Leukotriene B4 stimulates the release of arachidonate in human neutrophils via the action of cytosolic phospholipase A2

AbstractLeukotriene B4 (LTB4) is a potent lipid mediator of inflammation and is involved in the receptor-mediated activation of a number of leukocyte responses including degranulation, superoxide formation, and chemotaxis. In the present research, stimulation of unprimed polymorphonuclear leukocytes (neutrophils) with LTB4 results in the transient release of arachidonate as measured by mass. This release of arachidonate was maximal at an LTB4 concentration of 50–75 nM and peaked at 45 s after stimulation with LTB4. The transient nature of this release can be attributed, in part, to a fast (<60 s) metabolism of the added LTB4. Moreover, the inhibition of the reacylation of the released arachidonate with thimerosal results in greater than 4-times as much arachidonate released. Thus, a rapid reacylation of the released arachidonate also contributes to the transient nature of its measured release. Multiple additions of LTB4, which would be expected to more closely resemble the situation in vivo where the cell may come into contact with an environment where LTB4 is in near constant supply, yielded a more sustained release of arachidonate. No release of [3H]arachidonate was observed when using [3H]arachidonate-labeled cells. This indicates that the release of arachidonate as measured by mass is most probably the result of hydrolysis of arachidonate-containing phosphatidylethanolamine within the cell since the radiolabeled arachidonate is almost exclusively incorporated into phosphatidylcholine and phosphatidylinositol pools under the non-equilibrium radiolabeling conditions used. Consistent with the role of cytosolic phospholipase A2 (cPLA2) in the release of arachidonate, potent inhibition of the LTB4-stimulated release was observed with methylarachidonylfluorophosphonate, an inhibitor of cPLA2 (IC50 of 1 μM). The bromoenol lactone of the calcium-independent phosphospholipase A2. failed to affect LTB4-stimulated release of arachidonate in these cells

SDSS J210014.12+004446.0: A New Dwarf Nova with Quiescent Superhumps?

We report follow-up observations of the Sloan Digital Sky Survey Cataclysmic Variable SDSS J210014.12+004446.0 (hereafter SDSS J2100). We obtained photometry and spectroscopy in both outburst and quiescent states, providing the first quiescent spectrum of this source. In both states, non-sinusoidal photometric modulations are apparent, suggestive of superhumps, placing SDSS J2100 in the SU UMa subclass of dwarf novae. However, the periods during outburst and quiescence differ significantly, being 2.099 plus or minus 0.002 hr and 1.96 plus or minus 0.02 hr respectively. Our phase-resolved spectroscopy during outburst yielded an estimate of about 2 hr for the orbital period, consistent with the photometry. The presence of the shorter period modulation at quiescence is unusual, but not unique. Another atypical feature is the relative weakness of the Balmer emission lines in quiescence. Overall, we find a close similarity between SDSS J2100 and the well-studied superhump cataclysmic Variable V503 Cygni. By analogy, we suggest that the quiescent modulation is due to a tilted accretion disk -- producing negative superhumps -- and the modulation in outburst is due to positive superhumps from the precession of an elliptical disk.Comment: 6 pages, 5 eps figures, accepted by PASP Dec. 16th, 200

Chandra Observation of V426 Oph: Weighing the Evidence for a Magnetic White Dwarf

We report the results of a 45 ks Chandra observation of the cataclysmic variable V426 Ophiuchus. The high resolution spectrum from the high-energy transmission grating spectrometer is most consistent with a cooling flow model, placing V426 Oph among the group of CVs including U Gem and EX Hya. An uninterrupted lightcurve was also constructed, in which we detect a significant 4.2 hr modulation together with its first harmonic at 2.1 hrs. Reanalysis of archival Ginga, and ROSAT X-ray lightcurves also reveals modulations at periods consistent with 4.2 and/or 2.1 hrs. Furthermore, optical photometry in V, simultaneous with the Chandra observation, indicates a modulation anti-correlated with the X-ray, and later more extensive R band photometry finds a signal at ~2.1 hrs. The earlier reported X-ray periods at ~0.5 and 1 hrs appear to be only transient and quasi-periodic in nature. In contrast, the 4.2 hr period or its harmonic are stable and persistent in X-ray/optical data from 1988 to 2003. This periodicity is clearly distinct from the 6.85 hr orbit, and could be due to the spin of the white dwarf. If this is the case, V426 Oph would be the first long period intermediate polar with a ratio P_spin/P_orb of 0.6. However, this interpretation requires unreasonable values of magnetic field strength and mass accretion rate.Comment: 11 pages, 7 figures, 5 tables. Accepted for publication in the Astrophysical Journal (to appear August 2004