88 research outputs found
A continuous model for microtubule dynamics with catastrophe, rescue and nucleation processes
Microtubules are a major component of the cytoskeleton distinguished by
highly dynamic behavior both in vitro and in vivo. We propose a general
mathematical model that accounts for the growth, catastrophe, rescue and
nucleation processes in the polymerization of microtubules from tubulin dimers.
Our model is an extension of various mathematical models developed earlier
formulated in order to capture and unify the various aspects of tubulin
polymerization including the dynamic instability, growth of microtubules to
saturation, time-localized periods of nucleation and depolymerization as well
as synchronized oscillations exhibited by microtubules under various
experimental conditions. Our model, while attempting to use a minimal number of
adjustable parameters, covers a broad range of behaviors and has predictive
features discussed in the paper. We have analyzed the resultant behaviors of
the microtubules changing each of the parameter values at a time and observing
the emergence of various dynamical regimes.Comment: 25 pages, 12 figure
Facile Preparation of Fluorescent Neoglycoproteins Using p-Nitrophenyl Anthranilate as a Heterobifunctional Linker
A facile preparation of neoglycoconjugates has been developed with a commercially available chemical, p-nitrophenyl anthranilate (PNPA), as a heterobifunctional linker. The two functional groups of PNPA, the aromatic amine and the p-nitrophenyl ester, are fully differentiated to selectively conjugate with glycans and other biomolecules containing nucleophiles. PNPA is efficiently conjugated with free reducing glycans via reductive amination. The glycan−PNPA conjugates (GPNPAs) can be easily purified and quantified by UV absorption. The active p-nitrophenyl ester in the GPNPA conjugates readily reacts with amines under mild conditions, and the resulting conjugates acquire strong fluorescence. This approach was used to prepare several fluorescent neoglycoproteins. The neoglycoproteins were covalently printed on activated glass slides and were bound by appropriate lectins recognizing the glycans
An analysis of reported motivational orientation in students undertaking doctoral studies in the biomedical sciences
Breeding for resistance to iron-deficiency chlorosis in arrowleaf clover
Last updated: 10/22/201
Breeding for resistance to iron-deficiency chlorosis in arrowleaf clover
Last updated: 10/22/201
Cooperative dynamics of microtubule ensembles: Polymerization forces and rescue-induced oscillations
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