Brassica genomics: a complement to, and early beneficiary of, the Arabidopsis sequence.

Those studying the genus Brassica will be among the early beneficiaries of the now-completed Arabidopsis sequence. The remarkable morphological diversity of Brassica species and their relatives offers valuable opportunities to advance our knowledge of plant growth and development, and our understanding of rapid phenotypic evolution

Eyes on the account size:Interactions between attention and budget in consumer choice*

The context surrounding a consumer decision, such as one's overall budget available for pur-chases, can exert a strong effect on the subjective value of a product. Across three eye-tracking studies, we explore the attentional processes through which budget size influences consumers' purchasing behavior. Higher budgets increased and sped up purchasing even when items were affordable at all budget sizes. Moreover, attention interacted with budget size to promote pur-chasing at higher budgets. Finally, individual differences in the magnitude of the budget effect related to attentional patterns: those whose decisions depended more on budget exhibited more budget-price transitions and less variability in search patterns compared to those whose decisions were less dependent on budget. These findings indicate that attention moderates the effect of budgets on purchasing decisions, allowing low budgets to serve as self-control devices and large budgets to generate impulse purchases

FLOWERING LOCUS C -dependent and -independent regulation of the circadian clock by the autonomous and vernalization pathways

Background The circadian system drives pervasive biological rhythms in plants. Circadian clocks integrate endogenous timing information with environmental signals, in order to match rhythmic outputs to the local day/night cycle. Multiple signaling pathways affect the circadian system, in ways that are likely to be adaptively significant. Our previous studies of natural genetic variation in Arabidopsis thaliana accessions implicated FLOWERING LOCUS C (FLC) as a circadian-clock regulator. The MADS-box transcription factor FLC is best known as a regulator of flowering time. Its activity is regulated by many regulatory genes in the "autonomous" and vernalization-dependent flowering pathways. We tested whether these same pathways affect the circadian system. Results Genes in the autonomous flowering pathway, including FLC, were found to regulate circadian period in Arabidopsis. The mechanisms involved are similar, but not identical, to the control of flowering time. By mutant analyses, we demonstrate a graded effect of FLC expression upon circadian period. Related MADS-box genes had less effect on clock function. We also reveal an unexpected vernalization-dependent alteration of periodicity. Conclusion This study has aided in the understanding of FLC's role in the clock, as it reveals that the network affecting circadian timing is partially overlapping with the floral-regulatory network. We also show a link between vernalization and circadian period. This finding may be of ecological relevance for developmental programing in other plant species

Expressional regulation of PpDAM5 and PpDAM6, peach (Prunus persica) dormancy-associated MADS-box genes, by low temperature and dormancy-breaking reagent treatment

The present study investigated the expressional regulation of PpDAM5 and PpDAM6, two of the six peach (Prunus persica) dormancy-associated MADS-box genes, in relation to lateral bud endodormancy. PpDAM5 and PpDAM6 were originally identified as homologues of Arabidopsis SHORT VEGETATIVE PHASE/AGAMOUS-LIKE 24 identified in the EVERGROWING locus of peach. Furthermore, PpDAM5 and PpDAM6 have recently been suggested to be involved in terminal bud dormancy. In this study, seasonal expression analyses using leaves, stems, and lateral buds of high-chill and low-chill peaches in field conditions indicated that both genes were up-regulated during the endodormancy period and down-regulated with endodormancy release. Controlled environment experiments showed that the expression of both PpDAM5 and PpDAM6 were up-regulated by ambient cool temperatures in autumn, while they were down-regulated by the prolonged period of cold temperatures in winter. A negative correlation between expression levels of PpDAM5 and PpDAM6 and bud burst percentage was found in the prolonged cold temperature treatment. Application of the dormancy-breaking reagent cyanamide to endo/ecodormant lateral buds induced early bud break and down-regulation of PpDAM5 and PpDAM6 expression at the same time. These results collectively suggest that PpDAM5 and PpDAM6 may function in the chilling requirement of peach lateral buds through growth-inhibiting functions for bud break

Amount and time exert independent influences on intertemporal choice

Intertemporal choices involve trade-offs between the value of rewards and the delay before those rewards are experienced. Canonical intertemporal choice models such as hyperbolic discounting assume that reward amount and time until delivery are integrated within each option prior to comparison1,2. An alternative view posits that intertemporal choice reflects attribute-wise processes in which amount and time attributes are compared separately3–6. Here, we use multi-attribute drift diffusion modelling (DDM) to show that attribute-wise comparison represents the choice process better than option-wise comparison for intertemporal choice in a young adult population. We find that, while accumulation rates for amount and time information are uncorrelated, the difference between those rates predicts individual differences in patience. Moreover, patient individuals incorporate amount earlier than time into the decision process. Using eye tracking, we link these modelling results to attention, showing that patience results from a rapid, attribute-wise process that prioritizes amount over time information. Thus, we find converging evidence that distinct evaluation processes for amount and time determine intertemporal financial choices. Because intertemporal decisions in the lab have been linked to failures of patience ranging from insufficient saving to addiction7–13, understanding individual differences in the choice process is important for developing more effective interventions

Aplicación de luz UVC para esterilizar soluciones parenterales

Uno de los inconvenientes que suele presentarse al trabajar en un hospital veterinario es la necesidad de contar con un stock permanente de medicamentos y otros productos sanitarios, entre ellos, grandes volúmenes de soluciones parenterales estériles, las cuales son muy utilizadas en la clínica diaria. Los líquidos de reposición más frecuentemente empleados en medicina veterinaria son las soluciones de dextrosa al 5 %, las de cloruro de sodio al 0.9 % y las de Ringer lactato, entre otras. Es fundamental que las soluciones a administrar se encuentren en condiciones de esterilidad. En ese sentido, la luz ultravioleta de onda corta (UVC), radiación no ionizante, es un poderoso agente bactericida que desinfecta sin alterar el color, sabor, olor o pH de la muestra. El objetivo de este trabajo fue estudiar la aplicación de radiación UVC para esterilizar soluciones parenterales con almacenamiento refrigerado. Se trabajó con solución de dextrosa al 5 % y solución fisiológica al 0.9 % separadas en muestras control, sin tratar, y tratadas con luz UVC. La dosis de irradiación que recibieron las muestras fue de 3.4095 J/ cm2. Posteriormente, fueron almacenadas en cámaras de refrigeración de temperatura controlada a 4°C durante 7 días. Se observó para el caso de las muestras irradiadas, que no se presentó desarrollo visible durante ese tiempo, conservando su esterilidad. Además, se determinó el factor de inactivación, F.I. % al cabo de 7 días. En ambas soluciones estudiadas se obtuvieron entre un 99 a 100 % de inactivación. Analizando los resultados obtenidos, el uso de la irradiación con luz UVC como medio de esterilización y el posterior mantenimiento de las soluciones a temperaturas de refrigeración, consideramos que constituye una interesante y nueva tecnología a implementar.Facultad de Ciencias VeterinariasConsejo Nacional de Investigaciones Científicas y Técnica