40 research outputs found
A 1,000-loci transcript map of the barley genome: new anchoring points for integrative grass genomics
An integrated barley transcript map (consensus
map) comprising 1,032 expressed sequence tag
(EST)-based markers (total 1,055 loci: 607 RFLP, 190
SSR, and 258 SNP), and 200 anchor markers from previously
published data, has been generated by mapping
in three doubled haploid (DH) populations. Between
107 and 179 EST-based markers were allocated to the
seven individual barley linkage groups. The map covers
1118.3 cM with individual linkage groups ranging from
130 cM (chromosome 4H) to 199 cM (chromosome
3H), yielding an average marker interval distance of
0.9 cM. 475 EST-based markers showed a syntenic
organisation to known colinear linkage groups of the
rice genome, providing an extended insight into the
status of barley/rice genome colinearity as well as
ancient genome duplications predating the divergence
of rice and barley. The presented barley transcript map
is a valuable resource for targeted marker saturation
and identiWcation of candidate genes at agronomically
important loci. It provides new anchor points for
detailed studies in comparative grass genomics and will
support future attempts towards the integration of
genetic and physical mapping information