Selection of resistant upland cotton genotypes challenged with aggressive isolates of Meloidogyne incognita race 3

Received: July 11th, 2022 ; Accepted: August 19th, 2022 ; Published: August 25th, 2022 ; Correspondence: [email protected] study aimed to select populations of M. incognita race 3 for screening cotton genotypes as part of a breeding program for the development of resistant cotton cultivars. Five isolates of M. incognita race 3, collected in Western Paraná, Brazil, were tested for virulence and aggressiveness against the cotton cultivars FM966 (susceptible), IAC 24 (resistant), CD 409, and FMT 701 (moderately resistants) under greenhouse conditions, and following a factorial design with five replicates. Thirty-one cotton genotypes were screened against the three most aggressive isolates of M. incognita race 3 tested before and kept under greenhouse conditions following a factorial design with five replicates. Experiments run under greenhouse conditions had single cotton plants inoculated with 5,000 eggs/J2 of M. incognita and were assessed at 120 days after inoculation considering the variables gall index, egg mass index, total eggs, and reproduction factor. The same genotypes tested under greenhouse conditions were also grown in a field infested with M. incognita race 3 in a randomized block design with 10 replicates. In the field, the M. incognita population was monitored by the quantification of J2 forms in soil samples collected before sowing, 60 days after sowing (DAS), and 120 DAS. A gall index score was used to evaluate the roots of cotton genotypes at 120 DAS. The isolate from Umuarama was the most aggressive, followed by Moreira Sales and Iporã. The genotypes CD 05-419, CD 05-945, CD 05-1087, and CD 05-1170 showed resistance against M. incognita race 3 under greenhouse and field conditions

In vitro and in vivo ocular biocompatibility of electrospun poly(ɛ-caprolactone) nanofibers.

Biocompatibility is a requirement for the development of nanofibers for ophthalmic applications. In this study, nanofibers were elaborated using poly(ε-caprolactone) via electrospinning. The ocular biocompatibility of this material was investigated. MIO-M1 and ARPE-19 cell cultures were incubated with nanofibers and cellular responses were monitored by viability and morphology. The in vitro biocompatibility revealed that the nanofibers were not cytotoxic to the ocular cells. These cells exposed to the nanofibers proliferated and formed an organized monolayer. ARPE-19 and MIO-M1 cells were capable of expressing GFAP, respectively, demonstrating their functionality. Nanofibers were inserted into the vitreous cavity of the rat's eye for 10days and the in vivo biocompatibility was investigated using Optical Coherence Tomography (OCT), histology and measuring the expression of pro-inflammatory genes (IL-1β, TNF-α, VEGF and iNOS) (real-time PCR). The OCT and the histological analyzes exhibited the preserved architecture of the tissues of the eye. The biomaterial did not elicit an inflammatory reaction and pro-inflammatory cytokines were not expressed by the retinal cells, and the other posterior tissues of the eye. Results from the biocompatibility studies indicated that the nanofibers exhibited a high degree of cellular biocompatibility and short-term intraocular tolerance, indicating that they might be applied as drug carrier for ophthalmic use

Magnetic And Superconducting Properties Of Ru Sr2 Gd1.5 Ce0.5 Cu2 O10-δ Samples: Dependence On The Oxygen Content And Aging Effects

