18 research outputs found
Expression At The Cell-Surface Of Native Fusion Protein Of The Newcastle-Disease Virus (Ndv) Strain Italien From Cloned Cdna
A cDNA library was constructed with poly(A)+-mRNAs from NDV-Italien infected
BHK-21 cells. A clone, that hybridized to the F gene mRNA, was sequenced. A long
open reading frame encodes for a protein of 553 amino acids, with a calculated
molecular weight of 59,153, consisting of twelve cysteine residues and six
potential glycosylation sites. The protein sequence contains a hydrophobic region
at the N-terminus of F1 and a presumptive long transmembrane fragment near the
C-terminus. Comparison of the F proteins from NDV strains Italien and
Australia-Victoria shows that the sequences are very similar, with conservation
of most cysteine residues and of the potential glycosylation sites. The F coding
sequence was inserted into the genome of vaccinia virus under the control of
vaccinia P7.5 transcriptional regulatory sequences. Expression of F protein was
demonstrated by indirect immunofluorescence with five anti-F monoclonal
antibodies known to react with conformational epitopes
Caveolin targeting to late endosome/lysosomal membranes is induced by perturbations of lysosomal pH and cholesterol content
Caveolin-1 traffics to late endosomal/lysosomal membranes in response to manipulations of the cholesterol content of cells, suggesting that caveolin functions in the egress of cholesterol from this organelle. Cavicles associate with the periphery of the lysosome as they do with caveosomes, but these are separate organelles