Generation of the first BAC-based physical map of the common carp genome

<p>Abstract</p> <p>Background</p> <p>Common carp (<it>Cyprinus carpio</it>), a member of Cyprinidae, is the third most important aquaculture species in the world with an annual global production of 3.4 million metric tons, accounting for nearly 14% of the all freshwater aquaculture production in the world. Apparently genomic resources are needed for this species in order to study its performance and production traits. In spite of much progress, no physical maps have been available for common carp. The objective of this project was to generate a BAC-based physical map using fluorescent restriction fingerprinting.</p> <p>Result</p> <p>The first generation of common carp physical map was constructed using four- color High Information Content Fingerprinting (HICF). A total of 72,158 BAC clones were analyzed that generated 67,493 valid fingerprints (5.5 × genome coverage). These BAC clones were assembled into 3,696 contigs with the average length of 476 kb and a N50 length of 688 kb, representing approximately 1.76 Gb of the common carp genome. The largest contig contained 171 BAC clones with the physical length of 3.12 Mb. There are 761 contigs longer than the N50, and these contigs should be the most useful resource for future integrations with linkage map and whole genome sequence assembly. The common carp physical map is available at <url>http://genomics.cafs.ac.cn/fpc/WebAGCoL/Carp/WebFPC/</url>.</p> <p>Conclusion</p> <p>The reported common carp physical map is the first physical map of the common carp genome. It should be a valuable genome resource facilitating whole genome sequence assembly and characterization of position-based genes important for aquaculture traits.</p

The Robust Control and Synchronization of a Class of Fractional-Order Chaotic Systems with External Disturbances via a Single Output

This paper investigates the stabilization and synchronization of a class of fractional-order chaotic systems which are affected by external disturbances. The chaotic systems are assumed that only a single output can be used to design the controller. In order to design the proper controller, some observer systems are proposed. By using the observer systems some sufficient conditions for achieving chaos control and synchronization of fractional-order chaotic systems are derived. Numerical examples are presented by taking the fractional-order generalized Lorenz chaotic system as an example to show the feasibility and validity of the proposed method

Robust Exponential Synchronization of a Class of Chaotic Systems with Variable Convergence Rates via the Saturation Control

This article is concerned with the exponential synchronization of a class of the chaotic systems with external disturbance via the saturation control. Through appropriate coordinate transformation, the exponential synchronization is translated into the asymptotic stability of the error system. By using the Lyapunov stability theory, a novel sufficient condition which possesses the exponential convergence rate λ is presented. The rich choices of the exponential convergence rate λ turn our scheme more general than some existing approaches. Numerical simulations are employed to the Genesio chaotic system and the Coullet chaotic system to illustrate the ability and effectiveness of the presented approach

A HYBRID TWO-STAGE FLOWSHOP SCHEDULING PROBLEM

This paper considers a batch scheduling problem in a two-stage hybrid flowshop that consists of m dedicated parallel machines in stage 1 and a batch processor in stage 2. The processing time of a batch is defined as the largest processing time of the jobs contained in that batch. The criterion is to minimize the makespan, the time by which all operations of jobs have been processed. For such a problem, we present a polynomial time algorithm for the case with all jobs having the same processing time on the batch processor. An approximation algorithm with a competitive ratio 2 for the general case is also presented.Scheduling, flexible flowshop, dedicated machines, batch processor

A Novel Fast Convergence Control Scheme for a Class of 3D Chaotic Systems with Uncertain Parameters and External Disturbances

This paper studies the control of a class of 3D chaotic systems with uncertain parameters and external disturbances. A new method which is referred as the analytical solution approach is firstly proposed for constructing Lyapunov function. Then, for suppressing the trajectories of the 3D chaotic system to its equilibrium point 00,0,0, a novel fast convergence controller containing parameter λ which determines the convergence rate of the system is presented. By using the designed Lyapunov function, the stability of the closed-loop system is proved via the Lyapunov stability theorem. Computer simulations are employed to a new chaotic system to illustrate the effectiveness of the theoretical results

Forecasting the Incidence of Mumps in Zibo City Based on a SARIMA Model

This study aimed to predict the incidence of mumps using a seasonal autoregressive integrated moving average (SARIMA) model, and provide theoretical evidence for early warning prevention and control in Zibo City, Shandong Province, China. Monthly mumps data from Zibo City gathered between 2005 and 2013 were used as a training set to construct a SARIMA model, and the monthly mumps in 2014 were defined as a test set for the model. From 2005 to 2014, a total of 8722 cases of mumps were reported in Zibo City; the male-to-female ratio of cases was 1.85:1, the age group of 1–20 years old accounted for 94.05% of all reported cases, and students made up the largest proportion (65.89%). The main serious endemic areas of mumps were located in Huantai County, Linzi District, and Boshan District of Zibo City. There were two epidemic peaks from April to July and from October to January in next year. The fitted model SARIMA (0, 1, 1) (0, 1, 1)12 was established (AIC = 157.528), which has high validity and reasonability. The SARIMA model fitted dynamic changes of mumps in Zibo City well. It can be used for short-term forecasting and early warning of mumps

Adverse Events in Treating Smear-Positive Tuberculosis Patients in China

This study aimed to estimate the adverse events (AE) rate during anti-tuberculosis treatment and to explore AE-related risk factors. New and previously treated smear-positive tuberculosis (TB) cases were enrolled from eight regions in China between April 2009 and October 2010. The AE rate was estimated, and AE risk factors during anti-TB treatment were assessed using Cox proportional models. Among 2091 Chinese subjects with anti-TB treatment, 462 (22.1%, 95% confidence interval (CI), 20.3–23.9) patients developed AE, with liver injury and gastrointestinal reactions constituting the most common AE. Specifically, 9.8% (95% CI, 8.5–11.1) and 6.3% (95% CI, 5.3–7.4) developed liver injuries and gastrointestinal reactions, respectively. We found that AE rate differed by regions, TB knowledge score, symptoms score and smoking status. Liver injuries were associated with age, sex and smoking status; gastrointestinal reactions were associated with education level and symptom score. Improving patients’ knowledge on TB could reduce AE rate