On the compliance of women engineers with a gendered scientific system

There has been considerable effort in the last decade to increase the participation of women in engineering through various policies. However, there has been little empirical research on gender disparities in engineering which help underpin the effective preparation, co-ordination, and implementation of the science and technology (S&T) policies. This article aims to present a comprehensive gendered analysis of engineering publications across different specialties and provide a cross-gender analysis of research output and scientific impact of engineering researchers in academic, governmental, and industrial sectors. For this purpose, 679,338 engineering articles published from 2008 to 2013 are extracted from the Web of Science database and 974,837 authorships are analyzed. The structures of co-authorship collaboration networks in different engineering disciplines are examined, highlighting the role of female scientists in the diffusion of knowledge. The findings reveal that men dominate 80% of all the scientific production in engineering. Women engineers publish their papers in journals with higher Impact Factors than their male peers, but their work receives lower recognition (fewer citations) from the scientific community. Engineers —regardless of their gender—contribute to the reproduction of the male-dominated scientific structures through forming and repeating their collaborations predominantly with men. The results of this study call for integration of data driven gender-related policies in existing S&T discourse

Ocular infection of mice with an avirulent recombinant HSV-1 expressing IL-4 and an attenuated HSV-1 strain generates virulent recombinants in vivo

PurposeTo assess the relative impact of overexpression of interleukin 2 (IL-2), interleukin 4 (IL-4), and interferon gamma (IFN-γ) expressing recombinant herpes simplex virus type 1 (HSV-1) on altering immune responses in ocularly infected mice.MethodsBALB/c mice were co-infected ocularly with avirulent HSV-1 strain KOS and avirulent recombinant HSV-1 expressing murine IL-4 (HSV-IL-4). Controls mice were co-infected with KOS + HSV-IL-2 or KOS + HSV-IFNγ. Following ocular infection, virus replication in the eye, corneal scarring (CS), and survival were determined. We also isolated recombinant viruses from eye and trigeminal ganglia of KOS + HSV-IL-4 infected mice.ResultsIn this study we found that ocular infection of BALB/c mice with a mixture of HSV-IL-4 and KOS resulted in increased death and increased eye disease. In contrast, when mice were infected in one eye with KOS and the other eye with HSV-IL-4 no death or eye disease was seen. Intraperitoneal co-infection of mice with KOS and HSV-IL-4 also did not result in HSV-1 induced death. Interestingly, ocular infection of mice with a mixture of HSV-IL-2 and KOS did not have any effect on severity of the disease in infected mice. We isolated recombinant viruses from KOS + HSV-IL-4 infected mice eye and trigeminal ganglia. Some of the isolated viruses were more neurovirulent then either parental virus. Infection of macrophages with IL-4 expressing virus down-regulated IL-12 production by macrophages.ConclusionsThese results suggest a role for IL-4 in suppression of immune response and generation of virulent viruses in vivo

The effect of different levels of Mentha piperita on some of the hematological, biochemical and immune parameters of Oncorhynchus mykiss

Due to the increased bacterial resistance to common antibiotics, there is a tendency towards using herbal extracts in order to increase the non-specific immune system. This study was conducted to evaluate the effects of different levels of Mentha piperita extract on hematological, biochemical and immune parameters of Oncorhynchus mykiss. For this purpose, fish with mean (±SD) weight of 32.2± 0.12 g were raised for 8 weeks in Vniro tanks (1800 l water), 80 fish to each tank and feeding with different levels of M. piperita (with concentrations of % 0, % 1, % 2.0 and % 3.0, three replicates were used for each concentration). At the end of the trial, blood samples were collected to determine some hematological, biochemical and immunity parameters in different groups and compared to one another. Results showed significant differences in RBC and WBC count, neutrophil percentage, haemoglobin (Hb), haematocrit (HCT) value, total protein (TP), IgM and lysozyme activity in fish fed M. piperita (especially with 2 and 3.0% concentrations) when compared with control group. The results suggest that M. piperita extract may enhance the non-specific immune system of O. mykiss. Thus, using this supplement especially at of 3.0% level as immunostimulants were recommended in O. mykiss diet

In vitro inhibition of growth in Saprolegnia sp. isolated from the eggs of Persian sturgeon Acipenser persicus (Pisces: Acipenseriformes) by Pseudomonas aeroginosa (PTCC:1430)

