. As a step toward developing such a system, we have produced and tested a series of high-density functional gene arrays to detect elements of virulence and antibiotic resistance mechanisms. Our first generation array targets genes from Escherichia coli strains K12 and CFT073, Enterococcus faecalis and Staphylococcus aureus. We determined optimal probe design parameters for gene family detection and discrimination. When tested with organisms at varying phylogenetic distances from the four target strains, the array detected orthologs for the majority of targeted gene families present in bacteria belonging to the same taxonomic family. In combination with whole-genome amplification, the array detects femtogram concentrations of purified DNA, either spiked in to an aerosol sample background, or in combinations from one or more of the four target organisms. This is the first report of a high density NimbleGen microarray system targeting microbial antibiotic resistance and virulence mechanisms. By targeting virulence gene families as well as genes unique to specific biothreat agents, these arrays will provide important data about the pathogenic potential and drug resistance profiles of unknown organisms in environmental samples

College student's academic goals and learning strategies

Tomando en cuenta que no existe concordancia en la literatura entre los resultados de diferentes estudios respecto de la relevancia de la adopción de un tipo u otro de meta para el aprendizaje académico, en el presente artículo se estudia la relación entre los diferentes tipos de orientaciones motivacionales (metas de aprendizaje, metas de rendimiento y metas de evitación del trabajo) y el uso de estrategias cognitivas y de autorregulación. En el trabajo participan 632 estudiantes universitarios de diversas titulaciones de la universidad española. Aunque el estudio es de tipo correlacional, los análisis de los datos obtenidos, corroborando estudios anteriores, aportan información de interés para la discusión del problema que se investigó, por ejemplo que sólo los niveles más altos de metas de aprendizaje se encuentran asociados con una mayor utilización de estrategias de aprendizaje. Son discutidas implicaciones educativas de estos datos.Tomando em consideração que não existe concordância na literatura entre os resultados dos diferentes estudos a respeito da relevância da adopção de um ou de outro tipo de meta na aprendizagem académica; no presente artículo é estudada a relação entre os diferentes tipos de orientações motivacionais (metas de aprendizagem, metas de rendimento e metas de evitação do trabalho) e a utilização de estratégias cognitivas e de autoregulação. Neste trabalho participam 632 estudantes universitários de diversas licenciaturas da Universidade espanhola. Apesar de este estudo ser de tipo correlacional, a análise dos dados obtidos, corroborando estudos anteriores, aporta informação de interesse para a discussão do problema investigado; por exemplo que só os níveles mais altos de metas de aprendizagem se encontram associados con uma maior utilização de estratégias de aprendizagem. São discutidas implicações educativas destes dados.Assuming that there isn’t much agreement among the results from different studies relating to the relevance of adopting one or another kind of academic learning goal, this paper intends to study the relationship between the several types of motivational orientations (learning goals, achievement goals and avoidance goals) and the use of cognitive and self-regulatory strategies. 632 Spanish college students from various university courses participated in this research. Although this is a correlacional study data analysis, in line with previous studies, brings to light interesting information and highlights the investigated issue for example only higher levels of learning goals are positively related with the use of learning strategies. Future educational implications are also discussed

Evaluation of a Web Portal for Improving Public Access to Evidence-Based Health Information and Health Literacy Skills: A Pragmatic Trial

Background: Using the conceptual framework of shared decision-making and evidence-based practice, a web portal was developed to serve as a generic (non disease-specific) tailored intervention to improve the lay public’s health literacy skills. Objective: To evaluate the effects of the web portal compared to no intervention in a real-life setting. Methods: A pragmatic randomised controlled parallel trial using simple randomisation of 96 parents who had children aged ,4 years. Parents were allocated to receive either access to the portal or no intervention, and assigned three tasks to perform over a three-week period. These included a searching task, a critical appraisal task, and reporting on perceptions about participation. Data were collected from March through June 2011. Results: Use of the web portal was found to improve attitudes towards searching for health information. This variable was identified as the most important predictor of intention to search in both samples. Participants considered the web portal to have good usability, usefulness, and credibility. The intervention group showed slight increases in the use of evidencebased information, critical appraisal skills, and participation compared to the group receiving no intervention, but these differences were not statistically significant. Conclusion: Despite the fact that the study was underpowered, we found that the web portal may have a positive effect on attitudes towards searching for health information. Furthermore, participants considered the web portal to be a relevant tool. It is important to continue experimenting with web-based resources in order to increase user participation in health care decision-making. Trial Registration: ClinicalTrials.gov NCT0126679