22 research outputs found
Luminal ions and short chain fatty acids as markers of functional activity of the mucosa in ulcerative colitis.
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Nitric oxide-dependent nitrosation of cellular CoA: A proposal for tissue responses
No full textCopyright © 2001 Academic Press. All rights reserved.W.E.W. Roedige
Methionine derivatives diminish sulphide damage to colonocytes--implications for ulcerative colitis.
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Selective inhibition of fatty acid oxidation in colonocytes by ibuprofen: a cause of colitis?
No full textThiol methyltransferase activity in colonocytes and erythrocyte membranes.
No full textAimsTo verify the improved thiol methyltransferase (TMT) assay and measure activity in isolated colonocytes and erythrocyte membranes of the same subjects.MethodsHigh performance liquid chromatography with radioactivity detection was used to measure 14C-methylmercaptoethanol formation, the reaction product of cell extracts incubated with mercaptoethanol and 14C-S-adenosylmethionine.ResultsVerification of radiolabelled 14C-methylmercaptoethanol was by exogenous addition of methylmercaptoethanol and simultaneous ultraviolet detection at 214 nm. Using a substrate concentration of 10 mM mercaptoethanol, the Km for S-adenosylmethionine was 25 microM. The sensitivity of the radioactive method was 2 pmol, with coefficients of variation of 7% within assay and 6.4% between assay. TMT activities (mean +/- SE; n = 17) were 471 +/- 64 pmol/hour/mg protein for colonocytes and 73 +/- 7 pmol/hour/mg protein for erythrocyte membranes.ConclusionsThe direct assay of TMT activity is sensitive, specific and eliminates concern over non-enzymatic methylation of thiol compounds. High activities in colonic epithelial cells deserve evaluation in disease states
Effects of oral parenteral nutrition solution on the morphology and mechanical resistance of the small bowel in rats
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