Errata Corrige: Stress-Induced Phosphorylation of C-Jun-N-Terminal Kinases and Nuclear Translocation of Hsp70 in the Wistar Rat Hippocampus (Vol 61, Pg 1, 2009)

In the paper entitled: Adžić, M., Đorđević, A., Krstić-Demonacos, M., & Radojčić, M. B. (2009). Stress-induced phosphorylation of c-Jun-N-terminal kinases and nuclear translocation of Hsp70 in the Wistar rat hippocampus. Archives of Biological Sciences, 61(1), 1-8. Fig. 1, on page 4, section b, should read "Nucleus" instead of "Cytoplasm

Carbonized Jute Sorbent for Oil Cleanup

Over 90 million tons of textile waste is produced every year. A large share of waste comes from the goods made of cellulose fibers. Recently, special attention has been directed towards the use of textile cellulose waste for clean-up of oil spills. The major problem relies on their relatively small oil capacity and complex separation of individual cellulose fibers from the treated spills. In an attempt to overcome this drawback, a non-woven sorbent based on recycled jute fibers obtained from the carpet industry was manufactured. Improvement of porosity and hydrophobicity/oleophilicity of the sorbent was achieved by carbonization process in an inert atmosphere. FESEM analysis revealed the fiber reduction of almost 40% induced by fiber degradation while EDX analysis confirmed the increase in the carbon content by 75% after carbonization. Oil capacity in water medium, buoyancy, oil retention and reusability of non-carbonized and carbonized sorbents were evaluated by testing four different oils (crude oil, diesel oil, two motor oils). After carbonization process, the oil sorption capacity was doubled in comparison with noncarbonized sorbent independent of oil viscosity. Carbonized sorbent not only remained afloat after 24 h of staying in water, but it sorbed a negligible amount of water unlike non-carbonized sorbent. in addition to good buoyancy, oil retention on carbonized sorbents ranged from 64-80% after 30 min of draining. Larger uptake was achieved with oils of higher viscosity, but their retention was worse. Oil sorption capacity after 5 repeated sorption/desorption trials was significantly larger in the case of carbonized sorbent since it retained 80-88% of its initial oil sorption capacity depending on tested oil

Independent associations of childhood and current socioeconomic status with risk of self-reported doctor-diagnosed arthritis in a family-medicine cohort of North-Carolinians

Most experimental models, including cell culture studies, have demonstrated that over-expression of manganese superoxide dismutase (MnSOD) in cells bearing a carcinoma phenotype has anti-proliferative and tumour suppression characteristics. In contrast, when cervical carcinoma biopsies express MnSOD, there is a poor prognosis and resistance to radiation therapy. The results herein indicate that human cervical adenocarcinoma (HeLaS3) cells have increased MnSOD activity (up to 50 % of the total SOD activity) due to low expression of its repressor p53 and a high level of oxidative stress arising from the cell culture conditions. High MnSOD activity may be related to I-IcLaS3 cell radioresistance. illustrated by a high IC50 of 3.4 Gy and by a relatively high level of cell viability after gamma irradiation. In contrast to MnSOD activity, cytosolic CuZnSOD activity decreased after ionising radiation. The catalase (Cat) activity was unchanged. IR also increased the nitric oxide synthase (NOS) activity. Such conditions lead to increased concentrations of the superoxide radical, hydrogen peroxide and NO, which together may be responsible for the decreased expression of NF-B-K and unaltered Cat activity. Therefore, the disturbed redox balance within HeLaS3 cells may be responsible for the cytotoxicity observed at higher irradiation doses. It could be concluded that inhibition of the CuZrISOD activity may be an important target for the selective killing of radioresistant cancer cells

Synthesis and characterization of Co2+ doped TiO2 nanoparticles

Scrolled titania nanotubes were synthesized by a hydrothermal method using commercial TiO2 powder in proton-deficient aqueous systems. In presence of CoCl2 extended hydrothermal treatment of TiO2 nanotubes lead to formation Co2+ doped TiO2 nanoparticles. TEM and HRTEM measurement were used for determining the shape, dimension and structure of doped nanoaprticles. XRD confirmed anatase crystalline structure od Co2+ doped TiO2. The presence of Co2+ ions did not induce distortions of the overall anatase crystal structure of TiO2 matrix. The concentration of Co2+ ions was 1.5% compared with the amount of Ti4+ ions.Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 24-28 September 200

Bottom-up creation of multifunctional textile nano-entities using TiO2/Ag nanocrystals

The 11th International Conference on Modern Manufacturing Technologies in Industrial Engineering 2023 (ModTech 2023) / hybrid edition, June 14 - 17, Bucharest, Romania, 2023.Invited speaker (Invitation lecture)

