Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing

Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample

Frequency-specific flow control in microfluidic circuits with passive elastomeric features

A fundamental challenge in the design of microfluidic devices lies in the need to control the transport of fluid according to complex patterns in space and time, and with sufficient accuracy. Although strategies based on externally actuated valves have enabled marked breakthroughs in chip-based analysis1, 2, 3, 4, 5, this requires significant off-chip hardware, such as vacuum pumps and switching solenoids, which strongly tethers such devices to laboratory environments6, 7, 8, 9, 10. Severing the microfluidic chip from this off-chip hardware would enable a new generation of devices that place the power of microfluidics in a broader range of disciplines. For example, complete on-chip flow control would empower highly portable microfluidic tools for diagnostics, forensics, environmental analysis and food safety, and be particularly useful in field settings where infrastructure is limited. Here, we demonstrate an elegantly simple strategy for flow control: fluidic networks with embedded deformable features are shown to transport fluid selectively in response to the frequency of a time-modulated pressure source. Distinct fluidic flow patterns are activated through the dynamic control of a single pressure input, akin to the analog responses of passive electrical circuits composed of resistors, capacitors and diodes<br/