Genetic evidence of two sibling species within the Contracoecum ogmorhini Johnson & Mawson 1941 complex (Nematoda; Anisakidae) from otariid seals in boreal and austral regions

Genetic variation of Contracaecum ogmorhini (sensu lato) populations from different otariid seals of the northern and southern hemisphere was studied on the basis of 18 enzyme loci as well as preliminary sequence analysis of the mitochondrial cyt b gene (260 bp). Samples were collected from Zalophus californianus in the boreal region and from Arctocephalus pusillus pusillus, A. pusillus doriferus and A. australis from the austral region. Marked genetic heterogeneity was found between C. ogmorhini (sensu lato) samples from the boreal and austral region, respectively. Two loci (Mdh-2 and NADHdh) showed fixed differences and a further three loci (Iddh, Mdh-1 and 6Pgdh) were highly differentiated between boreal and austral samples. Their average genetic distance was DNei = 0.36 at isozyme level. At mitochondrial DNA level, an average proportion of nucleotide substitution of 3.7% was observed. These findings support the existence of two distinct sibling species, for which the names C. ogmorhini (sensu stricto) and C. margolisi n. sp., respectively, for the austral and boreal taxon, are proposed. A description for C. margolisi n. sp. is provided. No diagnostic morphological characters have so far been detected; on the other hand, two enzyme loci, Mdh-2 and NADHdh, fully diagnostic between the two species, can be used for the routine identification of males, females and larval stages. Mirounga leonina was found to host C. ogmorhini (s.s.) inmixed infections with C. osculatum (s.l.) (of which C. ogmorhini (s.l.) was in the past considered to be a synonym) and C. miroungae; no hybrid genotypes were found,confirming the reproductive isolation of these three anisakid species. The hosts and geographical range so far recorded for C. margolisi n. sp. and C. ogmorhini (s.s.) are given

EuPRAXIA@SPARC_LAB: the high-brightness RF photo-injector layout proposal

At EuPRAXIA@SPARC_LAB, the unique combination of an advanced high-brightness RF injector and a plasma-based accelerator will drive a new multi-disciplinary user-facility. The facility, that is currently under study at INFN-LNF Laboratories (Frascati, Italy) in synergy with the EuPRAXIA collaboration, will operate the plasma-based accelerator in the external injection configuration. Since in this configuration the stability and reproducibility of the acceleration process in the plasma stage is strongly influenced by the RF-generated electron beam, the main challenge for the RF injector design is related to generating and handling high quality electron beams. In the last decades of R&D activity, the crucial role of high-brightness RF photo-injectors in the fields of radiation generation and advanced acceleration schemes has been largely established, making them effective candidates to drive plasma-based accelerators as pilots for user facilities. An RF injector consisting in a high-brightness S-band photo-injector followed by an advanced X-band linac has been proposed for the EuPRAXIA@SPARC_LAB project. The electron beam dynamics in the photo-injector has been explored by means of simulations, resulting in high-brightness, ultra-short bunches with up to 3 kA peak current at the entrance of the advanced X-band linac booster. The EuPRAXIA@SPARC_LAB high-brightness photo-injector is described here together with performance optimisation and sensitivity studies aiming to actual check the robustness and reliability of the desired working point.Comment: 5 pages,5 figures, EAAC201

Plasma boosted electron beams for driving Free Electron Lasers

In this paper, we report results of simulations, in the framework of both EuPRAXIA \cite{Walk2017} and EuPRAXIA@SPARC\_LAB \cite{Ferr2017} projects, aimed at delivering a high brightness electron bunch for driving a Free Electron Laser (FEL) by employing a plasma post acceleration scheme. The boosting plasma wave is driven by a tens of \SI{}{\tera\watt} class laser and doubles the energy of an externally injected beam up to \GeV{1}. The injected bunch is simulated starting from a photoinjector, matched to plasma, boosted and finally matched to an undulator, where its ability to produce FEL radiation is verified to yield O(\num{e11}) photons per shot at \nm{2.7}.Comment: 5 pages, 2 figure

A versatile THz source from high-brightness electron beams: Generation and characterization

Ultra-short electron bunches, such as those delivered by a high-brightness photo-injector, are suitable to produce high peak power THz radiation, both broad and narrow band, with sub-picosecond down to femtosecond pulse shaping. The features of this kind of source in the THz range of the electromagnetic spectrum are extremely appealing for frequency-and time-domain experiments in a wide variety of fields. The present manuscript will overview the method of generation and characterization of THz radiation produced by high-brightness electron beams, as those available at the SPARC_LAB test facility

Characterization of self-injected electron beams from LWFA experiments at SPARC_LAB

The plasma-based acceleration is an encouraging technique to overcome the limits of the accelerating gradient in the conventional RF acceleration. A plasma accelerator is able to provide accelerating fields up to hundreds of $GeV/m$, paving the way to accelerate particles to several MeV over a short distance (below the millimetre range). Here the characteristics of preliminary electron beams obtained with the self-injection mechanism produced with the FLAME high-power laser at the SPARC_LAB test facility are shown. In detail, with an energy laser on focus of $1.5\ J$ and a pulse temporal length (FWHM) of $40\ fs$, we obtained an electron plasma density due to laser ionization of about $6 \times 10^{18}\ cm^{-3}$, electron energy up to $350\ MeV$ and beam charge in the range $(50 - 100)\ pC$.Comment: 6 pages, 11 figures, conference EAAC201

Quadrupole scan emittance measurements for the ELI-NP compton gamma source

The high brightness electron LINAC of the Compton Gamma Source at the ELI Nuclear Physics facility in Roma- nia is accelerating a train of 32 bunches with a nominal total charge of 250 pC and nominal spacing of 16 ns . To achieve the design gamma flux, all the bunches along the train must have the designed Twiss parameters. Beam sizes are mea- sured with optical transition radiation monitors, allowing a quadrupole scan for Twiss parameters measurements. Since focusing the whole bunch train on the screen may lead to permanent screen damage, we investigate non-conventional scans such as scans around a maximum of the beam size or scans with a controlled minimum spot size. This paper discusses the implementation issues of such a technique in the actual machine layou