T Cell Mediated Antibody lnvariance in an Immune Response Against A Bacterial Carbohydrate Antigen Requires CD28/B7–1 Costimulation

The humoral immune response against α(1→3) dextran (Dex) in BALB/c mice is characterized by the formation of predominantly IgM antibodies bearing the J558 idiotype. IgG antibodies do not appear in euthymic mice. In athymic animals however, the response proceeds to a vigorous IgG production. In euthymic mice formation of IgG is suppressed by J558 idiotype- specific regulatory T cells recognizing in association with I-Ed and in cognate T/B interaction the VH CDR3 derived peptide of the J558 idiotpye. Only B-2 lymphocytes produce IgG whereas B-1 cells do not participate in the production of this Ig class. Using a novel synthetic all α(1→3)-D-gluco configurated tetrasaccharide the Dex-specific B cells can for the first time be analyzed in FACS. In experiments using this newly designed low molecular Dex no signs of B cell apoptosis can be found. This demonstrates a true silencing of persisting Bγ memory cells and supports previous by adoptive transfer experiments. In this suppression an involvement of CD28/B7–1 interaction can be demonstrated which is a necessary costimulatory suppression signal in addition to the cognate TCR/peptide-I-Ed interaction between J558 Id-specific T cells and J558 idiotype beating B cells. This results in an activation of 178–4 Ts cells, leading to an overall suppression of the Dex-specific IgG isotype production on the one hand and on the other hand provides a signal for the survival and clonal expansion of J558 Id-positive B cells

Měnová politika a cena ropy

The article discusses the relationship between monetary policy and price of oil, in broader sense price of commodities. Firstly it focuses on describing the relationship of key macroeconomic variables, gas prices and other commodities against oil prices. Subsequently, it discusses the existence of a „transmission channels“ through which monetary policy can be propagated into oil prices (or prices of commodities). Secondly it provides further insight into the forecasting process of the CNB, in both a retrospective look back at the prospects of oil prices in the past and the analysis of transitory and permanent shock (the rise in oil prices of 30 USD/b). Simulated oil price shock is calculated from the average level of Brent oil prices in the fi rst quarter of 2010, i.e. 77.50 USD/b.Web of Science591462

Energy Efficiency and GHG Emissions: Prospective Scenarios for the Aluminium Industry

This study examines the possibilities for energy efficiency and GHG emission improvements in the European aluminium industry. The first part of the study presents the status quo of the industry in the EU28 and Iceland by compiling a database of existing plants with their production characteristics and the best available and innovative technologies (BATs/ITs). A model EU is then developed to simulate the trend in each plant towards 2050. The use of the model in different scenarios allows the analysis of the cost-effectiveness of investments in BATs/ITs. The results show that in absolute terms, for the whole industry the energy consumption and direct GHG emissions can decrease from 2010 to 2050 by 21% and 66%, respectively. And, in almost all scenarios, for the primary aluminium production there is a convergence in the reduction of specific energy consumption and direct GHG emissions of 23% and 72%, respectively. Since most of the savings come from technologies that are in early stages of research, there is a clear need of a decided push and of creating the right conditions to make these potential savings happen.JRC.F.6-Energy Technology Policy Outloo

Bacterial diversity in typical abandoned multi-contaminated nonferrous metal(loid) tailings during natural attenuation

This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this recordAbandoned nonferrous metal(loid) tailings sites are anthropogenic, and represent unique and extreme ecological niches for microbial communities. Tailings contain elevated and toxic content of metal(loid)s that had negative effects on local human health and regional ecosystems. Microbial communities in these typical tailings undergoing natural attenuation are often very poorly examined. The diversity and inferred functions of bacterial communities were examined at seven nonferrous metal(loid) tailings sites in Guangxi (China), which were abandoned between 3 and 31 years ago. The acidity of the tailings sites rose over 31 years of site inactivity. Desulfurivibrio, which were always coupled with sulfur/sulfide oxidation to dissimilate the reduction of nitrate/nitrite, were specific in tailings with 3 years abandonment. However, genus beneficial to plant growth (Rhizobium), and iron/sulfur- oxidizing bacteria and metal(loid)-related genera (Acidiferrobacter and Acidithiobacillus) were specific within tailings abandoned for 23 years or more. The increased abundance of acid-generating iron/sulfur-oxidizing and metal(loid)-related bacteria and specific bacterial communities during the natural attenuation could provide new insights for understanding microbial ecosystem functioning in mine tailings. OTUs related to Sulfuriferula, Bacillus, Sulfurifustis, Gaiella, and Thiobacillus genera were the main contributors differentiating the bacterial communities between the different tailing sites. Multiple correlation analyses between bacterial communities and geochemical parameters indicated that pH, TOC, TN, As, Pb, and Cu were the main drivers influencing the bacterial community structures. PICRUSt functional exploration revealed that the main functions were related to DNA repair and recombination, important functions for bacterial adaptation to cope with the multi- contamination of tailings. Such information provides new insights to guide future metagenomic studies for the identification of key functions beyond metal- transformation/resistance. As well, our results offers novel outlooks for the management of bacterial communities during natural attenuation of multi-contaminated nonferrous metal(loid) tailings sites.International Key Project from National Natural Science Foundation of ChinaProjects of Natural Science Foundation of ChinaPublic welfare project of Chinese Ministry of Environmental Protectionnternational key project of Ministry of Science and Technology of ChinaS2016G2135Centre National de la Recherche ScientifiqueRoyal Society Newton Mobility GrantNational Natural Science Foundation International Joint collaboration China-Swede

