STRUCTURAL DETERMINATION OF L-ASPARAGINASE II OF STREPTOMYCES ALBIDOFLAVUS AND INTERACTION ANALYSIS WITH L-ASPARAGINE AND CEFOTAXIME

Objective: L-Asparaginase enzyme possesses a crucial role in the treatment of various hematologic malignancies. The current study focuses on homology modeling and interaction analysis of L-Asparaginase proteins belonging to Streptomyces albidoflavus (S. albidoflavus) with the essential ligand L-Asparagine and subsequent analysis with essential β-lactam antibiotic Cefotaxime. Methods: The process of understanding Asparaginase interactions primarily involved structure determination of WP_096097608, WP_095730301, which is achieved by GalaxyTBM, I-TASSER and SWISS-MODEL. Further, the S. albidoflavus Asparaginase proteins are subjected to GalaxySite and Autodock Vina of PyRx analysis. Results: The GalaxyTBM predicted structures of both the proteins are found promising on various validation studies. The two Asparaginase proteins exhibited high binding affinities of-6.8 and-6.5 kcal/mol with Cefotaxime and-5.1 and-4.9 kcal/mol towards Asparagine. The protein WP_096097608 residues forming hydrogen bonds with L-Asparagine are also analysed to involve in interaction with Cefotaxime on individual docking analysis. Conclusion: The current findings details the two S. albidoflavus Asparaginase proteins affinity towards L-Asparagine, hence can be assessed further for immunogenicity studies. In addition to the above findings, an attempt is made to find the L-Asparaginase binding possibilities with non-metals that identified an essential β-lactam antibiotic Cefotaxime to be an effective inhibitor. This study helps in understanding the interactions of L-Asparaginase with Cefotaxime, as intake of antibiotics between the phases of chemotherapy is observed to treat various infections and also as an antibiotic to microbes that utilize Asparaginase as a vital enzyme

Value Added Products Generation from Sugarcane Bagasse and Its Impact on Economizing Biorefinery and Sustainability of Sugarcane Industry

Augmenting value-added products generation with the biorefinery process of sugar cane by utilizing the by-products helps to achieve a more sustainable model of the sugarcane industry and in turn, contributes to the circular economy. Among the value-added products produced from sugarcane waste, functional foods offer additional health benefits besides their nutritional and calorific value. In recent years non-digestible sugars gained interest as potential prebiotic functional foods which benefit the host without increasing calorific value. These sugars are produced by the breakdown of carbohydrate polymers like cellulose and xylan, by thermochemical treatment or by enzymatic hydrolysis, or a combination of both. Sugar cane bagasse (SB) is an economical source of xylan which can serve as the substrate for xylooligosaccharides (XOS), xylobiose, xylitol, and ethanol. Cellulases, xylanases, and ligninases have wide applications in food processing, agro-fiber, pharmaceutical, and the paper and pulp industries including nutraceuticals production, where these enzymes provide eco-friendly alternatives to some chemical processes and help to reduce environmental impact. Conventional thermochemical methods for nutraceuticals production require chemicals that result in the release of toxic byproducts thus requiring additional steps for refining. In this context, the sustainable and eco-friendly processes for the production of nutraceuticals require employing biocatalysts like microbial enzymes or microbes as a whole, where in addition to averting the toxic byproducts the refining process requires lesser steps. The present chapter discusses the current research and challenges in the production of value-added products from sugarcane byproducts and their contribution to the sustainability of the sugarcane industry

DEVELOPMENT OF NEW VALIDATED METHOD FOR THE DETERMINATION OF SULTAMICILLIN TOSYLATE IN TABLET DOSAGE FORMS BY RP-HPLC

ABSTRACT This paper presents a RP-HPLC method for the estimation of Sultamicillin Tosylate in tablets. The process was carried out on C 18 column (5 μm, 150mm x 4.6 mm, i.d) using phosphate buffer (pH 4.0), acetonitrile in the ratio 60:40 respectively as a mobile phase at a flow rate of 1mL/min. Wavelength was fixed at 230 nm. The retention time of Sultamicillin Tosylate was found to be 9.022. The developed method is rapid, accurate, simple, reliable and sensitive method and it can be used for estimation of the drug in tablets

Genetic Variability and Divergence of Morphological and Seed Quality Traits of Greengram (Vigna radiata L.) Genotypes

Forty greengram genotypes were evaluated for their morphological traits and to find the extent of genetic variability. Analysis of variance revealed that the genotypes were highly significant for all the characters studied, indicating the existence of considerable magnitude of variability. High (>20%) phenotypic co-efficient of variation and high genotypic co-efficient of variation for seed yield (kg/4.05 m2) in the present investigation was noticed and indicating the minimal influence of environment and presence of high genetic variability for the trait in the experimental material. Hence, selection based on phenotype in these genotypes can also be effective for improvement of seed yield. High heritability to plant height (cm), days to 50% flowering, days to maturity, pod length, 100 seed weight, protein estimation and medium heritability to seed yield (kg/4.05 m2). High GAM to plant height and seed yield demonstrates the presence of additive gene effect indicating effectiveness of selection for improvement of these traits. Mahalanobis D2 analysis suggested the maximum contribution of seed yield (74.87%) towards genetic diversity followed by Plant height (8.08%), Days to Maturity (7.69%), Pod length (4.36%), Days to 50% flowering (3.59%), Seedling Dry Weight (0.64%), Protein Estimation (0.64%), 100 seed weight (0.13%). All 40 genotypes were grouped into 12 clusters. The clustering pattern revealed that genetic diversity was associated with geographical diversity in the present research. Based on mean performances, the genotypes PUSA-9072, MLGG-21-2, IC-436557, MLGG-21-6, RMP-21-11, Gouri, MLGG-21-3, MGG-512, MGG-519 from these clusters can be directly used as parents in the hybridization programme. The output of this study is characterization of greengram genotypes for DUS characters and other traits. This study helps in identification of genotypes with suitable traits, helps in registration of lines with PPV and FRA and the material can be used in breeding programmes