M\u27Dor L\u27Dor- From Generation to Generation: The Jewish Legacy in Cleveland

Leatrice Rabinsky discusses the transmission of Jewish heritage and traditions from generation to generation and how this has contributed to the continuity of Judaism. The author highlights the growth and development of Cleveland’s Jewish community and the establishment of communal institutions and traditions within the community. Conference paper; originally published in Western Reserve Studies Symposium (9th:1994 : Cleveland, Ohio

Effect of Protein Coatings on the Delivery Performance of Liposomes.

Modifications of the surface properties of liposomal drug carriers, such as coating with therapeutic or targeting proteins, greatly impact their delivery performance. Many of the currently-used methods to associate proteins with the surface of the liposomes can be laborious, inefficient, and significantly change the delivery characteristics. In this dissertation, two relatively simple methods are explored for their ability to associate proteins with the liposome membrane and their impact on the liposome’s delivery properties. The first method that is characterized is the non-covalent conjugation of a polyhistidine (His)-tagged protein to the surface of liposomes containing nickel-chelating lipid. It is shown that the His-tagged model protein, yellow fluorescent protein (YFP-His), associates with the liposome membrane without impacting the stability or uptake properties of liposomes. Next, the effect of the liposome surface-associated protein antigen on the performance of a liposomal vaccine carrier that has previously shown to be efficient in inducing both cellular and humoral immune responses was tested. Not only was the cellular immune response uncompromised by this new liposome formulation, but also the humoral immune response to encapsulated antigen was enhanced. Thus, this study shows that additional antigens can be non-covalently coupled to the liposome membrane in this manner without impacting the delivery properties of the encapsulated antigen and while enhancing the overall immunogenicity of the vaccine. The second method investigated is the association of recombinant amphiphilic protein with the liposomal membrane via hydrophobic interaction. A truncated form of the amphiphilic protein apolipoprotein B (apoB), apoB23, was fused to YFP and used to associate YFP with the liposome membrane. The apoB23-YFP fusion protein did not affect the liposome’s properties in vitro. An apoB23 fusion protein was then prepared with a HER2-specific antibody fragment and used to coat the liposome membranes. The density at which apoB23 fusion proteins coated the liposome membrane, however, was low and no significant selectivity for HER2-overexpressing cells was observed with apoB23-αHER2-coated liposomes. Despite this, it was demonstrated that the apoB fusion protein could effectively associate with the liposome membrane without affecting the liposome’s characteristics and is suggestive of its utility in other targeting applications.Ph.D.Pharmaceutical SciencesUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue.lib.umich.edu/bitstream/2027.42/86502/1/efr_1.pd

Philosophy of journalism: On the theory and practice of the news enterprise

The aim of this dissertation is to examine critically the nature of journalism and to address philosophical issues in the theory and practice of journalism.I present three arguments in support of the claim that a libertarian framework is preferable to other frameworks: (1) a Lockean argument, defending the conceptual connection between \u27freedom of the press\u27 and the journalist\u27s \u27right to private property\u27 (this argument is designed to show that a libertarian model of the press is ultimately the most defensible, on ethical grounds); (2) a \u27Wider Range of Truths\u27 argument, designed to show that there is more possibility for journalists to report on a wider range of truths within a libertarian media system than in other socio-political models of the press; and (3) an Instrumental argument, designed to show that \u27good journalism\u27---the creation of objective, veridical reports---requires that journalists be legally free to use their property (their news organizations, news gathering equipment, etc.) as they choose. (And as long as others\u27 rights are not violated.)Ultimately, the journalist\u27s primary responsibility is to tell the truth : i.e., to create objective, veridical reports about events in the world. In doing so, journalists fulfill a unique and valuable function in society. (Abstract shortened by UMI.