European energy poverty metrics:Scales, prospects and limits

ENGAGER 2017–2021, CA16232Energy poverty, a condition whereby people cannot secure adequate home energy services, is gaining prominence in public discourse and on political and policy agendas. As its measurement is operationalised, metrical developments are being socially shaped. A European Union mandate for biennial reporting on energy poverty presents an opportunity to institutionalise new metrics and thus privilege certain measurements as standards. While combining indicators at multiple scales is desirable to measure multi-dimensional aspects, it entails challenges such as database availability, coverage and limited disaggregated resolution. This article converges scholarship on metrics e which problematises the act of measurement e and on energy poverty e which apprehends socio-political and techno-economic particulars. Scholarship on metrics suggests that any basket of indicators risks silencing significant but hard to measure aspects, or unwarrantedly privileging others. State-of-the-art energy poverty scholarship calls for indicators that represent contextualised energy use issues, including energy access and quality, expenditure in relation to income, built environment related aspects and thermal comfort levels, while retaining simplicity and comparability for policy traction. We frame energy poverty metrology as the socially shaped measurement of a varied, multi-dimensional phenomenon within historically bureaucratic and publicly distant energy sectors, and assess the risks and opportunities that must be negotiated. To generate actionable knowledge, we propose an analytical framework with five dimensions of energy poverty metrology, and illustrate it using multi-scalar cases from three European countries. Dimensions include historical trajectories, data flattening, contextualised identification, new representation and policy uptake. We argue that the measurement of energy poverty must be informed by the politics of data and scale in order to institutionalise emerging metrics, while safeguarding against their co-optation for purposes other than the deep and rapid alleviation of energy poverty. This ‘dimensioned’ understanding of metrology can provide leverage to push for decisive action to address the structural underpinnings of domestic energy deprivation.publishersversionpublishe

Strengths-Weaknesses-Opportunities-Threats analysis of carbon footprint indicator and derived recommendations

ABSTRACT: Demand for a low carbon footprint may be a key factor in stimulating innovation, while prompting politicians to promote sustainable consumption. However, the variety of methodological approaches and techniques used to quantify life-cycle emissions prevents their successful and widespread implementation. This study aims to offer recommendations for researchers, policymakers and practitioners seeking to achieve a more consistent approach for carbon footprint analysis. This assessment is made on the basis of a comprehensive Strengths-Weaknesses-Opportunities-Threats or SWOT Analysis of the carbon footprint indicator. It is carried out bringing together the collective experience from the Carbonfeel Project following the Delphi technique principles. The results include the detailed SWOT Analysis from which specific recommendations to cope with the threats and the weaknesses are identified. In particular, results highlight the importance of the integrated approach to combine organizational and product carbon footprinting in order to achieve a more standardized and consistent approach. These recommendations can therefore serve to pave the way for the development of new, specific and highly-detailed guidelines

Impacts of adaptation and responsibility framings on attitudes towards climate change mitigation

It is likely that climate change communications and media coverage will increasingly stress the importance of adaptation, yet little is known about whether or how this may affect attitudes towards mitigation. Despite concerns that communicating adaptation could undermine public support for mitigation, previous research has found it can have the opposite effect by increasing risk salience. It is also unclear whether people respond differently to information about mitigation and adaptation depending on whether action is framed as an individual or government responsibility. Using an experimental design, this study sought to examine how public attitudes towards mitigation are influenced by varying climate change messages, and how this might interact with prior attitudes to climate change. UK-based participants (N = 800) read one of four texts in a 2 × 2 design comparing adaptation versus mitigation information and personal versus governmental action. No main effect was found for adaptation versus mitigation framing, nor for individual action versus government policy, but we did observe a series of interaction effects with prior attitudes to climate change. Mitigation and adaptation information affected participants’ responses differently depending on their pre-existing levels of concern about climate change, suggesting that mitigation framings may be more engaging for those with high levels of concern, whereas adaptation framings may be more engaging for low-concern individuals. Government mitigation action appears to engender particularly polarised attitudes according to prior concern. Implications for climate change communications are considered