1 research outputs found
Evaluation of CD9 Expression of Tumour Cells and Stromal Immune Cells in Breast Carcinoma by Immunohistochemistry
Introduction: Cluster of Differentiation 9 (CD9), known as
Motility Related Protein (MRP-1) regulates cell adhesion,
motility, migration and proliferation. Many studies have stated
conflicting results on prognostic significance of invasive breast
carcinomas with CD9 expression that had performed on tumour
tissues.
Aim: To assess the inter-relationship of CD9 expression of
tumour cells and stromal immune cells in breast carcinoma
with clinicopathological parameters which include age, tumour
size, grade, histological type, lymph nodes, tumour staging and
molecular classification.
Materials and Methods: An observational prospective (July
2020 to June 2021) and retrospective (October 2019 to June 2020)
study was done in 71 cases of resected primary invasive breast
carcinoma over a period of one and half year at Sri Devaraj Urs
Medical College, Karnataka, India. Immunohistochemistry (IHC)
staining was done using CD9 antibody. Tumour cells (T-CD9)
expression was evaluated by Immunoreactivity scoring (IS=
Intensity score×Extent of staining). The stromal cells (S-CD9)
expression was evaluated by percentage (%) of stromal area
occupied by CD9 stained immune cells. Chi-square test was
used as test of significance for qualitative data. The p-value of
<0.05 was considered as statistically significant.
Results: Out of 71 cases, T-CD9 expression was noticed in 40
(56.34%) cases and IS <4 considered as negative was observed
in 31 (43.66%) cases. However there was no association
with age, tumour size, grade and molecular markers for the
expression of both T-CD9 and S-CD9. Human Epidermal growth
factor receptor 2 neu (HER2neu) negative was associated with
T-CD9 expression (p-value=0.05). Hence, CD9 can be used as
prognostic marker for Her2neu negative cases.
Conclusion: The CD9 expression was not significantly
associated with tumour cells (T-CD9) and stromal cells (S-CD9)
in breast carcinoma cases. However, it was significantly
associated with Her2neu negative tumour cells. T-CD9 showed
more positivity in Luminal A followed by triple negative, whereas
S-CD9 showed more positivity in Luminal B. CD9 did not show
association with any parameters except Her2neu negative