Temperatura de flores e síliquas de canola (Brassica napus L.) durante ocorrência de geada.

Orientador: Genei Antonio Dalmago

Implications of Inflammatory Bowel Disease for reconstructive surgery in non-malignant urinary tract dysfunction:an International Continence Society working group report

Potential consequences of inflammatory bowel disease (IBD) need evaluation for patients considering urinary tract reconstruction for benign disease. A working group was formed by the International Continence Society, which considered urinary tract reconstruction in IBD.Methods Nominal group technique was used to derive consensus. Principal aspects of IBD assessment and surgery decision-making were agreed. A questionnaire was used to facilitate the generation of statements by a core focus group of experts, which were modified and ratified by the wider working group. This was followed by final voting by the full working group.Results General considerations included identification importance of the specialist IBD multi-disciplinary team. Peri-operative considerations recommended avoiding pre-operative fasting from midnight and using an enhanced recovery after surgery (ERAS) protocol. Selection of bowel segment, pre-operative optimisation and post-operative issues were considered for both Ulcerative colitis (UC) and Crohn’s disease. UC is not an absolute contraindication to urinary tract reconstruction using small or large bowel. Elective reconstructive surgery should wait at least three months following resolution of any acute UC flare-up to correct all abnormalities. Crohn’s disease is a high-risk disease for urinary tract reconstruction, even if in remission. In Crohn’s, reconstructive surgical options are limited by the location and extent of gastrointestinal segment(s) affected and the phenotype of disease.Conclusion The consensus opinion indicates that urinary tract reconstruction using bowel segments is feasible in carefully selected and optimised patients with IBD lacking alternative management options, provided there is access to appropriate multidisciplinary skills. UC is relatively low risk for surgical procedures, whereas Crohn’s has considerably increased risk of morbidity. The potential risks must be properly discussed with patients considering reconstructive urological procedures. Outcomes should be carefully monitored and published to identify the safety and efficacy of reconstructive surgery in IBD, including full description of the disease status

Soil-surface straw influences micrometeorological conditions affecting canola mortality during nighttime frosts.

Our objective was to measure alterations in the micrometeorological conditions surrounding canola seedlingsduring frost periods, and to quantify seedling mortality as a function of straw distribution on the ground surface. The data was acquired from 15 frosts in 2014. We used four treatments, comprising ground surface without straw (SWS), ground surface entirely straw-covered (SEC), sowing line without straw (SLW), and soil with preexisting surface straw (SES), over three experiments. Net radiation (NR), soil heat flux (G), air (Ta), leaf (Lf), rosette (Tr), and surface temperature (Ts), and plant mortality were evaluated. NR was higher in the SEC treatment and lower in the SLW treatment, whereas G was higher on straw-covered ground; Ts and Ta were lower in the SEC than in the other treatments during the most intense frosts. On 06/19, Tr in the SEC and SLW treatments was -0.66 °C and 0.42 °C, respectively; on 08/14, Lf was -3.62 °C and -2.88 °C in the SEC and SLW treatments, respectively. Plant mortality due to the frost on 06/19 was 30% in the SEC treatment, but 0% in the SLW treatment; the frost of 08/14 caused 33.8% mortality in the SEC treatment and 1.25% in the SLW treatment. This therefore showed that removing straw from the sowing line improved the microclimate around the plants, thus reducing canola mortality at the beginning of the growth cycle, which is when frost events most frequently occur. Keywords: air temperature, Brassica napus L., freezing, leaf temperature, microclimat

Structural characteristics and antiviral activity of multiple peptides derived from MDV glycoproteins B and H

<p>Abstract</p> <p>Background</p> <p>Marek's disease virus (MDV), which is widely considered to be a natural model of virus-induced lymphoma, has the potential to cause tremendous losses in the poultry industry. To investigate the structural basis of MDV membrane fusion and to identify new viral targets for inhibition, we examined the domains of the MDV glycoproteins gH and gB.</p> <p>Results</p> <p>Four peptides derived from the MDV glycoprotein gH (gHH1, gHH2, gHH3, and gHH5) and one peptide derived from gB (gBH1) could efficiently inhibit plaque formation in primary chicken embryo fibroblast cells (CEFs) with 50% inhibitory concentrations (IC₅₀) of below 12 μM. These peptides were also significantly able to reduce lesion formation on chorioallantoic membranes (CAMs) of infected chicken embryos at a concentration of 0.5 mM in 60 μl of solution. The HR2 peptide from Newcastle disease virus (NDVHR2) exerted effects on MDV specifically at the stage of virus entry (i.e., in a cell pre-treatment assay and an embryo co-treatment assay), suggesting cross-inhibitory effects of NDV HR2 on MDV infection. None of the peptides exhibited cytotoxic effects at the concentrations tested. Structural characteristics of the five peptides were examined further.</p> <p>Conclusions</p> <p>The five MDV-derived peptides demonstrated potent antiviral activity, not only in plaque formation assays in vitro, but also in lesion formation assays in vivo. The present study examining the antiviral activity of these MDV peptides, which are useful as small-molecule antiviral inhibitors, provides information about the MDV entry mechanism.</p