Productivity and profitability of drip fertigated wheat (Triticum aestivum) – mungbean (Vigna radiata) – maize (Zea mays) cropping system

An experiment was conducted at the research farm of ICAR-Indian Agricultural Research Institute, New Delhi during 2019–20 and 2020–21 to study the productivity and economic viability of wheat (Triticum aestivum L.)– mungbean (Vigna radiata (L.) R. Wilczek)–maize (Zea mays L.) fertigated with 0, 60, 80, 100% recommended doses of NPK and irrigated at 0.6 and 0.8 crop evapotranspiration (ETc) through subsurface (SSDI) and surface drip irrigation (SDI). The results were compared with the conventional practice of surface irrigation and soil application of 100% recommended doses of NPK. Grain yields of wheat, mungbean, maize and system wheat equivalent yield (SWEY) improved by 22.9, 7.2, 21.9 and 19.4%, respectively with increase in NPK fertigation doses from 60 to 100% and by 15.6, 9.2, 4.9 and 9.7% with the increase in irrigation frequency from 0.6 to 0.8 ETc. However, SDI and SSDI had equal system productivity (12.48 and 12.85 Mg/ha). The SWEY at 0.8ETc fertigated either with NPK80 or NPK100 was statistically at par (14.2–15.9 Mg/ha) with the conventional practice (14.3–15.2 Mg/ha). The cash inflow, net income and benefit cost ratio (BCR) of the cropping system also increased successively with increase in fertigation doses and irrigation frequency. The net income and BCR followed the order maize>wheat>mungbean. The net income under SSDI at 0.8 ETc with NPK80 or NPK100 in wheat, mungbean, maize and system was 11–13, 88–105, 1-9 and 8–14% higher than the conventional practice. At 0.8 ETc and NPK100, BCR in SSDI (1.86) was higher than in SDI (1.71) and conventional system (1.67).

Probing biomolecular interaction forces using an anharmonic acoustic technique for selective detection of bacterial spores

This is the author’s version of a work that was accepted for publication in Biosensors and Bioelectronics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biosensors and Bioelectronics, 29 (1), 2011, DOI: 10.1016/j.bios.2011.08.008Receptor-based detection of pathogens often suffers from non-specific interactions, and as most detection techniques cannot distinguish between affinities of interactions, false positive responses remain a plaguing reality. Here, we report an anharmonic acoustic based method of detection that addresses the inherent weakness of current ligand dependant assays. Spores of Bacillus subtilis (Bacillus anthracis simulant) were immobilized on a thickness-shear mode AT-cut quartz crystal functionalized with anti-spore antibody and the sensor was driven by a pure sinusoidal oscillation at increasing amplitude. Biomolecular interaction forces between the coupled spores and the accelerating surface caused a nonlinear modulation of the acoustic response of the crystal. In particular, the deviation in the third harmonic of the transduced electrical response versus oscillation amplitude of the sensor (signal) was found to be significant. Signals from the specifically-bound spores were clearly distinguishable in shape from those of the physisorbed streptavidin-coated polystyrene microbeads. The analytical model presented here enables estimation of the biomolecular interaction forces from the measured response. Thus, probing biomolecular interaction forces using the described technique can quantitatively detect pathogens and distinguish specific from non-specific interactions, with potential applicability to rapid point-of-care detection. This also serves as a potential tool for rapid force-spectroscopy, affinity-based biomolecular screening and mapping of molecular interaction networks. © 2011 Elsevier B.V