1 research outputs found
PoveÄanje letalnog uÄinka bleomicina na stanice HeLa i V79 s pomoÄu pÄelinjeg otrova
This study investigated possible growth-inhibiting effects of bee venom applied alone or in combination with a cytotoxic drug bleomycin on HeLa and V79 cells in vitro based on clone formation, cell counting, and apoptosis. Melittin, the key component of bee venom, is a potent inhibitor of calmodulin activity, and also a potent inhibitor cell growth and clonogenicity. Intracellular accumulation of melittin correlates with the cytotoxicity of antitumour agents. Previous studies indicated that some calcium antagonists and calmodulin inhibitors enhanced intracellular levels of antitumor agents by inhibiting their outward transport. In this study, treatment of exponentially growing HeLa and V79 cells with bleomycin caused a dose-dependent decrease in cell survival due to DNA damage. This lethal effect was potentiated by adding a non-lethal dose of the bee venom. By preventing repair of damaged DNA, bee venom inhibited recovery from potentially lethal damage induced by bleomycin in V79 and HeLa cells. Apoptosis, necrosis, and lysis were presumed as possible mechanisms by which bee venom inhibited growth and clonogenicity of V79 cells. HeLa cells, on the other hand, showed greater resistance to bee venom. Our findings suggest that bee venom might find a therapeutic use in enhancing cytotoxicity of antitumour agent bleomycin.U uvjetima in vitro istražen je inhibitorni uÄinak pÄelinjeg otrova, samog ili združenog s citostatikom bleomicinom, na rast stanica HeLa i V79. Rabljene su sljedeÄe metode: brojenje stanica, metoda klonskog rasta i apoptoza. Poznato je da neki antagonisti kalcija i kalmodulinski inhibitori povisuju unutarstaniÄnu razinu protutumorskih lijekova inhibirajuÄi njihov prijenos iz stanice. UnutarstaniÄna akumulacija melitina izravno poveÄava citotoksiÄni uÄinak protutumorskog lijeka. Obrada stanica HeLa i V79 u eksponencijalnoj fazi rasta bleomicinom uzrokuje oÅ”teÄenje DNA ovisno o dozi te smanjenje broja živih stanica. UoÄeno je da se letalni uÄinak bleomicina može pojaÄati dodatkom neletalne doze pÄelinjeg otrova. PÄelinji otrov pritom inhibira popravak nastalih oÅ”teÄenja u stanicama HeLa i V79 te sprjeÄava oporavak stanica tretiranih bleomicinom. Apoptoza, nekroza i liza moguÄi su mehanizmi kojima pÄelinji otrov inhibira rast i stvaranje kolonija stanica V79, dok HeLa-stanice pokazuju pojaÄanu otpornost na pÄelinji otrov. Istraživanje takoÄer potvrÄuje moguÄnost uporabe pÄelinjeg otrova u poveÄanju citotoksiÄnosti bleomicina