Detection of Circulating Filarial Antigen

Affinity binding to specific solid phase antibody, immune complex binding to Raji cells or PEG precipitation of immune complexes was used in conjunction with Western blotting to detect circulating filarial antigen in filarial sera. A high molecular weight antigen was present in free as well as complex form. PEG precipitation method revealed specific filarial antigens in the low molecular weight region also

Increased serum levels of anti-filarial (IgA) antibodies in patients with tropical pulmonary eosinophilia

Tropical Pulmonary Eosinophilia (TPE) is diagnosed on the basis of high peripheral eosinophilia associated with clinical symptoms and signs. Elevated levels of total and antifilarial immunoglobulins is one of the characteristic features of TPE. Ten clinically diagnosed TPE patients and ten controls were compared for their anti-filarial and anti-ascaris antibody levels of classes IgG, IgM and IgA. While, IgG antibodies exhibited considerable cross reactivity between Ascaris and Filarial antigens, IgM antibodies showed nonspecific binding to filarial antigens. However, IgA antibodies were found to discriminate between TPE and control sera better than IgG and IgM antibodies

The Spatial Averaging Limit of Covariant Macroscopic Gravity - Scalar Corrections to the Cosmological Equations

It is known that any explicit averaging scheme of the type essential for describing the large scale behaviour of the Universe, must necessarily yield corrections to the Einstein equations applied in the Cosmological setting. The question of whether or not the resulting corrections to the Einstein equations are significant, is still a subject of debate, partly due to possible ambiguities in the averaging schemes available. In particular, it has been argued in the literature that the effects of averaging could be gauge artifacts. We apply the formalism of Zalaletdinov's Macroscopic Gravity (MG) which is a fully covariant and nonperturbative averaging scheme, in an attempt to construct gauge independent corrections to the standard Friedmann-Lemaitre-Robertson-Walker (FLRW) equations. We find that whereas one cannot escape the problem of dependence on \emph{one} gauge choice -- which is inherent in the assumption of large scale homogeneity and isotropy -- it is however possible to construct \emph{spacetime scalar} corrections to the standard FLRW equations. This partially addresses the criticism concerning the corrections being gauge artifacts. For a particular initial choice of gauge which simplifies the formalism, we explicitly construct these scalars in terms of the underlying inhomogeneous geometry, and incidentally demonstrate that the formal structure of the corrections with this gauge choice is identical to that of analogous corrections derived by Buchert in the context of spatial averaging of scalars.Comment: 18 pages, no figures, revtex4; v2 - minor clarifications added; v3 - minor changes in presentation to improve clarity, reference added, to appear in Phys. Rev.

Fate of the false monopoles: induced vacuum decay

We study a gauge theory model where there is an intermediate symmetry breaking to a meta- stable vacuum that breaks a simple gauge group to a U (1) factor. Such models admit the existence of meta-stable magnetic monopoles, which we dub false monopoles. We prove the existence of these monopoles in the thin wall approximation. We determine the instantons for the collective coordinate that corresponds to the radius of the monopole wall and we calculate the semi-classical tunneling rate for the decay of these monopoles. The monopole decay consequently triggers the decay of the false vacuum. As the monopole mass is increased, we find an enhanced rate of decay of the false vacuum relative to the celebrated homogeneous tunneling rate due to Coleman [1].Comment: 10 pages, 4 figure

Production & characterization of monoclonal antibodies to Mycobacterium tuberculosis

Background & objectives: Monoclonal antibodies (MAbs) against Mycobacterium tuberculosis H37Rv culture filtrate (CF) were raised by immunizing BALB/c mice and characterization was done. Attempts have been directed towards identifying mycobacterial antigens in biological fluids by employing polyclonal and monoclonal antibodies specific for M. tuberculosis. Immunohistologic studies, using MAbs for the localization of whole or fragmented bacilli in the biopsy specimens were also carried out. Methods: Intrasplenic IS and intraperitoneal IP routes of immunization, were compared. The MAbs were characterized for their isotype, binding specificity, nature of binding epitope, reactivity in immunoassays etc. Results: IS and IF’ routes of immunization, were compared and IP was found superior. Ten MAbs designated TRC l-10 were produced. Of these, 7 MAbs, TRC 1-7 reacted with the 30/31 kDa doublet (antigen 85 complex), TRC 8 with 12 kDa in addition to 30/31 kDa and TRC 9 and 10 with the 24 and 12 kDa antigens respectively. Six MAbs were classified as broadly cross reactive and 2 showed limited cross reactivity. TRC 8 and 10 showed species specificity. Employing TRC 8 in sandwich ELISA, antigen was detected in sera from 17 of 25 pulmonary tuberculosis patients and 3 of 20 controls. TRC 8 was found to be useful in detecting antigens specifically in M. tuberculosis and M. leprae infected tissues, by immunoperoxidase staining. Interpretation & conclusion: TRC 8 was found to be restricted in its reactivity to M. tuberculosis complex and M, leprae. TRC 8 may prove useful in immuno-diagnosis of tuberculosis

