Not Available

Not AvailablePlants are exposed to a wide range of stresses and they respond to these stressed conditions by the activation of stress responsive genes. Microsatellites occur at thousands of locations in expressed regions, additionally, they have a higher mutation rate than other areas of DNA leading to genetic markers. A computational method was developed to capture exact repeat motifs in EST sequences of Solanum melongena and extract the sequences. This mathematical algorithm based repeat detection program searches for perfect repeats in the EST sequences without the need to specify the pre-defined repeat pattern for di, tri, tetra-, upto octa repeat with the exception of mono nucleotides. Hexa repeat motifs were abundantly available followed by tetra and di repeats. The identified EST derived markers with repeat motif can be used to identify the precise inheritance pattern of the expressed region. The redundant ESTs were filtered and 121 unique ESTs in S. melongena were found to have potential markers. These were spanned across 19 major proteins superfamilies having conserved domains associated with abiotic stress tolerance. These robust and potential EST derived markers are linked to genes that encode for specific traits providing tolerance against abiotic stresses which is useful in marker-assisted breeding programs.Not Availabl

Identification of dehydrin markers in Capsicum annuum for development of drought tolerant crops in changing environment

Not AvailableAim: Dehydrins contribute to drought stress tolerance in plants. Identification of dehydrin markers and incorporating in plant breeding programmes is essential for improving drought tolerance. This study focus on identifying markers linked to dehydrin protein for enhancing drought tolerance in Capsicum annuum. Methodology: The publically available 1,15,000 plus EST collection of C. annuum were explored for identifying the presence of markers. A computer program has been developed to read and analyse large fragments of nucleotide sequences and for locating the microsatellite repeat motifs in the EST collections of C. annuum. The program searches for exact motifs, a string of repeated units, with all possible combinations of motif occurrences. Results: Based on conserved protein domains, several ESTs were identified with potential markers. Among them, one-third was predominated by dehydrin marker also having high sequence similarity. Three dehydrin markers found to be highly conserved and robust and was associated at high frequency with the DHN protein. Interpretation: The potential dehydrin markers are directly linked to genes that encode trait specific characteristics and are much useful in marker-assisted breeding programs for imparting drought tolerance