25 research outputs found
Aerosol climatology in the northwestern Indian Himalaya: a study based on the radiative properties of aerosol
Characteristics of atmospheric aerosol particles and their role in aerosol radiative forcing over the northwestern Indian Himalaya in particular and over India in general
Not Available
Not AvailableSYBR green based RT-qPCR assay coupled with melting curve analysis was developed for the detection of
Tomato leaf curl New Delhi virus-potato (ToLCNDV-[potato]), Potato virus X (PVX), and Potato leafroll virus (PLRV).
The assays were standardized using four sets of primers for each target and selecting best primer set, optimizing primer
concentration and cycle conditions. The amplification of products were verified by specific melting peaks for each targets
viz., ToLCNDV-[potato], PLRV, PVX and ef-1 α gene of potato. Then, duplex RT-qPCR assays were developed to detect these
viruses along with elongation factor 1-α (ef-1 α) gene as plant internal control and to detect ToLCNDV-[potato] along with
PVX and PLRV. In duplex RT-qPCR assay, a melting peak specific to ToLCNDV-[potato] was observed along with melting
peak of other virus/ ef-1 α gene. The assays could detect up to 20 copies/μl using serially diluted plasmids harbouring the
targets and up to 0.025 fg of total RNA from infected plant tissues. Sensitivity of duplex RT-qPCR assay was comparable
to singleplex RT-qPCR assays. The developed assays could consistently detect these viruses in field samples and was more
sensitive than conventional RT-PCR.Not Availabl