The magnetic and superconducting properties of Ru Sr2 Gd1.5 Ce0.5 Cu2 O10-δ polycrystalline samples with different oxygen-doping levels are presented. A strong suppression of the superconducting temperature (Tc), as well as a reduction in the superconducting fraction, occurs as the oxygen content is reduced by annealing the samples in oxygen-deprived atmospheres. Drastic changes in the electrical resistivity are observed above Tc, possibly associated with oxygen removal, mainly from grain boundaries. However, the magnetic ordering is relatively less affected by the changes in oxygen content of the samples. The spin-glass transition is enhanced and shifted to higher temperatures with the reduction in oxygen content. This could be correlated with an increase in the spin disorder and frustration for the oxygen-depleted samples. Also, the same oxygen-vacancy-induced disorder could explain the reduction in the fraction of the sample showing antiferromagnetic order. We also report significant changes in the measured properties of the samples as a function of time. © 2005 The American Physical Society.7113Felner, I., Asaf, U., Levi, Y., Millo, O., (1997) Phys. Rev. B, 55, p. 3374. , PRBMDO 0163-1829 10.1103/PhysRevB.55.R3374Bernhard, C., Tallon, J.L., Niedermayer, Ch., Blasius, Th., Golnik, A., Brücher, E., Kremer, R.K., Ansaldo, E.J., (1999) Phys. Rev. B, 59, p. 14099. , PRBMDO. 0163-1829. 10.1103/PhysRevB.59.14099Awana, V.P.S., Karppinen, M., Yamauchi, H., (2003) Studies of High Tc Superconductors, 46, p. 77. , edited by A. V. Narlikar (Nova Science Publishers, New YorkMatvejeff, M., Awana, V.P.S., Jang, L.-Y., Liu, R.S., Yamauchi, H., Karppinen, M., (2003) Physica C, 392-396, p. 87. , PHYCE6 0921-4534Cardoso, C.A., Araujo-Moreira, F.M., Awana, V.P.S., Kishan, H., Takayama-Muromachi, E., De Lima, O.F., (2004) Physica C, 405, p. 212. , PHYCE6 0921-4534Cardoso, C.A., Araujo-Moreira, F.M., Awana, V.P.S., Takayama-Muromachi, E., De Lima, O.F., Yamauchi, H., Karppinen, M., (2003) Phys. Rev. B, 67, p. 020407. , PRBMDO 0163-1829 10.1103/PhysRevB.67.020407Shi, L., Li, G., Fan, X.J., Feng, S.J., Li, X.-G., (2003) Physica C, 399, p. 69. , PHYCE6 0921-4534Felner, I., Asaf, U., Ritter, F., Klamut, P.W., Dabrowski, B., (2001) Physica C, 364-365, p. 368. , PHYCE6 0921-4534Felner, I., Asaf, U., Galstyan, E., (2002) Phys. Rev. B, 66, p. 024503. , PRBMDO 0163-1829 10.1103/PhysRevB.66.024503Felner, I., Asaf, U., Levi, Y., Millo, O., (2000) Physica C, 334, p. 141. , PHYCE6 0921-4534 10.1016/S0921-4534(00)00250-1Awana, V.P.S., Ansari, M.A., Gupta, A., Saxena, R.B., Kishan, H., Buddhikot, D., Malik, S.K., (2004) Phys. Rev. B, 70, p. 104520. , PRBMDO 0163-1829 10.1103/PhysRevB.70.104520Yoshizawa, H., Mitsuda, S., Aruga, H., Ito, A., (1987) Phys. Rev. Lett., 59, p. 2364. , PRLTAO 0031-9007 10.1103/PhysRevLett.59.2364Binder, K., Young, A.P., (1986) Rev. Mod. Phys., 58, p. 801. , RMPHAT 0034-6861 10.1103/RevModPhys.58.801Mydosh, J.A., (1993) Spin Glasses An Experimental Introduction, , Taylor & Francis, LondonFelner, I., Galstyan, E., Herber, R.H., Nowik, I., (2004) Phys. Rev. B, 70, p. 094504. , PRBMDO 0163-1829 10.1103/PhysRevB.70.094504Shengelaya, A., Khasanov, R., Eschenko, D.G., Felner, I., Asaf, U., Savić, I.M., Keller, H., Müller, K.A., (2004) Phys. Rev. B, 69, p. 024517. , PRBMDO. 0163-1829. 10.1103/PhysRevB.69.024517Xue, Y.Y., Cao, D.H., Lorenz, B., Chu, C.W., (2002) Phys. Rev. B, 65, p. 020511. , PRBMDO 0163-1829 10.1103/PhysRevB.65.02051

Influencia del manejo del cultivo de maíz sobre el microbioma: implicancias en la pudrición de raíz y tallo

PosterActualmente, una de las principales limitantes para el cultivo de maíz son las enfermedades causadas por hongos. Entre las enfermedades fúngicas reportadas en las últimas campañas se encuentra la pudrición de raíz y tallo (PRT), producida por un complejo de patógenos que habitan en los rastrojos y/o suelo. En los últimos años, esta enfermedad ha incrementado su frecuencia e intensidad, lo cual está asociado a siembras tardías, maíces de segunda y otoños húmedos. Además, el aumento en la densidad de siembra junto con la producción continua del cultivo e inclusión de gramíneas en la secuencia, favorecerían la enfermedad. En este sentido, la intensificación del uso del suelo, implica su progresivo deterioro, generando un desbalance en sus propiedades microbianas, químicas y físicas, lo cual podría explicar el aumento de la incidencia de la enfermedad. Se sabe que algunas prácticas de manejo, como el monocultivo, también tienen efectores estresores sobre el maíz. Por el contrario, la rotación de cultivos, promueve la producción y sanidad de los cultivos principalmente a través de una mejora en las condiciones del suelo (mejor estructura, incremento del contenido de nutrientes, aumento de la biomasa y actividades microbianas, interrupción de los ciclos de los patógenos).Instituto de Patología VegetalFil: Serri, Dannae Lilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Serri, Dannae Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Bernardi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: De Rossi, R.L. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentin

Angiotensin-converting enzyme inhibitor protects against cisplatin nephrotoxicity by modulating kinin B1 receptor expression and aminopeptidase P activity in mice

