Antibiofilm activity of LAE (ethyl lauroyl arginate) against food-borne fungi and its application in polystyrene surface coating

Several filamentous fungi species as Fusarium oxysporum or Cladosporium sp. can form biofilms by themselves or by participating in polymicrobial biofilms with bacteria. However, despite the high impact of biofilm on the food industry and the high efforts done to control biofilm produced by bacteria in the food area, there has been little study of strategies to control fungal biofilm in this area. In this study, the antibiofilm activity of the safe antimicrobial compound ethyl lauroyl arginate (LAE) was investigated against food spoilage fungi (Cladosporium cladosporioides, Aspergillus ochraceus, Penicillium italicum, Botrytis cynerea and Fusarium oxyspoum). Finally, the efficacy of a varnish-based coating incorporating LAE and coated onto polystyrene microtiter plates has been evaluated as a strategy to reduce fungal biofilm formation. The results of the 2,3-bis-(2-metoxi-4-nitro-5-sulfofenil)-2H-tetrazoilo-5-carboxanilida (XTT) assay, which measure the biofilm metabolic activity of moulds, demonstrated that LAE reduced significantly the formation of fungal biofilm at concentrations from 6 to 25 mg/L. This reduction was confirmed by the micrographs obtained by scanning electronic microscopy (SEM). In addition, LAE also showed antifungal activity against established biofilms. Particularly, it reduced their metabolic activity and viability at concentrations from 6 to 25 mg/L according to results obtained in the XTT assay and observations made by confocal laser scanning microscopy (CLSM). Finally, active coating incorporating from 2% of LAE proved to reduce significantly the biofilm formation in C. cladosporioides, B. cynerea and F. oxyspoum according to the results obtained in the XTT assay. However, the released studies indicated that the retention of LAE in the coating should be improved to prolong their activity

Bring some colour to your package: freshness indicators based on anthocyanin extracts

Background: Nowadays, consumers are more aware about what they eat and how it is packaged. Food quality is of paramount importance and consumers are eager to be constantly updated about the perishability and shelf-life of the food products they buy. This gave rise to intelligent packaging (IP) materials and devices that can communicate with all people throughout the entire food route. One of the main colour-based IP systems is based on the use of anthocyanin (ATH)-based natural dyes by exploiting their pH-dependent colour changes that can be easily readable by the naked eye. Scope and approach: This review focuses on recent studies regarding ATH-based sensor packaging materials development for IP applications, offering valuable insights regarding the main factors that affect the efficacy of the developed IP, such as the material where the ATH is anchored, ATH type and concentration, the ingredients used in the formulation of the sensor and the storage conditions of the food product. Additionally, the last ATH-based IP developed are also summed and discussed in detail to identify the major bottlenecks that need to be overcome for accelerating the commercial application of ATH-based sensors in IP. Key findings and conclusions: Although ATH-based indicators are able to correctly indicate food shelf-life in several products at a laboratory scale, their industrial production is still impaired by the production methods used for this type of sensors. Furthermore, ATHs are labile compounds that require stabilization in the packaging material to yield stable, standardized and durable IP materials

The impact of improved maize germplasm on poverty alleviation

"This study documents how poor small-scale farmers in lowland tropical Mexico use improved maize germplasm and how this contributes to their well-being. It does this by assessing both the direct adoption of improved varieties and examining the process of their "creolization." By exposing improved varieties to their conditions and management, continually selecting seed of these varieties for replanting, and in some cases promoting their hybridization with landraces, either by design or by accident, farmers produce what they recognize as "creolized" varieties. Our key hypothesis is that poor farmers benefit from improved germplasm through creolization. Creolization provides farmers with new options, as they deliberately modify an improved technology generated by the formal research system to suit their own circumstances and needs. Different methodologies such as participatory methods, ethnography, household case studies, a household sample survey, and a collection and agronomic evaluation of maize samples were used. This study was carried out in two regions: the coast of Oaxaca and the Frailesca in the states of Oaxaca and Chiapas, two of the poorest in Mexico. While one study area is subsistence-oriented and the other commercial, in both, extreme poverty is pervasive. Maize continues to play a key role in the livelihoods of the poor in both study areas. Authors' AbstractSustainable livelihoods ,plant breeding ,Hybrid maize Mexico ,Agricultural innovations ,

The impact of improved maize germplasm on poverty alleviation

"This study documents how poor small-scale farmers in lowland tropical Mexico use improved maize germplasm and how this contributes to their well-being. It does this by assessing both the direct adoption of improved varieties and examining the process of their "creolization." By exposing improved varieties to their conditions and management, continually selecting seed of these varieties for replanting, and in some cases promoting their hybridization with landraces, either by design or by accident, farmers produce what they recognize as "creolized" varieties. Our key hypothesis is that poor farmers benefit from improved germplasm through creolization. Creolization provides farmers with new options, as they deliberately modify an improved technology generated by the formal research system to suit their own circumstances and needs. Different methodologies such as participatory methods, ethnography, household case studies, a household sample survey, and a collection and agronomic evaluation of maize samples were used. This study was carried out in two regions: the coast of Oaxaca and the Frailesca in the states of Oaxaca and Chiapas, two of the poorest in Mexico. While one study area is subsistence-oriented and the other commercial, in both, extreme poverty is pervasive. Maize continues to play a key role in the livelihoods of the poor in both study areas. Authors' AbstractSustainable livelihoods ,plant breeding ,Hybrid maize Mexico ,Agricultural innovations ,

Comprehensive transient-state study for CARMENES-NIR high thermal stability

CARMENES has been proposed as a next-generation instrument for the 3.5m Calar Alto Telescope. Its objective is finding habitable exoplanets around M dwarfs through radial velocity measurements (m/s level) in the near-infrared. Consequently, the NIR spectrograph is highly constraint regarding thermal/mechanical requirements. As a first approach, the thermal stability has been limited to \pm 0.01K (within year period) over a working temperature of 243K. This can be achieved by means of several temperature-controlled rooms. The options considered to minimise the complexity of the thermal design are here presented, as well as the transient-state thermal analyses realised to make the best choice