2,581 research outputs found
Automated System for Detection of Apple Purity and Its Grading
It is always a common problem for all the people to identify the purity of all the fruits that has been purchased from the �fruit mandi� or local fruit stores. In this paper we would like to propose an idea for identifying the purity of apples. At present, among all the apples that are being sold in a shop, only few samples are collected and tested for purity in the laboratories by food Corporation of INDIA (FOI), which might not ensure that all the apples being sold in that respective shop/market are pure. In this paper we are proposing a device which can sense if the chosen apple is pure or not and which can be used by all the common people who are purchasing fruits from the market. Through this infections and disorders caused by fruits consumption can be eradicated to some extent
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The cardiomyocyte "redox rheostat": Redox signalling via the AMPK-mTOR axis and regulation of gene and protein expression balancing survival and death.
Reactive oxygen species (ROS) play a key role in development of heart failure but, at a cellular level, their effects range from cytoprotection to induction of cell death. Understanding how this is regulated is crucial to develop novel strategies to ameliorate only the detrimental effects. Here, we revisited the fundamental hypothesis that the level of ROS per se is a key factor in the cellular response by applying different concentrations of H2O2 to cardiomyocytes. High concentrations rapidly reduced intracellular ATP and inhibited protein synthesis. This was associated with activation of AMPK which phosphorylated and inhibited Raptor, a crucial component of mTOR complex-1 that regulates protein synthesis. Inhibition of protein synthesis by high concentrations of H2O2 prevents synthesis of immediate early gene products required for downstream gene expression, and such mRNAs (many encoding proteins required to deal with oxidant stress) were only induced by lower concentrations. Lower concentrations of H2O2 promoted mTOR phosphorylation, associated with differential recruitment of some mRNAs to the polysomes for translation. Some of the upregulated genes induced by low H2O2 levels are cytoprotective. We identified p21Cip1/WAF1 as one such protein, and preventing its upregulation enhanced the rate of cardiomyocyte apoptosis. The data support the concept of a "redox rheostat" in which different degrees of ROS influence cell energetics and intracellular signalling pathways to regulate mRNA and protein expression. This sliding scale determines cell fate, modulating survival vs death
Comparison of different methods of temporomandibular joint disc reconstruction - An animal model
The document attached has been archived with permission from the Australian Dental Association. An external link to the publisher’s copy is included. Article first published online: 12 MAR 2008The optimum method of temporomandibular joint (TMJ) reconstruction has not been defined despite numerous surgical treatments and several well controlled clinical trials. Animal models offer an experimental method allowing direct comparison of standardized surgical techniques. Advanced osteoarthrosis was induced bilaterally in 12 mature merino sheep. Three months later unilateral surgical reconstruction was performed. Four sheep had discectomy alone, four discectomy with fresh TMJ disc grafts, and four discectomy with fresh auricular grafts. All three surgical methods resulted in some reversal and repair of the osteoarthritic process, with the best result being from the auricular graft, next discectomy alone, next the disc graft, with the untreated osteoarthritic joint showing the most advanced pathosis. This study supports the role of surgical reconstruction in advanced degenerative disease of the temporomandibular joint, in particular, auricular graft reconstruction.Nobumi Ogi, Jun-Ichi Ishimaru, Kenichi Kurita, Yujiro Handa, Robert H.B. Jones, Alastair N. Gos
Genetic Variability in Candidate Plus Trees of \u3cem\u3eAcacia nilotica\u3c/em\u3e--An Important Fodder Tree under Agroforestry System
The realization of the effects of climate change redirected the focus on agroforestry, which deals with the management of land use system for increased sustainability. Enhancement of fodder production per unit area per unit time is essential to meet the demand from livestock sector for which different land use systems are being developed. Acacia nilotica is one of the important agroforestry species which fits into in silvipastoral system under agroforestry, in arid and semi-arid regions. It is known as babool, kikkar or Indian gum arabic, which has ability to improve nitrogen content of soil through its interaction with symbiotic bacteria on drought prone and degraded soils, tolerant to waterlogged conditions. It is an important source of fodder, the leaves and pods together will supplement the protein component in the livestock feed and when everything is dry; Acacias serve as the only green fodder source during peak summer season. It is a good source of timber, fuel wood, industrial source for the raw material tannin and as protective hedge and hence plays an important role in rural farming. The studies on maximization of forage production and wasteland development has reported that babool has highest survivability among fodder trees and has higher dry fodder production/tree/year. Tree growth and canopy diameter contributes to the production of green as well as dry fodder biomass. Assessing the variation within and among the provenance selections for different traits will help selection of genotypes for different use in the silvipastoral agroforestry system. In view of this, the present study was undertaken to study the genetic variability present in the candidate plus trees of different provenances collected from central India
NMA CO (J=1-0) Observations of the Halpha/Radio Lobe Galaxy NGC 3079: Gas Dynamics in a Weak Bar Potential and Central Massive Core
within 24 lines with 80 characters) We present ^12CO (1-0) observations in
the central 4.5 kpc (1 arcmin) of the Halpha/Radio lobe galaxy NGC 3079 with
the Nobeyama Millimeter Array. The molecular gas shows four components: a main
disk, spiral arms, a nuclear disk, and a nuclear core. The main disk extends
beyond our spatial coverage. The spiral arms are superimposed on the main disk.
