Modelling of a microreactor on heterogeneous surface and an influence of microreactor geometry

A model of an action of the amperometric biosensors based on carbon paste electrodes encrusted with single microreactor is analyzed. The model is based on non stationary diffusion equations containing non-linear term related to the enzymatic reaction. The biosensors current, which is a function of the concentration gradient of the reaction product on the electrodes, is used for analyzing of dynamics of the reaction. An influence of a size of microreactor, a geometrical form of microreactor and a position of microreactor on the biosensors action is investigated

Computer Simulation of Amperometric Biosensor Response to Mixtures of Compounds

A mathematical model of amperometric biosensors has been developed. The model bases on non-stationary diffusion equations containing a non-linear term related to Michaelis-Menten kinetic of the enzymatic reaction. The model describes the biosensor response to mixtures of multiple compounds in two regimes of analysis: batch and flow injection. Using computer simulation, large amount of biosensor response data were synthesised for calibration of a biosensor array to be used for characterization of wastewater. The computer simulation was carried out using the finite difference technique

Computational Modelling of a Sensor Based on an Array of Enzyme Microreactors

This paper presents a two-dimensional-in-space mathematical model of a sensor system based an array of enzyme microreactors immobilised on a single electrode. The system acts under amperometric conditions. The model is based on the diffusion equations containing a non-linear term related to the Michaelis-Menten kinetics of the enzymatic reaction. The model involves three regions: an array of enzyme microreactors (cells) where enzyme reaction as well as mass transport by diffusion takes place, a diffusion limiting region where only the diffusion takes place, and a convective region, where the analyte concentration is maintained constant. Using computer simulation the influence of the geometry of the enzyme cells and the diffusion region on the biosensor response was investigated. The digital simulation was carried out using the finite difference technique

Nurses Alumni Association Bulletin, Fall 1995

1995-1996 Meeting Dates Calendar 1996 Annual Luncheon-Meeting Notice Officers and Committee Chairs Bulletin Publication Committee 1995-1996 Meeting Dates Calendar The President\u27s Message Financial Report What\u27s New Fiftieth Anniversary Resume of Minutes of Alumni Association Meetings Scholarship Funds at Work CAHS Alumni Board/Diploma School Alumni Office News Jefferson Health System Oldest Veteran Dies 1OOth Anniversary Pearl Harbor Remembered Memories Janet Hindson Retires Happy Birthday Scholarship Fund donors for 1994 Committee Reports By-Laws Development Bulletin Relief Fund Satellite Social Scholarship In Memoriam, Names of Deceased Graduates Class News Luncheon Photos Jefferson Alumni Identification Card The Diploma School of Nursing Alumni Association-Mabel C. Prevost Letter of Appreciation Tribute To a Mother An End Must Come Stuff For Senior Citizens to Chuckle Over Membership Application Relief Fund Application To Order: A Chronological History and Alumni Directory From TJU Bookstore Scholarship Fund Application Pins, Transcripts, Class Address List, Change of Address Forms, Alumni Identification Card Campus Map Picture - Class of 1893-189

Covalent enzyme coupling on cellulose acetate membranes for glucose sensor development

International audienceMethods for immobilizing glucose oxidase (GOx) on cellulose acetate (CA) membranes are compared. The optimal method involves covalent coupling of bovine serum albumin (BSA) to CA membrane and a subsequent reaction of the membrane with GOx, which has previously been activated with an excess of p-benzoquinone. This coupling procedure is fairly reproducible and allows the preparation of thin membranes (5-20 µm) showing high surface activities (1-3 U/cm2) which are stable over a period of 1-3 months. Electrochemical and radiolabeling experiments show that enzyme inactivation as a result of immobilization is negligible. A good correlation between surface activity of membranes and their GOx load is observed

Computer Simulation of the Response of Amperometric Biosensors in Stirred and non Stirred Solution

A mathematical model of amperometric biosensors has been developed to simulate the biosensor response in stirred as well as non stirred solution. The model involves three regions: the enzyme layer where enzyme reaction as well as mass transport by diffusion takes place, a diffusion limiting region where only the diffusion takes place, and a convective region, where the analyte concentration is maintained constant. Using computer simulation the influence of the thickness of the enzyme layer as well the diffusion one on the biosensor response was investigated. The computer simulation was carried out using the finite difference technique

Investigation of Vanillin And Its Derivatives Oxidation, Catalyzed by Laccases Isolated from Fungus

Tirta vanilino, izovanilino, vanilino rūgšties ir vanilino alkoholio oksidacija, katalizuojama iš Polyporus pinsitus, Coriolopsis byrsina ir Trichaptum abietinum grybų išskirtomis lakazėmis. Naudojant spektrofotometrinį metodą atlikti šių junginių oksidacijos kinetiniai tyrimai. Naudojant skirtingas lakazes, gaunamos santykinių pradinių sugerties kitimo greičių priklausomybės nuo substratų koncentracijų. Rezultatai, gauti naudojant skirtingas lakazes, lyginami tarpusavyje. Didžiausias tirtų junginių santykinis sugerties kitimo greitis, kuris atitinka oksidacijos greitį, buvo stebimas su Polyporus pinsitus, o mažiausias – su Trichaptum abietinum lakaze.The oxidation of vanillin and its derivatives was investigated in presence of recombinant Polyporus pinsitus, Curiolopsis byrsina and Trichaptum abietinum laccases, extracted from Basidiomycetes family fungi. Kinetic measurements were performed by using spectrophotometric method. The dependences of relative initial rate of change of absorbance on substrates concentration were obtained for various laccases. The results obtained using different laccases, compared with one another. The highest relative rate of change of investigated compounds‘ absorption, which corresponds to oxidation rate, was observed with Polyporus pinsitus laccase, while the lowest relative rate – with Trichaptum abietinum laccase. Keywords: laccase, vanillin, isovanillin, vanillic acid, vanillyl alcohol

Computer simulation of amperometric biosensor response to mixtures of compounds

A mathematical model of amperometric biosensors has been developed. The model bases on non-stationary diffusion equations containing a non-linear term related to Michaelis-Menten kinetic of the enzymatic reaction. The model describes the biosensor response to mixtures of multiple compounds in two regimes of analysis: batch and flow injection. Using computer simulation, large amount of biosensor response data were synthesised for calibration of a biosensor array to be used for characterization of wastewater. The computer simulation was carried out using the finite difference technique