11 research outputs found
Monoclonal-Antibodies Which Inhibit Invitro Cytotoxicity of Axenic Entamoeba-Histolytica
No full textA panel of 12 independent hybridoma cell lines secreting monoclonal antibodies to axenic E. histolytica (HM1) have been developed. A hybridoma cell line P4 C4 P2 F8 C8 (clone C8) produced monoclonal antibodies (MoAb C8) of IgG1 isotype which recognised a 29 KD surface associated antigen of amoebic trophozoites in Western immunoblot. Immunofluorescent probing with MoAb C8 employing live and acetone fixed amoebic trophozoites indicated 29 KD molecule on the surface plasma membrane of E. histolytica trophozoites. The MoAb C8 also agglutinated the live amoebic trophozoites. Pretreatment of amoebic trophozoites with anti 29 KD monoclonal antibody significantly (P < 0.01) inhibited in vitro cytotoxicity of amoebic trophozoites to the cultured baby hamster kidney (BHK-21) cells. MoAb recognised a 29 KD molecule of E. histolytica trophozoites which mediated cytotoxic potentials of the parasite. The absence or variable degree of expression of cytotoxic 29 KD molecule may possibly serve as a marker to differentiate virulent/avirulent populations or strains of E. histolytica
Uses and Limitations in the Demonstration of Specific Circulating Immune-Complexes in Patients with Amebiasis
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Combining Ability for Foliar Resistance to Late Blight [Phytophthora infestans (Mont.) de Bary] of Potato Cultivars with Different Levels of Resistance
No full textComputational pharmacokinetics/pharmacodynamics of rifampin in a mouse tuberculosis infection model
No full textSexual introgression of the late blight resistance gene Rpi-blb3 from a Mexican wild diploid species Solanum pinnatisectum Dunal into potato varieties
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