Dynamics of dwarf-spheroidals and the dark matter halo of the galaxy

The dynamics of the dwarf-spheroidal (dSph) galaxies in the gravitational field of the Galaxy is investigated with particular reference to their susceptibility to tidal break-up. Based on the observed paucity of the dSphs at small Galactocentric distances, we put forward the hypothesis that subsequent to the formation of the Milky Way and its satellites, those dSphs that had orbits with small perigalacticons were tidally disrupted, leaving behind a population that now has a relatively larger value of its average perigalacticon to apogalacticon ratio and consequently a larger value of its r.m.s. transverse to radial velocities ratio compared to their values at the time of formation of the dSphs. We analyze the implications of this hypothesis for the phase space distribution of the dSphs and that of the dark matter (DM) halo of the Galaxy within the context of a self-consistent model in which the functional form of the phase space distribution of DM particles follows the King model, i.e. the 'lowered isothermal' distribution and the potential of the Galaxy is determined self-consistently by including the gravitational cross-coupling between visible matter and DM particles. This analysis, coupled with virial arguments, yields an estimate of ≥270 km s-1 for the circular velocity of any test object at galactocentric distances of ~100 kpc, the typical distances of the dSphs. The corresponding self-consistent values of the relevant DM halo model parameters, namely, the local (i.e., the solar neighbourhood) values of the DM density and velocity dispersion in the King model and its truncation radius, are estimated to be ~0.3 GeV cm-3, >350 km s-1 and ≥150 kpc, respectively. Similar self-consistent studies with other possible forms of the DM distribution function will be useful in assessing the robustness of our estimates of the Galaxy's DM halo parameters

Oligomerization of the human immunodeficiency virus type 1 (HIV-1) Vpu protein – a genetic, biochemical and biophysical analysis

<p>Abstract</p> <p>Background</p> <p>The human immunodeficiency virus type 1(HIV-1) is a complex retrovirus and the causative agent of acquired immunodeficiency syndrome (AIDS). The HIV-1 Vpu protein is an oligomeric integral membrane protein essential for particle release, viral load and CD4 degradation. <it>In silico </it>models show Vpu to form pentamers with an ion channel activity.</p> <p>Results</p> <p>Using Vpu proteins from a primary subtype C and the pNL4-3 subtype B isolates of HIV-1, we show oligomerization of the full-length protein as well as its transmembrane (TM) domain by genetic, biochemical and biophysical methods. We also provide direct evidence of the presence of Vpu pentamers in a stable equilibrium with its monomers <it>in vitro</it>. This was also true for the TM domain of Vpu. Confocal microscopy localized Vpu to the endoplasmic reticulum and Golgi regions of the cell, as well as to post-Golgi vesicles. In fluorescence resonance energy transfer (FRET) experiments in live cells we show that Vpu oligomerizes in what appears to be either the Golgi region or intracellular vesicles, but not in the ER.</p> <p>Conclusion</p> <p>We provide here direct evidence that the TM domain, is critical for Vpu oligomerization and the most favourable channel assembly is a pentamer. The Vpu oligomerization appears to be either the Golgi region or intracellular vesicles, but not in the ER.</p

Discovery and characterization of 91 novel transcripts expressed in cattle placenta

<p>Abstract</p> <p>Background</p> <p>Among the eutherian mammals, placental architecture varies to a greater extent than any other tissue. The diversity of placental types, even within a single mammalian order suggests that genes expressed in placenta are under strong Darwinian selection. Thus, the ruminant placenta may be a rich source of genes to explore adaptive evolutionary responses in mammals. The aim of our study was to identify novel transcripts expressed in ruminant placenta, and to characterize them with respect to their expression patterns, organization of coding sequences in the genome, and potential functions.</p> <p>Results</p> <p>A combination of bioinformatics, comparative genomics and transcript profiling was used to identify and characterize 91 novel transcripts (NTs) represented in a cattle placenta cDNA library. These NTs have no significant similarity to any non-ferungulate DNA or RNA sequence. Proteins longer than 100 aa were predicted for 29 NTs, and 21 are candidate non-coding RNAs. Eighty-six NTs were found to be expressed in one or more of 18 different tissues, with 39 (42%) showing tissue-preference, including six that were expressed exclusively in placentome. The authenticity of the NTs was confirmed by their alignment to cattle genome sequence, 42 of which showed evidence of mRNA splicing. Analysis of the genomic context where NT genes reside revealed 61 to be in intergenic regions, whereas 30 are within introns of known genes. The genes encoding the NTs were found to be significantly associated with subtelomeric regions.</p> <p>Conclusion</p> <p>The 91 lineage-specific transcripts are a useful resource for studying adaptive evolutionary responses of the ruminant placenta. The presence of so many genes encoding NTs in cattle but not primates or rodents suggests that gene loss and gain are important mechanisms of genome evolution in mammals. Furthermore, the clustering of NT genes within subtelomeric regions suggests that such regions are highly dynamic and may foster the birth of novel genes. The sequencing of additional vertebrate genomes with defined phylogenetic relationships will permit the search for lineage-specific genes to take on a more evolutionary context that is required to understand their origins and functions.</p

Expression of TNF-α and Related Signaling Molecules in the Peripheral Blood Mononuclear Cells of Rheumatoid Arthritis Patients

We examined the role of tumor necrosis factor (TNF-α) and its related signaling intermediates leading to apoptosis/proliferation in the peripheral blood mononuclear cells (PBMCs) of RA patients. The constitutive expression of mRNA for TNF-α receptors (TNFR-I and TNFR-II) and the adapter molecules, such as the TNF receptor-associated death domain protein (TRADD), Fas-associated death domain protein (FADD), receptor interacting protein (RIP), and TNF receptor-associated factor 2 (TRAF-2) were analyzed by reverse transcriptase-PCR (RT-PCR) in PBMCs from control and RA cases. PBMCs of RA patients showed a significant increase in TNF-α and TNFR-I expression as compared with that from control subjects along with significantly increased constitutive expression of TRADD, RIP, and TRAF-2 mRNA. There was a decrease in expression of FADD in RA patients, but the difference was not significant as compared to controls. These data suggested enhanced signaling by the TNFR-I-TRADD-RIP-TRAF-2 pathway and suppressed signaling by the TNFR-I-TRADD-FADD pathway in PBMCs of RA patients. However, the regulatory mechanisms for TNF-α induced signaling may not be explained only by these pathways

Jamming Attack Detection and Evaluating Using Wireless Application

When data is transferred from one host to another host,attacker may try to attack the packet or data which is in transit.Inorder to avoid such kind of attack in time critical wireless application and delivery message securely in wireless application. In this paper, we aim at modeling and detecting jamming attacks against time-critical wireless networks.To measure network performance ,packet loss and throughput metrics are used . To quantify the performance of time-critical applications,message invalidation ratio metric are used. This approach is inspired by the similarity between the behavior of a jammer who attempts to disrupt the delivery of a message and the behavior of a gambler who intends to win a gambling game. By gambling-based modeling and real-time modules, we can successful delivery time-critical message under a variety of jamming attacks. DOI: 10.17762/ijritcc2321-8169.15038