Production and Immobilization of Alpha Amylase by Using Bacillus subtilis

The α-amylase producing organisms were isolated from soil sample, such as Bacillus sp (2 isolates), Bacillus cereus (1 isolates), Bacillus subtilis (2 isolates) by basal medium and identified by standard biochemical test. The high yielding strain was identified as Bacillus subtilis and used for amylase production. Different  parameters  like temperature (10°C, room temperature (25°-30°C), 37°C & 55°C different pH (6.0, 6.5, 7.0, 7.5, 8.0) and incubation periods (1-5 days) were used for the α-amylase production. The isolate was inoculated to the production medium and incubated, then it was centrifuged  and the supernatant containing crude enzyme extract is used for protein estimation and activity. It was found that α-amylase production and activity was high at 37°Cat pH 7.0 in 48 hours. The organism was grown in the optimum conditions mentioned above and the enzyme α-amylase was immobilized by alginate gel entrapment method. From the organism the plasmid and the chromosomal DNA was isolated and detected

Antibacterial Activity and Phytochemical Standardization of Rhinacanthus nasutus (White Crane)

            Plants are the most exclusive source of drugs for the majority of the words population and plant products constitute about 25% at prescribed medicines phytochemical tests have been performed in about 5,000 and nearly 1,100 species are exclusively exploted in 80% Ayurvedic, 46% Unani and 33% Allopathi medicine. The values of the present data indicated that ethanolic extract (5.8%) and aqueous extract (6.8%) showed higher extractive values when compared to other solvents. Ethyl acetate showed the least extraction value (0.9%). At the maximum concentration tested, (250µg) the organisms showed maximum sensitivity and the leaf extract proved to be better than the antibiotic disc Chloramphenicol (30mcg) which recorded 24mm as a zone of inhibition.  The promising alkaloid, Rhinacanthin has potent medicinal applications. It exhibits anti proliferate activity, antimicrobial activity against dermatophytes and inhibits platdent aggregation. In spite of it’s potential. It remains unexplored and under utilized and hence chosen for the present study

Studies on the effect of mercury on germination and biochemical changes of ground nut [Arachis hypogaea (L). var. VRI- 1] seedlings

The uptake and accumulation of mercury in various parts of the plants namely stem, root, leaf and seeds showed a gradual decrease with the steady increase in mercury treatment. It can be concluded that the VRI-1 variety of groundnut was proved to be tolerant to mercury. Hence it can it is recommended that the variety VRI – 1 can be cultivated in the soils contaminated with mercury and chloralkali  plants which use mercury as an electrode in cells for the manufacture of caustic soda and chlorine effluent. This will prevent considerably the extent of damage caused by mercuryon ground nut to a certain extent

ETHNOBOTANICAL SURVEY OF CHITTERI HILLS OF EASTERN GHATS IN DHARMAPURI DISTRICT-TAMILNADU

Flora and fauna diversities are two facts components of biodiversity which covers the variety and variability of species. A survey was conducted to study the rare plants of chitteri hills, Tamil Nadu, India. 96 Plants Belonging to 48 Family, 90 Genus and 96 species were Documented Plants totally were under thorough investigation. Of these monocots are represented by 7 species belonging to 7 genera and 4 families, while dicot contributed by 87 species belonging to 82 genera and 39 families, Ferns 2 families and 2 genera, Fungi 1 family and 1 Genera. Plant species were recorded and identified during the survey. The results show that the habitat of plants belongs to herbs, shrubs and tree species. But, we have observed only minimum tree species. Of these monocots are represented by 7 species belonging to 7 genera and 4 families, while dicots contributed by 87 species belonging to 82 genera and 39 families, Ferns 2 families and 2 genera, Fungi 1 family and 1 Genera. The study decipts that Chitteri Hills have different variety of plants distributed all over the mountain