2 research outputs found
Elucidating the Influence of Tumor Presence on the Polymersome Circulation Time in Mice
The use of nanoparticles as tumor-targeting agents is steadily increasing, and the influence
of nanoparticle characteristics such as size and stealthiness have been established for a large number of
nanocarrier systems. However, not much is known about the impact of tumor presence on nanocarrier
circulation times. This paper reports on the influence of tumor presence on the in vivo circulation
time and biodistribution of polybutadiene-polyethylene oxide (PBd-PEO) polymersomes. For thi
Uptake and subcellular distribution of radiolabeled polymersomes for radiotherapy
Polymersomes have the potential to be applied in targeted alpha radionuclide therapy, while in addition preventing release of recoiling daughter isotopes. In this study, we investigated the cellular uptake, post uptake processing and intracellular localization of polymersomes. Methods: High-content microscopy was used to validate polymersome uptake kinetics. Confocal (live cell) microscopy was used to elucidate the uptake mechanism and DNA damage induction. Intracellular distribution of polymersomes in 3-D was determined using super-resolution microscopy. Results: We found that altering polymersome size and concentration affects the initial uptake and overall uptake capacity; uptake efficiency and eventual plateau levels varied between cell lines;