Anti-filarial IgG antibodies in patients with Bancroftian filariasis and tropical pulmonary eosinophilia

Anti-filarial (anti- B. malayi adult as well as anti- B. malayi microfilarial) IgG antibody levels were measured by enzyme linked immunosorbent assay (ELISA) in asymptomatic microfilaria carriers, acute, chronic and tropical pulmonary eosinophilia (TPE) patients, endemic and non-endemic controls. Controls from endemic areas had higher antibody titres compared with controls from non-endemic areas. The antibody response in different groups of filariasis patients was not stage specific. There were no association between clinical disease and antibody levels except in TPE. Though TPE patients had very high antibody levels, a proportion of them had low levels suggesting heterogeneity in TPE population

Modeling and simulation for the spread of Covid-19 in an Indian city: a case study

We present a case study on modeling and predicting the course of Covid-19 in the Indian city of Pune. The results presented in this paper are concerned primarily with the wave of infections triggered by the Delta variant during the period between February and June 2021. Our work demonstrates the necessity for bringing together compartmental stock-and-flow and agent-based models and the limitations of each approach when used individually. Some of the work presented here was carried out in the process of advising the local city administration and reflects the challenges associated with employing these models in a real-world environment with its uncertainties and time pressures. Our experience, described in the paper, also highlights the risks associated with forecasting the course of an epidemic with evolving variants

Backreaction of Cosmological Perturbations in Covariant Macroscopic Gravity

The problem of corrections to Einstein's equations arising from averaging of inhomogeneities ("backreaction") in the cosmological context, has gained considerable attention recently. We present results of analysing cosmological perturbation theory in the framework of Zalaletdinov's fully covariant Macroscopic Gravity. We show that this framework can be adapted to the setting of cosmological perturbations in a manner which is free from gauge related ambiguities. We derive expressions for the backreaction which can be readily applied in \emph{any} situation (not necessarily restricted to the linear perturbations considered here) where the \emph{metric} can be brought to the perturbed FLRW form. In particular these expressions can be employed in toy models studying nonlinear structure formation, and possibly also in N-body simulations. Additionally, we present results of example calculations which show that the backreaction remains negligible well into the matter dominated era.Comment: 17 pages, 5 figures, revtex4; v2 -- typos fixed, 1 reference updated, to appear in Phys Rev

Identification of circulating parasite antigen in patients with bancroftian filariasis

Because many cases of lymphatic filariasis cannot be diagnosed either clinically or by immunodiagnostic test based on antibody detection, recent efforts have been more directed towards developing methods for detecting parasite antigen in the blood or urine. Using a solid phase (Sepharose 4B) two-site immunoradiometric assay (IRMA) employing hyperimmune rabbit antifilarial antisera, we have previously shown (Hamilton et al., 1984) that essentially all cases ofpatent (ie. microfilaremic) infection in patients with bancroftian filariasis can be detected by this semi-quantitative assay as well as some individuals with amicrofilaremic (i.e., 'cryptic') infection. The present communication reports the results of studies that identify a prominent circulating antigen detected by this IRMA in sera from patients with microfilaremia. The antigen was eluted from Sepharosebound rabbit polyclonal antiserum that had been reacted with known antigen positive sera. It was run in SDS-PAGE, blotted to nitrocellulose paper and identified autoradiographically using '25l-labelled rabbit antifilarial antiserum. Its high molecular weight (- 200 kD), stability to acid and boiling, and sensitivity to pronase and periodate suggest its being a glycoprotein. Isolation of this antigen will permit the development of specific reagents (such as monoclonal antibodies) which should enhance both the sensitivity and utility of the currently available antigen detection systems

Excursion Sets and Non-Gaussian Void Statistics

Primordial non-Gaussianity (NG) affects the large scale structure (LSS) of the universe by leaving an imprint on the distribution of matter at late times. Much attention has been focused on using the distribution of collapsed objects (i.e. dark matter halos and the galaxies and galaxy clusters that reside in them) to probe primordial NG. An equally interesting and complementary probe however is the abundance of extended underdense regions or voids in the LSS. The calculation of the abundance of voids using the excursion set formalism in the presence of primordial NG is subject to the same technical issues as the one for halos, which were discussed e.g. in arXiv:1005.1203. However, unlike the excursion set problem for halos which involved random walks in the presence of one barrier $\delta_c$, the void excursion set problem involves two barriers $\delta_v$ and $\delta_c$. This leads to a new complication introduced by what is called the "void-in-cloud" effect discussed in the literature, which is unique to the case of voids. We explore a path integral approach which allows us to carefully account for all these issues, leading to a rigorous derivation of the effects of primordial NG on void abundances. The void-in-cloud issue in particular makes the calculation conceptually rather different from the one for halos. However, we show that its final effect can be described by a simple yet accurate approximation. Our final void abundance function is valid on larger scales than the expressions of other authors, while being broadly in agreement with those expressions on smaller scales.Comment: 28 pages (18+appendices), 7 figures; v2 -- minor changes in sec 3.2, version published in PR