Statistical sensitivity for detection of spatial and temporal patterns in rodent population densities.

A long-term monitoring program begun 1 year after the epidemic of hantavirus pulmonary syndrome in the U.S. Southwest tracked rodent density changes through time and among sites and related these changes to hantavirus infection rates in various small-mammal reservoir species and human disease outbreaks. We assessed the statistical sensitivity of the program's field design and tested for potential biases in population estimates due to unintended deaths of rodents. Analyzing data from two sites in New Mexico from 1994 to 1998, we found that for many species of Peromyscus, Reithrodontomys, Neotoma, Dipodomys, and Perognathus, the monitoring program detected species-specific spatial and temporal differences in rodent densities; trap-related deaths did not significantly affect long-term population estimates. The program also detected a short-term increase in rodent densities in the winter of 1997-98, demonstrating its usefulness in identifying conditions conducive to increased risk for human disease

Food Habits of Rodents Inhabiting Arid and Semi-arid Ecosystems of Central New Mexico

In this study, we describe seasonal dietary composition for 15 species of rodents collected in all major habitats on the Sevilleta National Wildlife Refuge (Socorro County) in central New Mexico. A comprehensive literature review of food habits for these species from throughout their distribution also is provided. We collected rodents in the field during winter, spring and late summer in 1998 from six communities: riparian cottonwood forest; piñon-juniper woodland; juniper-oak savanna; mesquite savanna; short-grass steppe; and Chihuahuan Desert scrubland. Rodents included Spermophilus spilosoma (Spotted Ground Squirrel), Perognathus flavescens (Plains Pocket Mouse), Perognathus flavus (Silky Pocket Mouse), Dipodomys merriami (Merriams Kangaroo Rat), Dipodomys ordii (Ord\u27s Kangaroo Rat), Dipodomys spectabilis (Banner-tailed Kangaroo Rat), Reithrodontomys megalotis (Western Harvest Mouse), Peromyscus boylii (Brush Mouse), Peromyscus eremicus (Cactus Mouse), Peromyscus leucopus (White-footed Mouse), Peromyscus truei (Piñon Mouse), Onychomys arenicola (Mearn\u27s Grasshopper Mouse), Onychomys leucogaster (Northern Grasshopper Mouse), Neotoma albigula/leucodon (White-throated Woodrats), and Neotoma micropus (Southern Plains Woodrat). We collected stomach contents of all species, and cheek-pouch contents of heteromyids, and quantified them in the laboratory. We determined seasonal diets in each habitat by calculating mean percentage volumes of seeds, arthropods and green vegetation (plant leaves and stems) for each species of rodent. Seeds consumed by each rodent were identified to genus, and often species, and quantified by frequency counts. Comparisons of diets between and among species of rodents, seasons, and ecosystems were also examined. We provide an appendix of all plant taxa documented.\u2

Electronic spectra of polyatomic molecules with resolved individual rotational transitions

The density of rotational transitions for a polyatomic molecule is so large that in general many such transitions are hidden under the Doppler profile, this being a fundamental limit of conventional high resolution electronic spectroscopy. We present here the first Doppler-free cw two-photon spectrum of a polyatomic molecule. In the case of benzene, 400 lines are observed of which 300 are due to single rotational transitions, their spacing being weil below the Doppler profile. The resolution so achieved is 1.5 X 10'. Benzene is a prototype planar molecule taken to have D •• symmetry in the ground as weil as in the first excited state. From our ultra-high resolution results it is found that benzene in the excited SI state i8 a symmetrical rotor to a high degree. A negative inertial defect is found for the excited state. The origin of this inertial defect is discused

Space use and social ecology of coyotes (\u3ci\u3eCanis latrans\u3c/i\u3e) in a high-elevation ecosystem: relative stability in a changing environment

Coyote (Canis latrans) spatial and social ecology are variable, but have been little studied in high-elevation environments. In these temperate ecosystems, large ungulates are prevalent and coyote pack size may be large in order for them to scavenge and defend ungulate carcasses from conspecifics in neighboring packs. We initiated a study to understand the spatial and social ecology of coyotes on the Valles Caldera National Preserve, a high-elevation (2450–3400 m) protected area in northern New Mexico. Our objectives were to (1) describe the home range size and habitat use of coyotes in the preserve, (2) describe coyote movements within and outside of packs, and (3) to evaluate the relationship between coyote social cohesion and the amount of elk (Cervus elaphus) in the coyote diet. We acquired global positioning system and telemetry locations from 33 coyotes from August 2005 to July 2009. We classified 23 coyotes (70 % of individuals) as residents (i.e., territorial) during at least part of the study and ten coyotes (30 %) as transients. Overall mean home range size of resident packs was 10.6 ± 2.2 (SD) km2. Home range size varied between packs, but did not vary by season or year. Coyotes used dry and wet meadow habitats as expected based on availability; coyotes used riparian habitat more than expected, and forests less than expected. Social cohesion did not vary among biological seasons. Alpha coyotes were more socially cohesive with each other than with other pack members, and a transient exhibited temporal–spatial avoidance of pack members while inside the pack’s territory followed by integration into the pack. Contrary to expectations, we found no relationship between coyote social cohesion and the proportion of elk in coyote diets. We concluded that coyote space use and sociality on the preserve were relatively stable year-round despite changes in biological needs, snow depth, and utilization of variously sized prey

