Determination of misonidazole and desmethylmisonidazole in plasma by high-performance liquid chromatography with reductive electrochemical detection

A reversed-phase high-performance liquid chromatographic method with reductive mode electrochemical detection was developed for the determination of misonidazole and desmethylmisonidazole in plasma. A thin-layer amperometric detector with glassy carbon working electrode was used to detect the nitroimidazoles at a potential of -0.60 V. The calibration curves were linear. The within-day and day-to-day coefficients of variation were below 3% for plasma misonidazole concentrations of 6-60 mg/1 and 1-15 mg/1 for desmethylmisonidazole. Electrochemical detection limits were between 2 and 4 pg, which is about 10-20 times lower than that obtained by detection at 323 nm. Limits of quantitation of the nitroimidazoles in plasma were in the order of 1-2 μg/l. Under the described conditions other nitroimidazoles and nitro compounds can also be detected with ultimate sensitivity by reductive mode electrochemical detection

Mono- and biphasic plasma concentration-time curves of mesalazine from a 500 mg suppository in healthy male volunteers controlled by the time of defecation before dosing

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Saturable active tubular reabsorption in the renal clearance of mesalazine in human volunteers

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(S)-UH301, a silent 5-HT(1A) receptor antagonist, enhances plasma cortiocosterone levels in the rat

The putative silent 5-HT(1A) receptor antagonist (S)-UH301 (S-5-fluoro-8-hydroxy-2-(di-n-propylamino)tetralin) dose-dependently enhanced (3 to 10 mg/kg, sc) plasma corticosterone levels in undisturbed rats, an effect shared with 5-HT(1A) receptor agonists. (S)-UH301 did not influence plasma glucose levels. This unexpected finding may be indicative of a partial 5-HT(1A)-receptor agonistic effect of (S)-UH301, or reveals a hitherto unknown mechanism of action in (S)-UH301

Liver and gut mucosa acetylation of mesalazine in healthy volunteers

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