Gamma-radiation induced agglomeration of chicken muscle myosin and actin

Radiolytic behaviour of the major vertebrate muscle proteins: fibril Jar myosin (rnolar mass, M-m = 520,000 g/mol) and filament fortning actin (M-m = 42,050 g/mol) was studied using a SDS-polyacrylamide gel electrophoresis and quantified by high precision laser-densitometry. In order to study the OH radical contribution to the radiation damage, purified chicken myosin and actin (4 muM) were prepared in N2O saturated solution and irradiated with 1-3 kGy at Co-60 gamma source. With respect to changes in the molecular mass, the only observed myosin and actin damage was dose dependent agglomeration of proteins. The corresponding radiation chemical yields of 5 x 10(-8) mol J(-1) and 6.3 x 10(-8) mol J(-1) were obtained for myosin and actin, respectively. This result confirmed that only the radiation-induced agglomeration is initiated with the reaction of the OH radical even in the situation where the OH radical concentration produced exceeds the protein concentration 500 times, thus enabling the multi-radical attack to occur

Gamma-radiation induced damage of proteins in the thick fraction of egg white

The thick fraction of egg white saturated with either N2O or Ar was irradiated in the dose range 1.5-45 kGy at Co-60 gamma source. The gel structure decomposition and other processes accompanied with changes in protein molecular mass were followed by Sephadex G-200 exclusion chromatography, denaturing SDS-polyacrylamide gel electrophoresis, viscosity and turbidity measurements. The complex behaviour of viscosity was observed in the NO saturated sample (where the hydrated electron was converted into the OH radical); the initial abrupt decrease that gradually slows down reaching the minimum at 12 kGy (eta(mm) = 2.7 mPa s) followed by the slow rise was measured. The Ar saturated sample ([e(aq)(-)] approximate to [OH]) showed both the significantly faster initial decrease and lower viscosity minimum (eta(min) = 2.2 mPa s). The combined Sephadex G-200 exclusion chromatography and denaturing SDS-polyacrylamide gel electrophoresis data revealed that the three-dimensional egg white (hydrated) gel structure was (efficiently) decomposed even in the N2O saturated sample. The protein scission was detected in the entire dose range studied, while the protein agglomeration is not noticed at low doses (around 1.5 kGy); however, it dominates at higher doses. In the highest dose region studied, the loss of structure in SDS-PAGE chromatograms indicates that the agglomerates arc formed from protein fragments rather than from intact proteins. The continuous linear increase in turbidity was measured. The results obtained indicate that ionizing radiation causes the breakdown of the protein network of the thick fraction of egg white via the reduction of S S bridges by the hydrated electron and the protein fragmentation due to the direct action of ionizing radiation. The protein agglomeration is initiated by the reaction of the OH radical; its inefficiency at low doses is attributed to the glucose antioxidant properties and radical immobility

Synthesis, characterization and photocatalytic activity of anatase nanoparticles doped with gadolinium ions

Gd3+-doped anatase nanoparticles have been synthesized via acidic hydrolysis of Gd3+ and Ti4+ organic precursors. The resulting products were characterized by Xray diffraction (XRD) and diffuse reflection spectra (DRS). Experimental results indicated that different Gd3+-doping levels make great impact on the optical properties and photocatalytic activity of the obtained TiO2 nanoparticles. All applied doping concentrations induced enhancement of photoactivity regarding degradation of Rhodamine B (RB), compared to pure TiO2. The inter-band defect states formed as a result of incorporation of Gd3+ ions in the TiO2 matrix are most probably the cause of observed improved photoacatalytic activity and optical properties of the doped TiO2 nanoparticles.Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 24-28 September 201

Novel TiO2/Ag/TiO2 cotton-based nanocomposites for wastewater treatment

The 7th International Conference on the Characterization and Control of Interfaces for High Quality Advanced Materials and the 57th Summer Symposium on Powder Technology 2022 (ICCCI 2022), November 15 - 18, Fujiyoshida, Japan, 2022.Invited speaker (Invitation Lecture)

Stress-Induced Phosphorylation of C-Jun-N-Terminal Kinases and Nuclear Translocation of Hsp70 in the Wistar Rat Hippocampus

Glucocorticoids are key regulators of the neuroendocrine stress response in the hippocampus. Their action is partly mediated through the subfamily of MAPKs termed c-jun-N-terminal kinases (JNKs), whose activation correlates with neurodegeneration. The stress response also involves activation of cell protective mechanisms through various heat shock proteins (HSPs) that mediate neuroprotection. We followed both JNKs and Hsp70 signals in the cytoplasmic and nuclear compartments of the hippocampus of Wistar male rats exposed to acute, chronic, and combined stress. The activity of JNK1 was decreased in both compartments by all three types of stress, while the activity of cytoplasmic JNK2/3 was elevated in acute and unaltered or lowered in chronic and combined stress. Under all stress conditions, Hsp70 translocation to the nucleus was markedly increased. The results suggest that neurodegenerative signaling of JNKs may be counteracted by increase of nuclear Hsp70, especially under chronic stress