Infl uence of indole-3-butyric acid and triazole compounds on the photosynthetic pigments and biochemical constituents of Withania somnifera (L.) Dunal

Withania somnifera (L.) Dunal is popularly known as ashwagandha and also called Indian Ginseng and Winter Cherry. It is an important medicinal plant used in Indian System of Medicines like Ayurveda, Siddha, and Unani. The root of this plant has nutritional and medicinal values out of which health foods and herbal tonic are prepared. The root has high demand in pharmaceutical and nutraceutical industries so that it is essential to increase the root yield. This is new phenomenon to increase root yield as well as improve the biochemical constituents by using plant growth regulators. In the present investigation, the influence of indole-3-butyric acid (IBA) and triazole compounds viz., triadimefon (TDM) and propiconazole (PCZ) on the photosynthetic pigments and biochemical constituents of ashwagandha. Plants were treated with IBA 2.5 mg/L, TDM 20 mg/L, and PCZ 20 mg/L separately by soil drenching on 50, 90, and 130 days after sowing (DAS). Plants were analyzed randomly on 60, 100, and 140 DAS and it’s parameters like, photosynthetic pigments (total chlorophyll, carotenoids, anthocyanin, and xanthophylls) in leaf and biochemical constituents (starch, protein and amino acids) in leaf, stem and root organs of ashwagandha. It was determined that total chlorophyll, carotenoids, anthocyanin, and xanthophylls, starch, protein and amino acids content were increased in all the treatments. Among the treatments, triazole compounds showed beneficial due to the enhanced the photosynthetic pigments and increased biochemical contents higher level than followed by IBA treatment. From our results, it can be concluded that the triazole shows great significance application at low concentration could be a potential agronomical tool for successfully cultivation of this medicinally important root crops. Triazole compounds enhanced the photo-assimilate to shifting partition from leaves to roots and also alter mineral uptake and plant nutrition, This characters’ can be employed to satisfy needs of enhanced the photosynthetic pigments and biochemical constituents in ashwagandha

Eff ect of triazole compounds on induced changes in growth biomass and biochemical content of white radish (Raphanus sativus L.)

Radish (Raphanus sativus L.), belongs to the family Brassicaceae is an important vegetable crop and cultivated all over the India. The tuber of radish is used to prepare the salad and for the preparation of various health dishes. It is rich in vitamin ‘C’ and minerals. It is also used as medicinal properties such as currying liver disorders, jaundice, and various physiological disorders. In the present investigation, the effect of triazole compounds such as triadimefon (TDM) and hexaconazole (HEX) on radish. The plants were given separate treatment with 1 L of water solution containing 20/mg TDM and 1 L of water solution containing 20/mg HEX on 23, 35, and 53 days after sowing (DAS) by soil drenching method. The plants were harvested randomly on 30, 45, and 60 DAS for the determination of growth of tuber, shoot, and biochemical contents viz., starch, protein, and amino acid both in the control and triazole treated plants. From the results it is observed that the triazole treated plants showed pronounced effect in increasing the tuber fresh and dry biomass, protein, amino acid, and starch content in both shoot and tuber tissues of radish, whereas the shoot fresh and dry biomass were decreased when compared to control plants of radish

BIOCHEMICAL CONSTITUENTS OF WITHANIA SOMNIFERA UNDER THE INDOLE-3-BUTYRIC ACID AND TRIAZOLE SOIL DRENCHING TECHNIQUES

The present study aimed at understanding the effect of indole-3-butyric acid (IBA) and triazole compounds viz., triadimefon (TDM) and propiconazole (PCZ) on the biochemicals of ashwagandha. Treatments were given on 50, 90 and 130 days after sowing (DAS). Biochemical constituents such as proline, glycine betaine and total alkaloids content were determined. It was observed that proline, glycine betaine and alkaloids content were enhanced by TDM and PCZ than followed by IBA treatment when compared to control for respective growth stages.  Among the treatments, triazole compounds caused pronounced effect to the biochemical accumulation in higher level when compared to IBA treatment. These results suggest that, triazole active compounds act as a growth regulator also influence hormonal balance and great significance, which is helpful to satisfy the needs of enhance the biochemical contents in Ashwagandha