Effect of Impurities on Thermal Decomposition of Calcium Carbonate

1016-101

Kinetics of Ce(IV) Oxidation of Monothioglycerol in Carbonate Medium

656-65

Reactions of Pyruvonitrile with Nb(V) & Ta(V) Alkoxides

406-40

Kinetics of Hexacyanoferrate(III) Oxidation of 4-tert-Butylthiophenol & 2-Merhyl-a-tert-butylrhiophenol

252-25

Alkoxide Complexes of Some Tri- & Tetra-valent Metals: Part I- Conductometric & Potentiometric Studies on Some Aluminium Alkoxide Complexes in Non-aqueous Solvents

256-25

Correlation of mRNA delivery timing and protein expression in lipid-based transfection

Non-viral gene delivery is constrained by the dwell time that most synthetic nucleic acid nanocarriers spend inside endosomal compartments. In order to overcome this endosomal-release bottleneck, methods are required that measure nanocarrier uptake kinetics and transfection efficiency simultaneously. Here, we employ live-cell imaging on single-cell arrays (LISCA) to study the delivery-time distribution of lipid-based mRNA complexes under varied serum conditions. By fitting a translation-maturation model to hundreds of individual eGFP reporter fluorescence time courses, the protein expression onset times and the expression rates after transfection are determined. Using this approach, we find that delivery timing and protein expression rates are not intrinsically correlated at the single-cell level, even though population-averaged values of both parameters conjointly change as a function of increasing external serum protein fraction. Lipofectamine-mediated delivery showed decreased transfection efficiency and longer delivery times with increasing serum protein concentration. This is in contrast to ionizable lipid nanoparticle (i-LNP)-mediated transfer, which showed increased efficiency and faster uptake in the presence of serum. In conclusion, the interdependences of single-cell expression rates and onset timing provide additional clues on uptake and release mechanisms, which are useful for improving nucleic acid delivery

Parametric Generation of Second Sound by First Sound in Superfluid Helium

We report the first experimental observation of parametric generation of second sound (SS) by first sound (FS) in superfluid helium in a narrow temperature range in the vicinity of $T_\lambda$. The temperature dependence of the threshold FS amplitude is found to be in a good quantitative agreement with the theory suggested long time ago and corrected for a finite geometry. Strong amplitude fluctuations and two types of the SS spectra are observed above the bifurcation. The latter effect is quantitatively explained by the discreteness of the wave vector space and the strong temperature dependence of the SS dissipation length.Comment: 4 pages, 4 postscript figures, REVTE

Observation of Crossover from Ballistic to Diffusion Regime for Excimer Molecules in Superfluid 4^4He

We have measured the temperature dependence of the time of flight of helium excimer molecules He2* in superfluid 4He and find that the molecules behave ballistically below 100mK and exhibit Brownian motion above 200 mK. In the intermediate temperature range the transport cannot be described by either of the models.Comment: 8 pages, 6 figures, submitted to the Proceedings of the International Conference on Quantum Fluids and Solids 201