Production of Specific Fragments of {varphi}X174 Replicative Form DNA by a Restriction Enzyme from Haemophilus parainfluenzae, Endonuclease HP

A restriction endonuclease from Haemophilus parainfluenzae degrades {varphi}X174 replicative form DNA into eight specific fragments, ranging from 1,700 to 150 base pairs and terminated specifically by deoxycytidylic acid

Determination of transmitter function by neuronal activity

The role of neuronal activity in the determination of transmitter function was studied in cultures of dissociated sympathetic neurons from newborn rat superior cervical ganglia. Cholinergic and adrenergic differentiation were assayed by incubating the cultures with radioactive choline and tyrosine and determining the rate of synthesis and accumulation of labelled acetylcholine and catecholamines. As in previous studies, pure neuronal cultures grown in control medium displayed much lower ratios of acetylcholine synthesis to catecholamine synthesis than did sister cultures grown in medium previously conditioned by incubation on appropriate nonneuronal cells (conditioned medium). However, here we report that neurons treated with the depolarizing agents elevated K+ or veratridine, or stimulated directly with electrical current, either before or during application of conditioned medium, displayed up to 300-fold lower acetylcholine/catecholamine ratios than they would have without depolarization, and thus remained primarily adrenergic. Elevated K+ and veratridine produced this effect on cholinergic differentiation without significantly altering neuronal survival. Because depolarization causes Ca2+ entry in a number of cell types, the effects of several Ca2+ agonists and antagonists were investigated. In the presence of the Ca2+ antagonists D600 or Mg2+, K+ did not prevent the induction of cholinergic properties by conditioned medium. Thus depolarization, either steady or accompanying activity, is one of the factors determining whether cultured sympathetic neurons become adrenergic or cholinergic, and this effect may be mediated by Ca2+

Switching and Extension of a [c2]Daisy-Chain Dimer Polymer

We report the synthesis of a [c2]daisy-chain dimer via ruthenium-catalyzed ring-closing olefin metathesis. Confirmation of the interlocked nature of the structure was achieved through single-crystal X-ray diffraction analysis. The dimer could be readily switched from the bound to the unbound conformation by treatment with 3.0 equiv of KOH and subsequently reprotonated by treatment with 3.0 equiv of HPF_6. Azide functionalization of the dimer enabled incorporation in linear step-growth polymer chains using the alkyne-azide “click” reaction. Gel permeation chromatography coupled with multiangle laser light scattering analysis showed the polymers contained 22 dimers and had a radius of gyration of 14.8 nm. Acylation of the amines of the dimers sterically forced elongation of the interlocked units, and MALLS analysis of the polymer showed a 48% increase in the R_g (21.4 nm)

Effects of bubbles on the electrochemical behavior of hydrogen-evolving Si microwire arrays oriented against gravity

The size-distribution, coverage, electrochemical impedance, and mass-transport properties of H₂ gas-bubble films were measured for both planar and microwire-array platinized n⁺-Si cathodes performing the hydrogen-evolution reaction in 0.50 M H₂SO₄ (aq). Inverted, planar n⁺-Si/Ti/Pt cathodes produced large, stationary bubbles which contributed to substantial increases in ohmic potential drops. In contrast, regardless of orientation, microwire array n⁺-Si/Ti/Pt cathodes exhibited a smaller layer of bubbles on the surface, and the formation of bubbles did not substantially increase the steady-state overpotential for H₂ (g) production. Experiments using an electroactive tracer species indicated that even when oriented against gravity, bubbles enhanced mass transport at the electrode surface. Microconvection due to growing and coalescing bubbles dominated effects due to macroconvection of gliding bubbles on Si microwire array cathodes. Electrodes that maintained a large number of small bubbles on the surface simultaneously exhibited low concentrations of dissolved hydrogen and small ohmic potential drops, thus exhibiting the lowest steady-state overpotentials. The results indicate that microstructured electrodes can operate acceptably for unassisted solar-driven water splitting in the absence of external convection and can function regardless of the orientation of the electrode with respect to the gravitational force vector

Pseudo-rip: Cosmological models intermediate between the cosmological constant and the little rip

If we assume that the cosmic energy density will remain constant or strictly increase in the future, then the possible fates for the universe can be divided into four categories based on the time asymptotics of the Hubble parameter H(t): the cosmological constant, for which H(t) = constant, the big rip, for which H(t) goes to infinity at finite time, the little rip, for which H(t) goes to infinity as time goes to infinity, and the pseudo-rip, for which H(t) goes to a constant as time goes to infinity. Here we examine the last of these possibilities in more detail. We provide models that exemplify the pseudo-rip, which is an intermediate case between the cosmological constant and the little rip. Structure disintegration in the pseudo-rip depends on the model parameters. We show that pseudo-rip models for which the density and Hubble parameter increase monotonically can produce an inertial force which does not increase monotonically, but instead peaks at a particular future time and then decreases.Comment: 4 pages, 2 figures, title changed to agree with published versio

Evolution of Neuroimaging Technology in the Modern Era

Clinical applications in brain science have progressed at a glacial pace when compared to other medical disciplines. Treatments for most neurodegenerative brain diseases are limited, and cure strategies remain underdeveloped. Pressure to improve clinical outcomes in the neurological sciences is exacerbated by an aging population at risk for degenerative brain diseases. Fortunately, technical advances in the field of neuroimaging offer new promise, with enhanced characterization of microstructural anatomy, network connectivity, and functional biomarkers of health and disease. Articles highlighted in this issue describe cutting-edge applications targeting these outcomes using diffusion tensor imaging, diffusion-based tractography, and positron emission tomography. Finally, the glymphatic system is reviewed as a target for future neuroimaging investigation in clinical populations such as those with Alzheimer\u27s disease. Integration of these methods with new advances in computational science will inform mechanisms of healthy and dysfunctional brain mechanisms and ideally lead to new targeted therapeutic interventions