FOOD HABITS OF COYOTES (CANIS LATRANS) IN THE VALLES CALDERA NATIONAL PRESERVE, NEW MEXICO

Wildlife managers needed to understand coyote (Canis latrans) ecology in order to develop management plans on the nascent Valles Caldera National Preserve in northern New Mexico. Managers concerned about low elk (Cervus elaphus) recruitment had observed an increase in sightings of coyotes and observations of coyote predation on elk calves. Our objective was to identify and quantify coyote diet, and assess the temporal variation in coyote diet on the Valles Caldera National Preserve, particularly as related to elk calf consumption. We examined coyote food habits using 1,385 scats analyzed monthly from May 2005 to November 2008. The most frequent taxa were rodents (predominantly voles from the genus Microtus, and northern pocket gophers, Thomomys talpoides), elk, insects from the orders Orthoptera (grasshoppers and crickets) and Coleoptera (beetles), mountain cottontail rabbit (Sylvilagus nuttallii), and plant material (mainly grass). We detected rodent and elk in scats during all months of the study and were each present in 54% of scats overall. We identified elk remains in 43% of spring scats, 72% of winter scats, and 56% of fall scats. During summers, we could distinguish calf from adult elk hair: 8% of summer the scats contained adult elk hair and 39% contained calf elk hair. The frequency of prey items varied significantly over most seasons and years, with notable exceptions being that elk did not vary among summers and winters, and rabbits were a consistent diet item through all seasons. The high frequency of elk in the coyote diet bears further study on the density of elk calves, the vulnerability of elk to predation, the nutritional impacts from the quality of forage available to elk, and the role of hunting and other mammalian predators in providing carcasses

The I-CAN : using e-Health to get people the support they need

Background: The I-CAN is a comprehensive, reliable and valid system of identifying and classifying support needs of people with disabilities based on the conceptual framework of the International Classification of Functioning, Disability and Health (ICF) [1] and the American Association on Intellectual and Developmental Disability (AAIDD) supports concept [2,3]. Originally developed for people with Developmental Disability, the I-CAN's application of internet technologies is currently being trialled in Mental Health, Rehabilitation Medicine and other disciplines. Conceptual underpinnings, research and implementation to date are summarized. Method: ICF based domains covering Health & Well Being and Activities & Participation have been refined over several versions. A total of 1012 individuals with disabilities across the eastern states of Australia were assessed using the first three versions, and 193 with the fourth versions. Studies investigated reliability, concurrent and predictive validity and user satisfaction. A fourth internet-based version has been implemented and is under continuing investigation and refinement. Results: The I-CAN instrument demonstrated good reliability and validity in studies to date. Domain scales effectively discriminated a range of intensities of support for people with various disabilities, with highest support needs generally recorded by individuals with multiple disabilities and ageing issues. Correlations between I-CAN and adaptive behaviour scales were mixed. The ICAN scales measure individual support needs. However, regression analysis suggests that staffing and other organizational factors play a significant role in resource allocation apart from individual support need. There was general satisfaction with the assessment process from stakeholders and participants groups. Several brief case examples shall be presented. Conclusions: The I-CAN provides a reliable and valid tool for assessing and reporting on the support needs of people with disabilities using a process that involves the person, their family, friends and staff as appropriate. Several possibilities exist for this unique tool and the application of the ICF framework to e-health

Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging

Extracellular vesicles (EVs) have prevalent roles in cancer biology and regenerative medicine. Conventional techniques for characterising EVs including electron microscopy (EM), nanoparticle tracking analysis (NTA) and tuneable resistive pulse sensing (TRPS), have been reported to produce high variability in particle count (EM) and poor sensitivity in detecting EVs below 50 nm in size (NTA and TRPS), making accurate and unbiased EV analysis technically challenging. This study introduces direct stochastic optical reconstruction microscopy (d-STORM) as an efficient and reliable characterisation approach for stem cell-derived EVs. Using a photo-switchable lipid dye, d-STORM imaging enabled rapid detection of EVs down to 20–30 nm in size with higher sensitivity and lower variability compared to EM, NTA and TRPS techniques. Imaging of EV uptake by live stem cells in culture further confirmed the potential of this approach for downstream cell biology applications and for the analysis of vesicle-based cell-cell communication