Saprolegnia is one of the most important agents decreasing the eggs survival rate in sturgeon hatcheries.There are some chemical substances for controlling the fungal infection of eggs. In this study, an attempt was made to introduce a germ negative bacterium, Pseudomonas aeroginosa (PTCC1430)(Persian Type Culture Collection) as a biocontrolling agent of water mold. Saprolegnia was isolated from the eggs of some infected Persian sturgeon, Acipenser persicus in a sturgeon hatchery and then was purified. P.aeroginosa was cultured in Potato dextrose Agar (PDB) media and then was prepared in 5 concentrations (103,104,105,106and107cfu.ml-1) while challenging with fungi in petri dishes under laboratory conditions.The results showed that by increasing the concentration of the bacteria in plates, hyphal growth of the fungi was reduced. The highest concentration of P. aeroginosa concentration (107) roughly stopped the -fungi growth and the Minimum Inhibitory Concentration (MIC) was 104cfu.m-l. Results in this study implied the potential of P. aeroginosa (PTCC1430) as a biological agent in controlling saprolegniosis

INVESTIGATION OF THE RELATIONSHIP BETWEEN PHASE ANGLE AND MICRO-ALBUMINURIA IN TYPE 2 DIABETIC PATIENTS WITH A HISTORY OF MORE THAN 5 YEARS OF THE DISEASE IN ILAM PROVINCE, IRAN

Introduction: Phase angle is the ratio between cell mass and fat-free tissue and a cell health indicator which is measurable through bio-impedance analysis with 50 KHZ alternating current and can be interpreted as a voltage and a wave-form current. Diabetes mellitus contains a group of metabolic disorders that are in common in terms of appearance characteristic of hyperglycemia. Micro-albuminuria of urine albumin amounts 30-300mg/24h and the higher values are for diagnostic diabetic nephropathy. The aim of this study was to determine the relationship between Phase angle and micro-albuminuria in type 2 diabetic patients with a history of more than 5 years of the disease in the city of Ilam. Materials and methods: This is an analytical-practical case-control study and statistical society includes two case and control groups with an average age 52.06 +/- 6.69 years in case group and 50.02 +/- 5.42 years in the control group. The sample size in each group has been designated equal to 50 and data analysis is performed using SPSS and parametric and Non-parametric statistical methods and the related tests. Conclusion: The average Ph. A was obtained equal to 5.87 +/- 1.42 in the case group and 7.29 +/- 0.355 in the control group which statistically significant relationship exists between the two groups in Ph. A value (P<0.000). Average micro-albuminuria was 67.32 +/- 75.92 in the case group and 0.276 +/- 0.147 in the control group which there was a significant relationship statistically between the amount of micro-albuminuria within the both groups (P< 0.000). Discussion: By investigation of the relationship between ph. Angle and micro-albuminuria in diabetic patients it can be concluded that the amount Ph. A reduces with the increase of micro-albuminuria and by increasing micro-albuminuria, the variables of overall body water volume, intracellular water and extracellular water relatively increase and fat mass relatively decreases

First isolation and identification of Vibrio vulnificus (biotype 2) from cultured beluga, huso huso in Iran

By decreasing sturgeon stocks in the Caspian Sea, rearing different sturgeon species especially Huso huso was increased in Iran. Under stress conditions the sturgeon can easily be infected by several opportunistic pathogens. In June 2011, mortality happened in 25 - 28°C water temperature, in one of the most important cultured sturgeon farms in Mazandaran province, north of Iran. The mortality rate was 15%. The first clinical signs in moribund fish were lethargy and anorexia. A total of 20 moribund fish was transferred to a central laboratory for more bacteriological examination. Clinical signs including several deep ulcers on body surface, around the head, under operculum, ulcers at the base of the pectoral fins, hemorrhage around the anus, operculum, and pale gills were observed in moribund fish. The main internal signs were hepatomegaly and splenomegaly, liquid accumulation in the intestine and diffuse visceral hemorrhage. The results of morphology and microscopic characterizations and also biochemical tests indicated that Vibrio vulnificus (biotype 2) was the etiological agent of mortality in infected fish. This study was the first report of V. vulnificus in cultured H. huso in Iran

A role for the JAK-STAT1 pathway in blocking replication of HSV-1 in dendritic cells and macrophages

<p>Abstract</p> <p>Background</p> <p>Macrophages and dendritic cells (DCs) play key roles in host defense against HSV-1 infection. Although macrophages and DCs can be infected by herpes simplex virus type 1 (HSV-1), both cell types are resistant to HSV-1 replication. The aim of our study was to determine factor (s) that are involved in the resistance of DCs and macrophages to productive HSV-1 infection.</p> <p>Results</p> <p>We report here that, in contrast to bone marrow-derived DCs and macrophages from wild type mice, DCs and macrophages isolated from signal transducers and activators of transcription-1 deficient (STAT1^-/-) mice were susceptible to HSV-1 replication and the production of viral mRNAs and DNA. There were differences in expression of immediate early, early, and late gene transcripts between STAT1^+/+and STAT1^-/-infected APCs.</p> <p>Conclusion</p> <p>These results suggest for the first time that the JAK-STAT1 pathway is involved in blocking replication of HSV-1 in DCs and macrophages.</p