Cisplatin is a highly effective chemotherapeutic agent. However, its use is limited by nephrotoxicity. Enalapril is an angiotensin I-converting enzyme inhibitor used for the treatment of hypertension, mainly through the reduction of angiotensin II formation, but also through the increase of kinins half-life. Kinin B1 receptor is associated with inflammation and migration of immune cells into the injured tissue. We have previously shown that the deletion or blockage of kinin B1 and B2 receptors can attenuate cisplatin nephrotoxicity. In this study, we tested enalapril treatment as a tool to prevent cisplatin nephrotoxicity. Male C57Bl/6 mice were divided into 3 groups: control group; cisplatin (20 mg/kg i.p) group; and enalapril (1.5 mg;kg i.p) + cisplatin group. The animals were treated with a single dose of cisplatin and euthanized after 96 h. Enalapril was able to attenuate cisplatin-induced increase in creatinine and urea, and to reduce tubular injury and upregulation of apoptosis-related genes, as well as inflammatory cytokines in circulation and kidney. The upregulation of B1 receptor was blocked in enalapril + cisplatin group. Carboxypeptidase M expression, which generates B1 receptor agonists, is blunted by cisplatin + enalapril treatment. The activity of aminopeptidase P, a secondary key enzyme able to degrade kinins, is restored by enalapril treatment. These findings were confirmed in mouse renal epithelial tubular cells, in which enalaprilat (5 μM) was capable of decreasing tubular injury and inflammatory markers. We treated mouse renal epithelial tubular cells with cisplatin (100 μM), cisplatin+enalaprilat and cisplatin+enalaprilat+apstatin (10 μM). The results showed that cisplatin alone decreases cell viability, cisplatin plus enalaprilat is able to restore cell viability, and cisplatin plus enalaprilat and apstatin decreases cell viability. In the present study, we demonstrated that enalapril prevents cisplatin nephrotoxicity mainly by preventing the upregulation of B1 receptor and carboxypeptidase M and the increased concentrations of kinin peptides through aminopeptidase activity restoration

Spatial and seasonal variations in the stable carbon isotopic composition of methane in stream sediments of eastern Amazonia

The stable carbon isotopic composition of methane (δ13 C-CH4) gas bubbles formed in the sediments of three Amazonian streams was determined over a 5-yr period. The study sites were two ' 'várzea' floodplain (Açu and Maicá) and one 'terra-firme' (Jamaraquá) streams. The δ 13C of sedimentary organic matter (SOM) from the surrounding vegetation and bottom sediments were also determined. The mean δ13C value of SOM was lower in the terra-firme (-29.6‰) than in the várzea stream (-23.8‰) as a result of less C4 plant deposition in the former. The δ 13C-CH4 values varied systematically both seasonally and spatially among the sites during all 5 yr of the study, in association with changes during hydrologic cycle. Overall, the variation in values of δ 13C-CH4 during the high water phase covered a narrower range of values, -63 to -56‰. Contrastively, during the low water phase the δ 13C-CH4 values for várzea and terra-firme streams are different and are in direct opposition. At this phase, the δ13C-CH4 at terra-firme stream is at least 20‰ depleted of 13C compared to várzea streams. Changes in organic matter sources, water levels and associated microbial degradation processes control the observed seasonal and spatial variations in net stable carbon isotopic composition of methane

A Biased Review of Sociophysics

Various aspects of recent sociophysics research are shortly reviewed: Schelling model as an example for lack of interdisciplinary cooperation, opinion dynamics, combat, and citation statistics as an example for strong interdisciplinarity.Comment: 16 pages for J. Stat. Phys. including 2 figures and numerous reference

A feasibility cachaca type recognition using computer vision and pattern recognition

Brazilian rum (also known as cachaça) is the third most commonly consumed distilled alcoholic drink in the world, with approximately 2.5 billion liters produced each year. It is a traditional drink with refined features and a delicate aroma that is produced mainly in Brazil but consumed in many countries. It can be aged in various types of wood for 1-3 years, which adds aroma and a distinctive flavor with different characteristics that affect the price. A research challenge is to develop a cheap automatic recognition system that inspects the finished product for the wood type and the aging time of its production. Some classical methods use chemical analysis, but this approach requires relatively expensive laboratory equipment. By contrast, the system proposed in this paper captures image signals from samples and uses an intelligent classification technique to recognize the wood type and the aging time. The classification system uses an ensemble of classifiers obtained from different wavelet decompositions. Each classifier is obtained with different wavelet transform settings. We compared the proposed approach with classical methods based on chemical features. We analyzed 105 samples that had been aged for 3 years and we showed that the proposed solution could automatically recognize wood types and the aging time with an accuracy up to 100.00% and 85.71% respectively, and our method is also cheaper.info:eu-repo/semantics/publishedVersio