The nuclear disk with about 600 pc radius has an intense concentration of
molecular gas. Its appearance on PV diagrams is indicative of oval motions of
the gas, rather than circular. The nuclear core is more compact than our
resolution. Though it is unresolved, the nuclear core shows a very high
velocity about 200 km/s even at the radius of about 100 pc on the PV diagram.
We propose a model that NGC 3079 contains a weak bar. This model explains the
observed features: the main disk and spiral arms result from gaseous x1-orbits
and associated crowding respectively. The nuclear disk arises from gaseous
x2-orbits. From the appearance of the spiral arms on the PV diagram, the
pattern speed of the bar is estimated to be 55+-10 km/s/kpc. The high velocity
of the nuclear core cannot be explained by our model for a bar. Thus we
attribute it to a central massive core with a dynamical mass of 10^9 Msun
within the central 100 pc. This mass is three orders of magnitude more massive
than that of a central black hole in this galaxy.Comment: 43 pages, 17 figures; ApJ, 573, 105, 200
Domain closure and action of uracil DNA glycosylase (UDG): structures of new crystal forms containing the Escherichia coli enzyme and a comparative study of the known structures involving UDG
The structures of a new crystal form of free Escherichia coli uracil DNA glycosylase (UDG), containing four molecules in the asymmetric unit, and two forms of its complex with the proteinaceous inhibitor Ugi, containing two and four crystallographically independent complexes, have been determined. A comparison of these structures and the already known crystal structures containing UDG shows that the enzyme can be considered to be made up of two independently moving structural entities or domains. A detailed study of free and DNA-bound human enzyme strengthens this conclusion. The domains close upon binding to uracil-containing DNA, whereas they do not appear to do so upon binding to Ugi. The comparative study also shows that the mobility of the molecule involves the rigid-body movement of the domains superposed on flexibility within domains
Genetic Evaluation of Leucaena Genotypes in Bundelkhand Region of Central India
During the 1970s and 1980s, Leucaena was known as the “miracle tree” native to Central America and Mexico, because of its worldwide success as a long-lived and highly nutritious forage tree. It is estimated to cover 2-5 million ha area worldwide. The genus Leucaena is one of the most widely grown tropical fodder trees and is the subject of extensive research. This is mainly due to its long life span; high productivity even under regular defoliation; its adaptation to wide climatic and edaphic tolerances; excellent palatability and digestibility and many uses including wood for timber and fuel wood. The species possesses enormous wealth of variability and great potential for economic yield which attract the breeders in utilizing the species in hybridization. A logical way to start any breeding programme is to survey the variations present in the germplasm. Precise information on the nature and degree of genetic divergence in respect of important traits is a prerequisite for undertaking meaningful breeding programme towards the improvement and conservation of a species.
Further an investigation into the nature and degree of divergence among populations will be useful in understanding the course of evolution and for classifying the tree population into groups based on the diversity, particularly when they are overlapping in one more characters. Therefore, the present study was conducted to estimate genetic divergence among different germplasm of genus Leucaena
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